The invention relates to a plant source preparation for antiviral infection for livestock and poultry, in particular to the application of HHT as a broad-spectrum antiviral preparation. The present invention finds for the first time that homoharringtonine (HHT) has broad-spectrum and high-efficiency antiviral activity, and can efficiently inhibit 9 kinds of viruses (VSV, NDV, PEDV, TGEV, AIV, HSV‑1, PRV, PRRSV, FMDV) ) to infect host cells and animals (chicken, chicken embryos, pigs, etc.), significantly reduce the amount of virus in the animal body, and alleviate the symptoms of infection, providing safe and reliable new biological agents for the breeding industry. The invention provides the basis and possibility for developing broad-spectrum and high-efficiency antiviral new drugs, and provides a new safe and reliable technical means for ensuring the sustainable development of agriculture and animal husbandry and human health. It has far-reaching practical significance for developing traditional medical resources and promoting the cause of traditional Chinese medicine in our country.