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Application of alkaloid in cephalataxus fortunei esters for preventing and curing nematodiasis of plants

A plant nematode prevention and control technology, which is applied in the field of harringtonine alkaloids, can solve the problems such as the lack of significant advantages in the nematodecidal effect of alkaloid alkaloids and the lack of nematicidal active ingredients, and achieve the goal of easy degradation and low residual environment Effect

Inactive Publication Date: 2005-08-31
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the 1970s, due to the awareness of the harmfulness of chemical pesticides to the environment, the international community has gradually paid attention to finding natural nematode active substances from plants, such as India, Japan, Italy, the United States, the United Kingdom and other countries have research in this area However, most of them are still in the preliminary screening stage, and few effective nematode active ingredients have been found, which is still far away from practical application.
However, compared with chemical nematocides, the nematicidal effect of oxaconidinium does not have significant advantages

Method used

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  • Application of alkaloid in cephalataxus fortunei esters for preventing and curing nematodiasis of plants
  • Application of alkaloid in cephalataxus fortunei esters for preventing and curing nematodiasis of plants
  • Application of alkaloid in cephalataxus fortunei esters for preventing and curing nematodiasis of plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: Preparation of Harringtonine and Homoharringtonine

[0024] Cephalotaxus oliveri branch dry coarse powder was extracted three times with 8 times the amount of 95% ethanol, the extract was concentrated to recover the ethanol to obtain a concentrated water solution, and 3mol / L hydrochloric acid was added to adjust to pH 3, filtered, and the filtrate was sodium carbonate Alkalize to pH 9-10, extract with chloroform, and recover chloroform to obtain total alkaloids (yield 0.053%). The total alkaloids are dissolved in 4% citric acid water, and the acid water solution is then adjusted to pH 6, 7, 8, 9 sequentially with saturated sodium carbonate solution, and extracted with chloroform to obtain pH 6-7 and pH 8-9 respectively. The pH 8-9 part is passed through a silica gel column, eluted with chloroform-methanol (98:2), and then recrystallized with ether to obtain harringtonine. Pass through a silica gel column at pH 6-7, eluting with a gradient of chloroform-methanol, ...

Embodiment 2

[0025] Example 2: Identification of Harringtonine and Homoharringtonine

[0026] Harringtonine: molecular formula C 28 H 37 NO 9 ; Colorless crystals, mp74~76℃ (ether); [α] 25 D -105°(c0.5, CHCl 3 ). In HPTLC (Kieselgel 60GF 254 , Merck) is consistent with the harringtonine standard; mixed with the harringtonine standard to crystallize, the melting point does not decrease. IR, 1 H NMR and 13 The C NMR data are consistent with the reported values ​​of harringtonine.

[0027] Homoharringtonine: molecular formula C 29 H 39 NO 9 ; Colorless crystal, mp144~146℃; [α] 25 D -119°(c0.5, CHCl 3 ). In HPTLC (Kieselgel 60GF 254 , Merck) is consistent with the homoharringtonine standard; mixed with the homoharringtonine standard to crystallize, the melting point does not decrease. IR, 1 H NMR and 13 The C NMR data are consistent with the reported values ​​of homoharringtonine.

Embodiment 3

[0028] Example 3: Determination of virulence to pine wood nematode

[0029] Dissolve harringtonine, homoharringtonine, ethnoxine and Ethoprop granules with a small amount of acetone, dilute with distilled water, and prepare test solutions of different concentrations (μg / mL). In a petri dish with a diameter of 6 cm, PDA medium was used to inoculate Pestalotia sp. and cultured in a constant temperature incubator at 25° C. for 5 days until the hyphae became full. Place a small square piece of absorbent cotton with a length of about 1 cm and a thickness of about 0.5 cm in the middle of the petri dish covered with the hyphae of Polychaete spp. Use a micro syringe to draw 0.5 ml of the test solution of different concentrations and inject it into the cotton block, and then add 0.1 ml of Bursaphelenchus xylophilus solution to the center of the cotton block. Each treatment is repeated 4 times, and a 2% acetone aqueous solution is used as a control. . Culture the nematodes in a constant tem...

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Abstract

An application of the cephalotaxine ester, especially the harringtonine and homoharringtonine, in preventing and treating the roundworm diseases of plant is disclosed.

Description

Technical field [0001] The present invention relates to the use of Cephalotaxine esters (Cephalotaxine esters), especially Harringtonine (Harringtonine) and Homoharringtonine (Homoharringtonine) (Harringtonine or homoharringtonine can be used alone , A mixture of two compounds consisting of harringtonine and homoharringtonine can also be used, especially for the purpose of preventing and controlling plant nematode diseases. Background technique [0002] Plant nematodes are a kind of common plant pathogenic pests, which are widely distributed and host wide, which have caused serious damage to agriculture and forestry production. Especially in the past ten years, the invasion of Bursaphelenchus xylophilus (Bursaphelenchus xylophilus) has caused huge losses to the forestry production of East Asian countries (including my country). Chemical nematicides are effective measures to prevent and control plant nematode diseases, but chemical nematicides have the shortcomings of high toxicit...

Claims

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Application Information

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IPC IPC(8): A01N43/90C07D491/02
Inventor 张凤仙刘梅芳文艳华叶万辉魏孝义
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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