Application of alkaloid in cephalataxus fortunei esters for preventing and curing nematodiasis of plants

A plant nematode and control technology, which is applied in the field of harringtonine alkaloids, can solve the problems of few nematicidal active ingredients and the nematicidal effect of torrenine has no significant advantages, and achieves easy degradation and low residual environment. Effect

Inactive Publication Date: 2006-06-14
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the 1970s, due to the awareness of the harmfulness of chemical pesticides to the environment, the international community has gradually paid attention to finding natural nematode active substances from plants, such as India, Japan, Italy, the United States, the United Kingdom and other countries have research in this area However, most of them are still in the preliminary screening stage, and few effective nematode active ingredients have been found, which is still far away from practical application.
However, compared with chemical nematocides, the nematicidal effect of oxaconidinium does not have significant advantages

Method used

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  • Application of alkaloid in cephalataxus fortunei esters for preventing and curing nematodiasis of plants
  • Application of alkaloid in cephalataxus fortunei esters for preventing and curing nematodiasis of plants
  • Application of alkaloid in cephalataxus fortunei esters for preventing and curing nematodiasis of plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1: Preparation of harringtonine and homoharringtonine

[0024] Cephalotaxus oliveri twig coarse powder, leached three times with 8 times the amount of 95% ethanol, concentrated the leaching solution to recover the ethanol to obtain a concentrated aqueous solution, added 3mol / L hydrochloric acid to adjust to pH 3, filtered, and the filtrate was distilled with carbonic acid Alkalinize with sodium to pH 9-10, extract with chloroform, recover chloroform, and obtain total alkaloids (yield: 0.053%). The total alkaloids were dissolved in 4% citric acid water, and the acid water solution was then adjusted to pH 6, 7, 8, and 9 with saturated sodium carbonate solution, and extracted with chloroform to obtain two parts with pH 6-7 and pH 8-9 respectively. Pass through a silica gel column at pH 8-9, elute with chloroform-methanol (98:2), and then recrystallize with ether to obtain harringtonine. Pass through a silica gel column at pH 6 to 7, elute with chloroform-methan...

Embodiment 2

[0025] Example 2: Identification of harringtonine and homoharringtonine

[0026] Harringtonine: molecular formula C 28 h 37 NO 9 ;Colorless crystal, mp 74~76℃(ether);[α] 25 D -105°(c0.5, CHCl 3 ). In HPTLC (Kieselgel 60GF 254 , Merck) is consistent with the harringtonine standard; mixed with the harringtonine standard for crystallization, the melting point does not decrease. IR, 1 H NMR and 13 The C NMR data were in good agreement with the values ​​reported in the literature for harringtonine.

[0027] Homoharringtonine: molecular formula C 29 h 39 NO 9 ;Colorless crystal, mp 144~146℃;[α] 25 D -119°(c0.5, CHCl 3 ). In HPTLC (Kieselgel 60GF 254 , Merck) is consistent with the homoharringtonine standard; mixed with the homoharringtonine standard for crystallization, the melting point does not decrease. IR, 1 H NMR and 13 The C NMR data agree well with the values ​​reported in the literature for homoharringtonine.

Embodiment 3

[0028] Embodiment 3: To the mensuration of pine xylophilus toxicity

[0029] Dissolve harringtonine, homoharringtonine, corretine and Ethoprop granules in a small amount of acetone, dilute with distilled water, and prepare test solutions with different concentrations (μg / mL). In a petri dish with a diameter of 6 cm, PDA medium was used to inoculate Pestalotia sp., and cultured in a constant temperature incubator at 25° C. for 5 days until the hyphae covered the dish. Place a piece of small square absorbent cotton piece with a length of about 1 cm and a thickness of about 0.5 cm in the middle of the petri dish covered with polychaete mycelium. Use a micro-syringe to draw 0.5 ml of different concentrations of the test solutions, inject them into the cotton block, and then draw 0.1 ml of pine wood nematode (Bursaphelenchus xylophilus) solution and add it to the center of the cotton block. Set 4 repetitions for each treatment, and use 2% acetone aqueous solution as a control . T...

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Abstract

The present invention relates to the application of cephalotaxine esters (Cephalotaxine esters) of the following general formula (1) to control plant nematode diseases. In particular, Harringtonine in which x is 2 and Homoharringtonine in which x is 3 have a strong poisonous effect on plant nematodes and can be effectively used for the prevention and treatment of plant nematode diseases. ∴

Description

technical field [0001] The present invention relates to the purposes of cephalotaxine esters (Cephalotaxine esters), especially harringtonine (Harringtonine) and homoharringtonine (Homoharringtonine) (can use cephalotaxine or homoharringtonine alone , a mixture of two compounds, harringtonine and homoharringtonine, can also be used), especially for the prevention and treatment of plant nematode diseases. Background technique [0002] Plant nematodes are a common plant pathogenic pest with a wide distribution and a wide range of hosts, causing serious harm to agricultural and forestry production. Especially in the past ten years, the invasion of pine wood nematode (Bursaphelenchus xylophilus) has caused huge losses to the forestry production of East Asian countries (including my country). Chemical nematicides are effective measures to prevent and control plant nematode diseases, but chemical nematicides have the disadvantages of high toxicity and high residue. Long-term use ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D487/04A01N43/90C07D491/02
Inventor 张凤仙刘梅芳文艳华叶万辉魏孝义
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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