Application of natural compound Homoharringtonine in preparation of tumor treatment medicine

A tumor and tumor cell technology, applied in the direction of anti-tumor drugs, active ingredients of heterocyclic compounds, drug combinations, etc., can solve the problems of treatment failure and limited effect, and achieve the effect of promoting survival and good curative effect.

Inactive Publication Date: 2015-11-11
SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, after a period of use, most patients develop drug resistance due to the mutation of epithelial growth factor receptor (EGFR) T790M, which

Method used

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  • Application of natural compound Homoharringtonine in preparation of tumor treatment medicine
  • Application of natural compound Homoharringtonine in preparation of tumor treatment medicine
  • Application of natural compound Homoharringtonine in preparation of tumor treatment medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1, HHT inhibits the proliferation of non-small cell lung cancer and induces tumor cell apoptosis

[0047] 1. HHT inhibits the proliferation of non-small cell lung cancer

[0048] 1.1 HHT inhibits the proliferation of A549 cells

[0049] The experiment was repeated three times, and the specific steps of each repeated experiment were as follows:

[0050] A549 cells were divided into seven groups, each group 3 × 10 3 cells, these seven groups of A549 cells were processed according to the following method to obtain seven A549 cell culture fluids processed through different concentrations of HHT:

[0051] One group was in DMEM medium containing 10% fetal bovine serum in 5% CO 2 After culturing at 37°C for 20 hours in an incubator, add 10 μL of MTT aqueous solution with a concentration of 5 mg / mL and continue to cultivate for 4 hours, then suck out the medium containing MTT, add 150 μL DMSO, and place on a decolorization shaker at room temperature in the dark Shak...

Embodiment 2

[0098] Example 2, HHT induces apoptosis of non-small cell lung cancer cells through the mitochondrial apoptosis pathway

[0099] The experiment was repeated three times, and the specific steps of each repeated experiment were as follows:

[0100] Inoculate A549 cells into a 6-well plate containing DMEM medium, 1mL DMEM medium per well, 2×10 per well 5 A549 cells in 5% CO 2 Cultivate overnight at 37°C in an incubator to obtain the A549 cell culture solution after the cells adhere to the wall; add HHT to the A549 cells to obtain the HHT-A549 cell culture solution, and the concentration of HHT in the HHT-A549 cell culture solution is 4 μM; HHT-A549 cell culture medium in 5% CO 2 Incubate in an incubator at 37°C for 24 hours, discard the medium, wash the cells twice with 1×PBS, and then lyse the cells with RIPA lysis solution (RIPA lysis solution is a product of Beyond Biotechnology Co., Ltd.; product number P0013B) (each 200 μL RIPA lysate in the well), then centrifuged at 1,2...

Embodiment 3

[0106] Example 3, HHT inhibits STAT3 phosphorylation, thereby inhibiting STAT3 activation, from inhibiting cell survival

[0107] STAT3 expression levels and For its phosphorylation state, the primary antibody to detect the expression level of STAT3 is Anti-STAT3antibody[EPR787Y] (Abcam product, product number is ab68153), and the primary antibody to detect the phosphorylation state of STAT3 is Anti-STAT3(phosphoS727)antibody[E121-31 ] (Abcam product, product number is ab32143) and Anti-STAT3 (phosphoY705) antibody [EP2147Y] (Abcam product, product number is ab76315), the internal reference is GAPDH, and the primary antibody of GAPDH is Anti-GAPDHrabbitpolyclonalantibody (Sangon Bioengineering (Shanghai) Co., Ltd. Co., Ltd. products, the article number is D110016-0200), the results are as follows image 3 Shown in A.

[0108]The results showed that HHT inhibited the phosphorylation of STAT3Thr705, but did not affect the phosphorylation of STAT3Ser727 and the expression level...

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Abstract

The invention discloses application of a natural compound Homoharringtonine in preparation of a tumor treatment medicine. Homoharringtonine of the invention has following characteristics of: inhibiting transcriptional activity of a transcription factor STAT3, inhibiting expression of a downstream gene MCL1 and survivin of STAT3, and inhibiting an anti-apoptosis effect of the MCL1 and surviving. Homoharringtonine has an action of effectively resisting tumor proliferation and resisting STAT3 phosphorylation in non-small cell lung cancer subcutaneous tumor bearing migration model of a nude mice. As a natural compound, Homoharringtonine can overcome the promotion action of interleukin IL-6 to tumors. The application achieves a new level in tumor targeted therapy aspect of natural compounds, and has well application prospect.

Description

technical field [0001] The invention relates to the application of natural compound Homoharringtonine in the preparation of tumor treating medicine in the field of biomedicine. Background technique [0002] Lung cancer is one of the most common tumors, and about one quarter of cancer patients die from lung cancer every year. According to statistics, in 2014, there were 224,210 new lung cancer patients (including 116,000 male patients and 108,210 female patients) and 159,260 deaths (including 86,930 male patients and 72,330 female patients) in 2014. Lung cancer is generally divided into two types: small cell lung cancer (SCLC) and non-small cell lung cancer (non-small cell lung cancer, NSCLC), among which non-small cell lung cancer is the most common, accounting for about 80% of the total number of lung cancer cases. According to the development stage of the disease and the pathological conditions, the methods for the treatment of lung cancer mainly include surgical chemothe...

Claims

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Application Information

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IPC IPC(8): A61K31/55A61P35/00A61P35/02A61P11/00
Inventor 黄来强曹威刘莹
Owner SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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