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Sample pretreatment method for detecting sodium thiocyanate in liquid food

A sample pretreatment and liquid food technology, applied in the preparation of test samples, etc., can solve the problems of large sample pretreatment error, poisonous and harmful to human body, and influence on test results, and achieve less impurities, high accuracy and precision, good reproducibility

Inactive Publication Date: 2018-06-08
安徽宏远职业卫生技术服务有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Sodium thiocyanate has a certain antibacterial effect, but it is a non-edible additive substance that is toxic and harmful to the human body. Since the content of additives in liquid food is small and the composition is complex, it will affect the test results, so the sample pretreatment is generally It takes a long time and many steps, resulting in large sample pretreatment errors, so it is necessary to provide a new sample pretreatment method for the detection of sodium thiocyanate in liquid food

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A kind of sample pretreatment method that the present invention proposes detects sodium thiocyanate in liquid food, comprises the following steps:

[0019] S1. Take 10mL liquid food sample and place it in a conical flask, add 2mL trichloroacetic acid solution, stir while adding, mix well and let it stand for 15min, filter it with quantitative filter paper in a clean and dry conical flask to obtain supernatant A. use;

[0020] S2. Add 20ml ultrapure water to the supernatant A to dissolve, transfer the supernatant A dilution to a 50mL volumetric flask in 3 times, extract by ultrasonic vibration for 8min, take it out, and the ultrasonic power is 40W, add the precipitate to the volumetric flask respectively The precipitating agent is mixed with 0.5mL potassium ferrocyanide, 0.5mL zinc acetate, and 0.5mL ammonium sulfate, vortexed for 1min, fixed to volume with ultrapure water and mixed evenly, placed in a centrifuge tube for centrifugation, centrifuged The time is 10 minut...

Embodiment 2

[0022] A kind of sample pretreatment method that the present invention proposes detects sodium thiocyanate in liquid food, comprises the following steps:

[0023] S1. Take 15mL liquid food sample and place it in a conical flask, add 5mL trichloroacetic acid solution, stir while adding, mix well and let it stand for 25min, filter it with quantitative filter paper in a clean and dry conical flask to obtain supernatant A. use;

[0024] S2. Add 30ml ultrapure water to the supernatant A to dissolve, transfer the supernatant A dilution to a 50mL volumetric flask in 5 times, extract by ultrasonic vibration for 15min, and take it out, the ultrasonic power is 50W, and add the precipitate to the volumetric flask respectively Precipitating agent, the precipitating agent is made by mixing 1.5mL potassium ferrocyanide, 1.5mL zinc acetate, and 1.5mL ammonium sulfate, vortex precipitation for 2min, use ultrapure water to make up volume and mix evenly, place in a centrifuge tube for centrifug...

Embodiment 3

[0026] A kind of sample pretreatment method that the present invention proposes detects sodium thiocyanate in liquid food, comprises the following steps:

[0027] S1. Take 12mL liquid food sample and place it in a conical flask, add 3mL trichloroacetic acid solution, stir while adding, mix well and let it stand for 20min, filter it with quantitative filter paper in a clean and dry conical flask to obtain supernatant A. use;

[0028] S2. Add 25ml ultrapure water to the supernatant A to dissolve, transfer the supernatant A dilution to a 50mL volumetric flask in 4 times, extract by ultrasonic vibration for 11min, and take it out after ultrasonic power is 45W, add the precipitate to the volumetric flask respectively The precipitating agent is composed of 1mL potassium ferrocyanide, 1mL zinc acetate, and 1mL ammonium sulfate, vortex for 2min, use ultrapure water to make up the volume and mix evenly, put it in a centrifuge tube for centrifugation, and the centrifugation time is 13mi...

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Abstract

The invention discloses a sample pretreatment method for detecting sodium thiocyanate in liquid food, and relates to the technical field of food detection. The method comprises the following steps oftaking liquid food samples; putting the liquid food samples into a triangular flask; adding a trichloroacetic acid solution; standing after the uniform mixing; filtering to obtain supernate A for later use; adding super-pure water into the supernate A for dissolution and dilution; transferring the materials into a volume bottle; performing ultrasonic oscillation; respectively adding precipitants into the volume bottle for vortex precipitation; reaching constant volume by super-pure water; performing centrifugation treatment; sucking supernate B; using a microporous film for filtering, so as toobtain a sample solution. The method can be used for effectively removing impurities such as protein, fat and grease in the liquid food; the pretreatment is simple; the reproduction quality is high;the sample pretreatment time is shortened; after the treatment, the impurities are few; the detection accuracy and precision are high; the fast detection requirements of a great number of samples canbe met.

Description

technical field [0001] The invention relates to the technical field of food detection, in particular to a sample pretreatment method for detecting sodium thiocyanate in liquid food. Background technique [0002] Food safety is a hot and sensitive issue of concern to the world, and it is related to the personal and property safety of the people. In recent years, food safety issues have occurred frequently. Dozens of food safety incidents such as sodium thiocyanate, melamine, Sudan red, carcinogenic poisonous rice, Fuyang Datouwa milk powder, Formosa Plastics, and clenbuterol were investigated and exposed. of great concern. At present, food safety issues mainly include the following aspects: chemical hazards, biological toxins, microbial hazards, food adulteration, bio-transgenic foods, and bioterrorism incidents. In response to these aspects, my country has also issued various food safety standards and a series of general analysis methods for food safety. The detection ite...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/34
CPCG01N1/34
Inventor 夏怀翔赵华芝孔玉萍严章飞方栋张强段志祥
Owner 安徽宏远职业卫生技术服务有限公司
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