Pesticide detection method based on fluorescence analysis

A detection method and fluorescence analysis technology, applied in the field of pesticide detection based on fluorescence analysis, can solve the problems of inability to optimize the detection and analysis of different types of pesticides, and fewer fluorescence analysis systems

Active Publication Date: 2021-07-30
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are few fluorescence analysis systems applied to the in situ detection of pesticide residues in animals and plants, and most of the existing methods are for specific pesticides (such as organophosphor

Method used

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  • Pesticide detection method based on fluorescence analysis
  • Pesticide detection method based on fluorescence analysis
  • Pesticide detection method based on fluorescence analysis

Examples

Experimental program
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Effect test

preparation example Construction

[0039] The preparation steps of formula I compound are as follows:

[0040] Dissolve 4-methoxy-o-hydroxyacetophenone and p-dimethylaminobenzaldehyde in a mixed solvent of ethanol and water, wherein the water content is 30-50wt%, add 10ml of NaOH aqueous solution (10-20wt%), and Stir at 80°C for 18-24 hours, pour the reaction liquid into 1000ml of water and add acid to neutralize, and obtain 4MC through recrystallization. Compound structural characterization data such as Figure 5 shown.

[0041] Chalcone CD1 (its structural formula is as shown in formula II) is purchased,

[0042]

[0043] Formula II,

[0044] Purchased from energy-chemical, product number D5312 (CAS number: 22965-98-6), purchase website link https: / / www.energy-chemical.com / front / search.htm? type=anjCas&keyword=D5312.

[0045] Chalcone DNC (its structural formula is as shown in formula III) is self-prepared,

[0046]

[0047] Formula III,

[0048] The preparation steps of formula III compound are ...

Embodiment 1

[0071] Preparation of protein-fluorescent probe system.

[0072] Taking the combination of human serum albumin (HSA) and chalcone 4MC fluorescent probe as an example, the specific preparation method of the protein-fluorescent probe system is as follows:

[0073] Dissolve 66.5 mg of human serum albumin (HSA) in 1 ml of PBS buffer solution (pH=7.4, 0.1X), shake at room temperature for 1 min to obtain a uniform concentration of 1×10 -3 mol / L protein stock solution. Dissolve 3.0mg of chalcone 4MC in 5ml of DMSO, sonicate for 1min to obtain a concentration of 2×10 -3 mol / L chalcone 4MC fluorescent probe mother solution. Pipette 20 μL of the protein mother solution into 2 ml of PBS buffer solution, add 10 μL of the chalcone 4MC fluorescent probe mother solution under shaking conditions, continue to shake for 1 minute and then let it stand for 2 minutes to obtain a protein-fluorescent probe system, human serum albumin (HSA) and chalcone The concentration of the ketone 4MC fluoresc...

Embodiment 2

[0081] Preparation of protein-fluorescent probe-pesticide system.

[0082] The pesticide was dissolved in 2ml DMSO to obtain a pesticide mother solution with a concentration of 50mM. Add different volumes of pesticide mother liquors to the protein-fluorescent probe system obtained in Example 1 (both protein and probe concentrations are 10 μM), and a series of proteins with pesticide addition concentrations of 0-1 mM (0-100 e.q.) are obtained. - Fluorescent probe-pesticide system.

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Abstract

The invention discloses a pesticide detection method based on fluorescence analysis. According to the pesticide detection method, an organic fluorescent probe is combined with the protein to form a fluorescence detection system with a host-guest structure, and detection is realized according to the quantitative relationship between the generated fluorescence signal and the pesticide concentration by utilizing a competitive ligand replacement reaction between the pesticide with stronger binding force and the organic fluorescent probe. The pesticide detection method disclosed by the invention is low in cost, high in sensitivity and short in response time, and meets the requirement of in-vitro rapid detection; meanwhile, the fluorescence analysis method has the advantages of non-invasive detection and visualization, and is particularly suitable for carrying out in-situ detection on pesticide residues in animal and plant bodies.

Description

technical field [0001] The invention belongs to the technical field of pesticide residue detection, and in particular relates to a pesticide detection method based on fluorescence analysis. Background technique [0002] Pesticides are chemical agents used in modern agriculture to prevent and control plant diseases and insect pests and regulate plant growth. They are classified according to chemical structure, including organophosphorus, organochlorine, carbamate, benzopyrazole, pyrethroid, etc., and are classified according to function. Insecticides, herbicides, fungicides, acaricides, plant growth regulators, etc. The wide use of pesticides avoids the attack of pests, weeds, and bacteria for plant growth, but while improving the efficacy of the pesticides, its high biological toxicity can cause poisoning of bees, silkworms, fish and other animals, and the residual pesticides in animals and plants If it is too high, it will cause food safety problems. Therefore, it is of g...

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N1/38
CPCG01N21/643G01N1/38G01N2021/6417
Inventor 徐汉虹秦天依赵晨
Owner SOUTH CHINA AGRI UNIV
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