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Clenbuterol hydrochloride assay kit and its preparation method and use method

A technology of clenbuterol hydrochloride and detection kit, which is applied in the field of clenbuterol detection, can solve problems such as time-consuming and complicated operation, and achieve the effects of fewer operation steps, sensitive analysis results, and easy popularization of equipment.

Active Publication Date: 2012-06-27
QINGDAO HIGHTOP BIOTECH
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Problems solved by technology

The predecessors used indirect competitive chemiluminescent enzyme immunoassay to detect CL in pig urine samples, and the detection sensitivities were 0.08 μg / L and 0.01 μg / L, but the method was limited to the determination of CL in pig urine samples; some used indirect Determination of salbutamol and CL by competitive chemiluminescence enzyme immunoassay, but the operation is cumbersome and time-consuming

Method used

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  • Clenbuterol hydrochloride assay kit and its preparation method and use method
  • Clenbuterol hydrochloride assay kit and its preparation method and use method
  • Clenbuterol hydrochloride assay kit and its preparation method and use method

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preparation example Construction

[0069] 2. The preparation method of the above-mentioned clenbuterol hydrochloride detection kit, comprising:

[0070] 2.1 Preparation of clenbuterol hydrochloride microtiter plate

[0071] 2.1.1 Preparation of coating solution: use 0.1mol / L carbonate buffer solution (for example formula: 0.34g Na 2 CO 3 + 0.57g Na 2 HCO 3 , dilute to 100ml) dilute the 3mg / ml clenbuterol hydrochloride antibody solution to 2μg / ml;

[0072] 2.1.2 Coating amount: Add 100 μl of the coating solution prepared above to each well of the enzyme-labeled plate, and coat at 4°C for 48 hours;

[0073] 2.1.3 Washing: Wash the above-mentioned coated ELISA plate twice with the concentrated washing solution, the method is to dilute the concentrated washing solution at 1:40, that is, 1 volume of concentrated washing solution + 39 volumes of distilled water, with an interval of 0 seconds, at Pat dry on clean gauze or absorbent paper;

[0074] 2.1.4 Blocking: Add 130 μl of blocking solution to each well of ...

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Abstract

The invention discloses a clenbuterol hydrochloride assay kit and its preparation method and use method. The clenbuterol hydrochloride assay kit comprises a kit body, a clenbuterol hydrochloride enzyme label plate, a clenbuterol hydrochloride standard solution, a clenbuterol hydrochloride enzyme conjugate, a diluent, a concentrated washing liquid, a first substrate and a second substrate. The preparation method provided by the invention comprises preparing the clenbuterol hydrochloride enzyme label plate, the clenbuterol hydrochloride standard solution, the clenbuterol hydrochloride enzyme conjugate, the diluent, the concentrated washing liquid, the first substrate and the second substrate. The use method provided by the invention comprises sample preparation, sample addition and detection, and detection result analysis. The clenbuterol hydrochloride assay kit has the advantages that sensitive, stable and reliable result analysis is realized; operation steps are less; sample pre-treatment is simple; adopted equipment can be popularized easily; and the clenbuterol hydrochloride assay kit can be utilized for determination of residual clenbuterol hydrochloride of pork, has sensitivity of 0.03 micrograms per kilogram, needs detection time shorter than detection time of an ELISA method by 30 minutes and satisfies requirements of rapid detection of clenbuterol hydrochloride residues.

Description

technical field [0001] The present invention relates to a clenbuterol hydrochloride detection kit and its preparation and use method, in particular to a clenbuterol hydrochloride detection kit using direct competitive chemiluminescence immunoassay and its preparation and use method, belonging to Clenbuterol testing field. Background technique [0002] Clenbuterol hydrochloride (elenbuterol hydroehloride, referred to as CL) is a β 2 -Adrenergic receptor agonists, the trade names are bromide, clenbuterol, a derivative of catecholamines, commonly known as "clenbuterol". Adding a certain amount of CL to feed can significantly promote animal growth rate and feed conversion rate, promote animal fat decomposition, and increase lean meat rate by about 10%. With the application of CL in animal husbandry, it was found that CL can accumulate residues in animals, leading to food poisoning. CL poisoning can cause serious harm to human health, and its symptoms include: increased blood ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543G01N33/531G01N33/535
Inventor 杨致亭孙明强陶德友管廷武刘发新
Owner QINGDAO HIGHTOP BIOTECH
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