SERS detection method of brodifacoum or bromadiolone in edible oil

A detection method, edible oil technology, applied in the field of analysis and detection, can solve the problems of long sample detection time, high detection cost, cumbersome process, etc., and achieve the effect of simple operation, high detection accuracy and short time

Pending Publication Date: 2020-04-24
安徽中科赛飞尔科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The whole process includes sample pretreatment and large-scale instrument detection. The process is relatively cumbersome, the sample detection time is long, and the detection cost is high.

Method used

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  • SERS detection method of brodifacoum or bromadiolone in edible oil
  • SERS detection method of brodifacoum or bromadiolone in edible oil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A kind of SERS detection method of bromadiolone in edible oil, comprises the steps:

[0026] (1) Use a pipette to draw 0.6mL of edible oil samples containing bromadiolone (the concentration of bromadiolone is 2ppm), put them into a 5mL centrifuge tube, and then use a pipette to draw 0.6mL of ethyl acetate / chloroform (8:1), add to the centrifuge tube, then fully shake and mix with a vortex shaker for 1 minute;

[0027] (2) Add 3.2 mL of prepared 35°C saturated NaCO to the well-mixed centrifuge tube 3 solution. Then use a vortex shaker to fully shake and mix again for 1 minute; after fully mixing, use a centrifuge to centrifuge at a rate of 8000r / min for 3min; after centrifugation, take 7uL supernatant;

[0028] (3) 7uL supernatant is added dropwise to the silver nanocube base, and the above-mentioned silver nanocube base with the supernatant is irradiated with a handheld Raman spectrometer with an excitation wavelength of 532nm. After the detection is completed, bromad...

Embodiment 2

[0031] A kind of SERS detection method of bromadiolone in edible oil, comprises the steps:

[0032] (1) Use a pipette to draw 0.4mL of the edible oil sample containing bromadiolone (the concentration of bromadiolone is 0.5ppm), put it into a 5mL centrifuge tube, and then use a pipette to draw 0.8mL of ethyl acetate / Chloroform (8:1), was added to the centrifuge tube, then fully oscillated and mixed with a vortex shaker for 1 minute;

[0033] (2) Add 3.3 mL of prepared 50°C saturated Na to the well-mixed centrifuge tube 2 CO 3 solution. Then use a vortex shaker to oscillate and mix again for 1 minute; after fully mixing, use a centrifuge to centrifuge at a rate of 10000r / min for 2min; after centrifugation, take 10uL of supernatant;

[0034] (3) 10uL supernatant is added dropwise to the silver nanocube base, and the above-mentioned silver nanocube base with the supernatant is irradiated with a handheld Raman spectrometer with an excitation light wavelength of 532nm. After the...

Embodiment 3

[0037] A kind of SERS detection method of bromadiolone in edible oil, comprises the steps:

[0038] (1) Use a pipette to draw 0.7mL of the edible oil sample containing Dalong (the concentration of Dalong is 0.1ppm), put it into a 5mL centrifuge tube, and then use a pipette to draw 0.5mL of ethyl acetate / chloroform ( 8:1), added to the centrifuge tube, then fully oscillated and mixed with a vortex shaker for 1 minute;

[0039] (2) Add 3.1 mL of prepared 30°C saturated Na to the well-mixed centrifuge tube 2 CO 3 solution. Then use a vortex shaker to fully shake and mix again for 1 minute; after fully mixing, use a centrifuge to centrifuge at a rate of 8000r / min for 3min; after centrifugation, take 7uL supernatant;

[0040](3) Add 7uL of the supernatant to the silver nanocube substrate dropwise, and irradiate the above-mentioned silver nanocube substrate with the supernatant dripped with a handheld Raman spectrometer with an excitation wavelength of 532nm. After the detection ...

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Abstract

The invention provides an SERS detection method of brodifacoum or bromadiolone in edible oil. The method comprises the following steps: mixing the edible oil containing the brodifacoum or bromadiolonewith a mixed solution of ethyl acetate and chloroform, adding a saturated Na2CO3 water solution, carrying out mixing, and carrying out centrifugal separation to obtain a supernatant; and dropwise adding the supernatant to a substrate of the silver nanocube, carrying out detecting by using a Raman spectrometer, and acquiring and obtaining SERS signals of the brodifacoum or bromadiolone. The detection method has the advantages of accurate detection result, rapid detection and the like.

Description

technical field [0001] The invention relates to the technical field of analysis and detection, in particular to a SERS detection method for Dalong or Bromadiolone in edible oil. Background technique [0002] Dalong and bromadiolone are high-efficiency rodenticides with good palatability, high toxicity and wide target spectrum. If it is taken by mistake, the patient often manifests as body bruises, blood in the stool, and blood in the urine. As widely used rodenticides, Dalong and Bromadiolone are relatively easy to obtain and are often used as poisons by criminals. In recent years, cases of poisoning with Dalong and Bromadiolone have also been on the rise. [0003] At present, for the detection of Dalong and bromadiolone, the existing detection methods are widely used in biological samples, such as urine, blood, gastric solubles, and liver. Generally, for the detection of bromadiolone in complex matrices, large-scale instruments such as liquid chromatographs, gas chromato...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 杨良保袁伟林东岳张云峰
Owner 安徽中科赛飞尔科技有限公司
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