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Verifying test paper of sulfamerazine gel by gold method and production thereof

A coupling technology of sulfamethazine and dimethylpyrimidine, which is applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of long detection time, small amount of sample screening, high detection cost, etc., and meet the requirements of fast Detection effect

Inactive Publication Date: 2006-08-16
YUNNAN UNIV +2
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  • Abstract
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Problems solved by technology

At present, the methods for determining sulfamethazine mainly include methods such as high-performance liquid chromatography (HPLC) and enzyme-linked immunosorbent immunoassay. The HPLC method requires special instruments and equipment and professional technicians, and the amount of sample screening is small, and pretreatment of the sample is required. , so the detection cost is high and the detection time is long. These factors limit the development of the HPLC method for the detection of sulfamethazine, and cannot fully meet the needs of current food safety for the detection of sulfamethazine residues. Reagents mainly rely on imports, and the cost of testing is relatively high

Method used

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  • Verifying test paper of sulfamerazine gel by gold method and production thereof
  • Verifying test paper of sulfamerazine gel by gold method and production thereof
  • Verifying test paper of sulfamerazine gel by gold method and production thereof

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Experimental program
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Embodiment Construction

[0023] 1. Sulfamethazine detection test paper

[0024] On the front from bottom to top are lower absorbent pad 1, gold standard sulfamethazine antibody 2, sulfamethazine conjugated antigen 3, anti-mouse IgG antibody 4, upper absorbent pad 5, and nitrocellulose membrane 6, Plastic liner7.

[0025] The surface of the lower absorbent pad 1 and the solid-phase gold-labeled monoclonal antibody 2 and the surface of the upper absorbent pad 5 are pasted with colored self-adhesive paper.

[0026] Among them, the sulfamethazine-coupled antigen 3 was used as the detection band, and the anti-mouse IgG antibody 4 was used as the reference band.

[0027] The width of the membrane strip is not less than 2.5m, and the appearance is flat, the material is firmly attached, and the liquid migration speed is not less than 5mm / min.

[0028] Two, the preparation of sulfamethazine detection test paper

[0029] (1) Combining sulfamethazine with bovine serum albumin molecules to form sulfamethazine-...

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Abstract

A method for preparing test paper of sulfamethazine colloidal gold detection technique anchors sulfamethazine coupled antigen and resisting mouse IgG antibody as well as sulfamethazine single resistance being labeled with colloidal gold on nitrocellulose film .The prepared test paper can utilize one step competition process of chromatographic type to detect out residual amount of sulfamethazine in tested sample quickly and economically.

Description

Technical field [0001] The invention belongs to a test paper and a preparation method for testing an antigen by immunoreacting with a labeled antibody and the antigen. Background technique [0002] Sulfamethazine (Sulfamethazine) belongs to the sulfonamides and is an antibiotic with broad-spectrum antibacterial activity. It is used as an antibacterial agent in veterinary clinics to prevent or treat bacterial infectious diseases, and its sub-therapeutic concentration is used as a feed drug for livestock and poultry. Additives are used in animal husbandry production to control certain animal diseases, improve the conversion rate of feed and promote the growth and development of animals. Sulfamethazine is a suspected carcinogen, which can induce human thyroid cancer and produce certain drug resistance. People who are allergic to sulfamethazine can cause heart palpitations, shortness of breath and other adverse reactions. The resulting residues in fo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/544G01N33/558G01N33/52
Inventor 马岚袁航葛长荣张曦
Owner YUNNAN UNIV
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