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SERS (surface enhanced Raman scattering) detection method for trace toxic mice in vomit

A detection method and technology of vomit, which is applied in the field of analysis and detection, can solve the problems of difficulty in meeting analysis requirements, low sensitivity of chemical methods, difficulty in popularization and application, etc., and achieve the effects of short detection time, high sensitivity SERS effect, and improved sensitivity

Pending Publication Date: 2021-04-16
安徽中科赛飞尔科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The chemical method has low sensitivity, many interference factors, and is difficult to meet the analysis requirements; GC / MS is accurate in qualitative, high in sensitivity, and strong in anti-interference. popularization and application

Method used

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  • SERS (surface enhanced Raman scattering) detection method for trace toxic mice in vomit

Examples

Experimental program
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Effect test

Embodiment 1

[0025] A kind of SERS detection method of trace tetramine in vomitus, comprises the steps:

[0026] (1) Take 3mL of tetramine-containing vomit samples (the pre-simulated added tetramine concentration is 0.1ppm, that is, the sample contains tetramine 3×10 -7 mg) into a centrifuge tube, add 3g of anhydrous MgSO 4 Mix evenly; then use a pipette gun to draw 3mL of butyl acetate into the centrifuge tube, then use a vortex shaker to fully shake and mix for 2 minutes, use a centrifuge to centrifuge at a speed of 8000r / min for 3min, and take out the supernatant; Use a pipette gun to draw 3mL butyl acetate into the centrifuge tube at a time, vortex shaker to fully shake and mix for 2 minutes, use a centrifuge to centrifuge at a speed of 8000r / min for 3min, and take out the supernatant; the supernatant Combined with the previous supernatant, the resulting mixed liquid nitrogen was blown and concentrated to 0.5mL, which was the liquid to be tested;

[0027] (2) Add 6uL of the above-men...

Embodiment 2

[0030] A kind of SERS detection method of trace tetramine in vomitus, comprises the steps:

[0031] (1) Take 4mL of tetramine-containing vomit samples and put them into a centrifuge tube, add 5g of anhydrous MgSO 4 Mix well; then use a pipette gun to draw 5.5mL of butyl acetate into the centrifuge tube, then use a vortex shaker to fully shake and mix for 1 minute, use a centrifuge to centrifuge at 8000r / min for 3 minutes, and take out the supernatant ; Draw 4.5 mL of butyl acetate into the centrifuge tube again with a pipette gun, fully oscillate and mix with a vortex shaker for 1 minute, centrifuge at a speed of 8000 r / min for 3 minutes with a centrifuge, and take out the supernatant; and Combine the supernatant with the previous supernatant, blow and concentrate the resulting mixed liquid to 0.5 mL, which is the liquid to be tested;

[0032] (2) Add and drop 5uL of the above-mentioned liquid to be detected on the SERS substrate of Ag nanoflowers, and use a Raman spectromete...

Embodiment 3

[0035] A kind of SERS detection method of trace tetramine in vomitus, comprises the steps:

[0036] (1) Take 5 mL of tetramine-containing vomit sample and put it into a centrifuge tube, add 4 g of anhydrous MgSO 4 Mix well; then use a pipette gun to draw 4mL of butyl acetate into the centrifuge tube, then use a vortex shaker to fully shake and mix for 2 minutes, use a centrifuge to centrifuge at 8000r / min for 3min, and take out the supernatant; Draw 3.5mL of butyl acetate into the centrifuge tube again with a pipette gun, fully oscillate and mix with a vortex oscillator for 2 minutes, centrifuge at a speed of 8000r / min for 3min with a centrifuge, take out the supernatant, and The supernatant is combined with the previous supernatant, and the resulting mixed liquid is blown with nitrogen and concentrated to 0.5 mL, which is the liquid to be tested;

[0037] (2) Add 7uL of the above-mentioned solution to be detected to the SERS substrate of Ag nanoflowers, and use a Raman spect...

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Abstract

The invention provides an SERS (Surface Enhanced Raman Scattering) detection method for trace toxic mouse strength in vomit, which comprises the following steps: mixing a vomit extracting solution containing toxic mouse strength with an SERS substrate, carrying out SERS detection by using a Raman spectrometer, and collecting and obtaining an SERS spectral signal of toxic mouse strength. According to the SERS detection method for trace toxic mouse strength in vomit provided by the invention, after a sample is pretreated, the sample is sampled and dripped on the SERS substrate for detection, and compared with traditional toxic mouse strength detection, the SERS detection method has the advantages of accurate detection result, short time, simple operation and the like.

Description

technical field [0001] The invention relates to the technical field of analysis and detection, in particular to a SERS detection method for trace tetramine in vomitus. Background technique [0002] Tetramine is also known as three-step down, no mouse life, rat king, mad cat and so on. Its chemical name is tetramethylene disulfone tetramine. It is a highly toxic acute central nervous excitatory rodenticide. Tetramine poisoning has the characteristics of small poisoning amount, fast toxic effect and serious consequences. In 1991, the state banned the production and use of tetramine, but it was banned repeatedly because of its remarkable rodent control effect and low price. As a result, poisoning incidents caused by accidental ingestion, environmental pollution, or human poisoning occurred frequently. [0003] The most direct sample involved in the poisoning incident is the vomitus of the poisoned person. The currently reported detection methods for tetramine include chemical...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65G01N1/28
Inventor 杨良保袁伟林东岳
Owner 安徽中科赛飞尔科技有限公司
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