The invention discloses a method for measuring content of oxygen, nitrogen and hydrogen in a titanium alloy. The method comprises the following steps: (1) performing treatment such as cutting and polishing on a high-purity electrolysis nickel board, thereby preparing a nickel particle fluxing agent; (2) putting the nickel particle fluxing agent into an inner high-purity graphite crucible of a lower electrode of an oxygen, nitrogen and hydrogen analyzer, and performing degassing treatment under high power; (3) selecting a measuring condition, and measuring oxygen, nitrogen and hydrogen gas in the titanium alloy through the oxygen, nitrogen and hydrogen analyzer; and (4) calculating mass percentage of oxygen, nitrogen and hydrogen in a test sample. According to the method disclosed by the invention, a self-made nickel particle fluxing agent with a relatively high blank value can be used to replace commercially available fluxing agents such as a nickel basket and nickel scraps, content of oxygen, nitrogen and hydrogen in the titanium alloy is measured through a pulse melting method, interference of the blank value can be remarkably reduced or eliminated by changing normal test steps and analysis conditions, and the same effects of the commercially available fluxing agents are achieved, so that detection cost is greatly reduced; and the method has the advantages of being simple, high in accuracy and the like, and meets the quick detecting requirements.