Preparation method and application of ratiometric double fluorescence probe

A dual-fluorescence and ratiometric technology, which is applied in the field of preparation of ratiometric dual-fluorescence probes, can solve problems such as cell environment imbalance and biological organ toxicity, and achieve the effects of good selectivity, good stability, and avoidance of leakage.

Inactive Publication Date: 2015-12-16
TAIYUAN UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, excessive copper ions can lead to an imbalance in the cellular environment and cause severe toxicity to biological organs

Method used

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  • Preparation method and application of ratiometric double fluorescence probe
  • Preparation method and application of ratiometric double fluorescence probe
  • Preparation method and application of ratiometric double fluorescence probe

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Embodiment 1

[0044] A method for preparing a dual-fluorescence ratiometric fluorescent probe for quantitative determination of copper ions by covalently linking bovine serum albumin-stabilized gold nanoclusters (AuBSANCs) to amino-modified CdTeSiO 2 On the surface of microspheres, CdTeSiO 2 AuNCs hybrid microspheres, and develop them to achieve Cu 2+ A ratiometric fluorescent probe for quantitative determination, comprising the steps of:

[0045] 1) Preparation of CdTe quantum dots

[0046] Mix sodium borohydride and tellurium powder at a molar ratio of 5:1, and react under nitrogen atmosphere to prepare sodium telluride hydride (NaHTe) precursor; take 2ml of the fresh precursor solution and add it to Mixed solution of cadmium chloride and mercaptopropionic acid saturated with nitrogen (mercaptopropionic acid and CdCl 2 The molar ratio is 2.4:1), and under the condition of pH = 9.0, the reaction was heated at 100 °C for 4 h to generate CdTeQDs with an emission wavelength of 550 nm. Not...

Embodiment 2

[0063] A method for preparing a dual-fluorescence ratiometric fluorescent probe for quantitative determination of copper ions by covalently linking bovine serum albumin-stabilized gold nanoclusters (AuBSANCs) to amino-modified CdTeSiO 2 On the surface of microspheres, CdTeSiO 2 AuNCs hybrid microspheres, including the following steps:

[0064] 1) Preparation of CdTe quantum dots

[0065] Mix sodium borohydride and tellurium powder at a molar ratio of 6:1, and react under nitrogen atmosphere to prepare sodium telluride hydride (NaHTe) precursor; take 2ml of the fresh precursor solution and add it to Mixed solution of cadmium chloride and mercaptopropionic acid saturated with nitrogen (mercaptopropionic acid and CdCl 2 The molar ratio is 2:1), and under the condition of pH = 9.0, the reaction was heated at 100 °C for 3 h to generate CdTeQDs with an emission wavelength of 540 nm.

[0066] 2) CdTeSiO 2 preparation of

[0067] 6 mL of fresh CdTeQDs solution was added to 40 mL ...

Embodiment 3

[0075] A method for preparing a dual-fluorescence ratiometric fluorescent probe for quantitative determination of copper ions by covalently linking bovine serum albumin-stabilized gold nanoclusters (AuBSANCs) to amino-modified CdTeSiO 2 On the surface of microspheres, CdTeSiO 2 AuNCs hybrid microspheres, including the following steps:

[0076] 1) Preparation of CdTe quantum dots

[0077] Mix sodium borohydride and tellurium powder at a molar ratio of 4:1, and react under a nitrogen atmosphere to prepare a sodium telluride hydride (NaHTe) precursor; take 2ml of the fresh precursor solution and add it to Mixed solution of cadmium chloride and mercaptopropionic acid saturated with nitrogen (mercaptopropionic acid and CdCl 2 The molar ratio is 6:1), and under the condition of pH = 10.0, the reaction was heated at 100 °C for 5 h to generate CdTeQDs with an emission wavelength of 560 nm.

[0078] 2) CdTeSiO 2 preparation of

[0079] 5 mL of fresh CdTeQDs solution was added to 3...

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Abstract

The invention discloses a preparation method and application of a ratiometric double fluorescence probe. CdTe quantum dot and Au nano-cluster are taken as a fluorescence signal source, a silicon-based material is taken as a carrier, the quantum dot is taken as a reference signal source and is embedded in a silicon core, and the Au NCs (Au nano-cluster) with fluorescence response performance is covalently bonded to the surface of a silicon layer, so that a hybrid microballoon with the core-satellite type structure is prepared. The advantages comprise that the quantum dot is taken as the reference signal source and is embedded into the silicon core, leakage is effectively avoided, a stable reference signal is provided; and by covalently bonding the Au NCs with the fluorescence response performance to the silicon ball surface, selective identification and response of Cu<2+> are realized.

Description

technical field [0001] The invention relates to a preparation method and application of a ratio-type dual fluorescent probe, in particular to CdTeSiO with a core-satellite structure 2 Preparation method of AuNCs hybrid fluorescent probe and its application on Cu 2+ Applications in Quantitative Determination. Background technique [0002] Compared with single fluorescence quenching fluorescent probes, the signal changes of ratiometric fluorescent probes are easier to be observed by the naked eye. (WangHH, XueL, QianYY, etal, Orglett, 2010, 12:292~295) . The ratio-type fluorescence method uses the ratio of the fluorescence intensity at two wavelengths with good resolution as the signal, so that it can provide internal correction for the influence caused by other factors in the environment except the analyte, and eliminate the intensity drift caused by the excitation light source. The signal change has nothing to do with the concentration of the sensor itself, so the accurac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/88C09K11/02G01N21/64
Inventor 王艳芹武晓刚武晓红王颖王兆伟赵彤李文友韩志军陈维毅
Owner TAIYUAN UNIV OF TECH
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