Planar miniature multi-channel fluorescence detection optical system

Abstract

The invention discloses a planar miniature multi-channel fluorescence detection optical system which comprises a planar exciting light assembly and a planar lighting assembly; the reaction tank is respectively connected to said two assemblies through optical fibers; the planar exciting light assembly includes that single LED light source light serves as single-channel light to penetrate through adichroscope after being filtered and collimated and / or is focused to an optical fiber after being reflected by the dichroscope; the single-channel light in different directions passes through the first-stage dichroscope and then is output as a group of light beams in the same emitting direction; the light beams in different directions pass through a second-stage dichroscope and then are output asa multi-channel light beam group in the same emitting direction; the planar lighting assembly is characterized in that a fluorescence reaction passes through a cylindrical lens and then penetrates through a dichroscope and / or is reflected by the dichroscope and then is separated into a plurality of single-channel fluorescence in different directions, and the single-channel fluorescence is focusedand filtered and then is transmitted to a photosensitive surface of a photodiode; the fluorescence in the single direction passes through a second-stage dichroscope and then is output as fluorescencebeams in different emitting directions; and each group of fluorescence beams pass through the first-stage dichroscope and then are output as single-channel fluorescence in different emitting directions.

Description

technical field [0001] The invention relates to the technical field of multi-channel fluorescence detection, in particular to a planar miniature multi-channel fluorescence detection optical system. Background technique [0002] Fluorescence detection is a natural luminescent reaction. Through the reaction of luciferase and ATP, it can detect human cells, bacteria, mold, and food residues, and the reaction results can be obtained within 15 seconds. Fluorescence illuminance is generally measured by light-sensing equipment and expressed in digital form. [0003] At present, fluorescence detection is the most widely used fluorescent quantitative PCR technology, which refers to the method of adding fluorescent groups to the PCR reaction system, realizing the real-time monitoring of the whole process of PCR through the continuous accumulation of fluorescent signals, and then quantitatively analyzing the unknown template through the standard curve. . [0004] Usually, in fluoresc...

AI Technical Summary

Problems solved by technology

[0005] The technical solution of the present invention is: a planar miniature multi-channel fluorescence detection optical system, which can be applied to fluorescence quantitative PCR, portable PCR, fast PCR, etc., and solves the problem that the traditional fluorescence detection optical system is bulky and the three-dimensional type is not good in equipment. Placement, the problem of poor installation of optical components

At the same time, it also solves the problem that the traditional multi-channel fluorescence detection optical system needs to use a mechanical turntable.

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