4 results about "Fast pcr" patented technology

The invention relates to a DNA polymerase and application thereof. Wherein the DNA polymerase is 1) a protein with an amino acid sequence as shown in SEQ ID NO: 1 or SEQ ID NO: 2; or 2) a protein which has an amino acid sequence having more than 80%, more preferably more than 90%, and more preferably more than 95% homology with the amino acid sequence as shown in SEQ ID NO: 1 or 2 and has DNA polymerase activity. The DNA polymerase has the advantages of high amplification rate, good thermal stability, 5 '-3' polymerization activity and 3 '-5' excision activity, DNA amplification can be carried out at a fast PCR extension rate during DNA amplification, market requirements can be well met, and the DNA polymerase has great application potential.

This invention belongs to the field of molecular detection technology and discloses a portable ultra-fast PCR detection system and its applications. The PCR detection system provided by this invention includes a sample pipette and a PCR amplification device, which together form an organic whole. Working in synergy, they can effectively achieve rapid detection of target nucleic acids in complex clinical samples such as sputum and blood without extraction, with high specificity, high sensitivity, and high accuracy. It has excellent application prospects in the detection of pathogens for non-diagnostic purposes.

The utility model discloses an ultrafast PCR (Polymerase Chain Reaction) detection tube convenient to operate, and relates to the technical field of molecular biological detection consumables, the ultrafast PCR detection tube comprises an inner tube and a positioning component, the outer side of the inner tube is connected with an outer tube in a sliding manner, the positioning component is arranged above the inner tube, and the positioning component comprises a top ring, a rotating shaft, a torsion spring, a rotating plate, a clamping plate and a fixing ring, the interiors of the two ends of the top ring are rotationally connected with rotating shafts, and the outer sides of one ends of the rotating shafts are sleeved with torsional springs. According to the ultrafast PCR detection tube convenient to operate, through the arrangement of a positioning assembly, when an inner tube is inserted into an outer tube, an inclined plane on a fixed ring is in contact with an inclined plane at the bottom of a clamping plate, so that the clamping plate automatically moves outwards, and after insertion, a torsional spring drives a rotating plate to rotate, so that the clamping plate moves to the bottom of the fixed ring, and the inner tube is inserted into the outer tube; and the inner pipe and the outer pipe are automatically locked and fixed, so that the sealing performance of the detection pipe is prevented from being influenced by accidental loosening.