71 results about "Fast pcr" patented technology

A mobile rapid test system for nucleic acid analysis. A method comprising the steps of amplification of the nucleic acids by means of rapid-PCR technology, conversion of a double-stranded amplification product into a single-stranded DNA fragment, hybridization with a labeled probe and detection of the nucleic acids on a lateral-flow test strip. A device comprising a reaction cavity which preferably consists of a thin film, inlet and outlet openings for the reaction cavity, one or more heatable sample blocks which are connected to miniaturized cooling bodies and a window for reading off the result. The lateral-flow test strip is a component of the mobile rapid test system. Operation of the instrument system requires no external power source, but only batteries or a rechargeable battery.

The present invention provides mutants of DNA polymerases having an increased rate of incorporation of nucleotides into nucleic acids undergoing polymerization and having an enhanced resistance to inhibitors of DNA polymerase activity. The mutant polymerases are well suited for fast PCR applications, for PCR amplification of targets in samples that contain inhibitors of wild-type polymerases, and for fast PCR amplification of samples containing DNA polymerase inhibitors. In exemplary embodiments, the mutants are mutants of Taq DNA polymerase.

The present invention is specific SCAR label and specific primer of soybean cyst roundworm and their quick PCR detection method, and belongs to the field of biotechnology. The specific SCAR label of soybean cyst roundworm features its nucleotide sequence as shown in SEQ No. 1. The specific primers SCNF1 and SCNR1 designed based on the specific SCAR label of soybean cyst roundworm may be used in the quick PCR detection of soybean cyst roundworm and can amplify a 500 bp segment. It is also possible to add primers D2A and D3B as internal standards to amplify a 800 bp segment simultaneously. The present invention has raised molecular detection sensitivity, fast detection speed, accurate detection result and very high application value in the early detection of soybean cyst roundworm and the early diagnosis of soybean cyst roundworm disease.

The invention discloses a specific sequence characterized amplified region (SCAR) marker and a rapid polymerase chain reaction (PCR) detection method for Heterodera filipjevi, and belongs to the field of biotechnology. A deoxyribonucleic acid (DNA) fragment of the specific SCAR marker for the Heterodera filipjevi is characterized in that: the DNA fragment has a nucleotide sequence shown as SEQ ID NO1. Primers, namely HfF1 and HfR1 which are designed according to the DNA fragment of the specific SCAR marker for the Heterodera filipjevi can amplify a fragment of 313bp in the rapid PCR detection method for the Heterodera filipjevi; meanwhile, universal primers, namely TW81 and AB28 can also be added into a PCR reaction system to serve as interior labels, and a fragment of 1000bp can be synchronously amplified. The sensitivity of molecular detection is improved, and the method is quick and accurate, and has high application value in the fields of the detection of the Heterodera filipjevi and the early diagnosis of Heterodera filipjevi disease.

A microplate for polymerase chain reaction (PCR) comprises a substrate having a metallic material for heating PCR samples, and a barrier layer disposed adjacent to the substrate. In some cases, the barrier layer is formed of a first polymeric material. The microplate includes one or more wells for containing PCR samples. The one or more wells are formed of a second polymeric material sealed to the barrier layer. In some cases, the substrate can provide a PCR ramp rate of at least about 5° C./second.

The invention belongs to the field of molecular biology, and relates to a pair of phenacoccus solenopsis specific SS-COI primers, a rapid PCR detection method, and a kit. The primers, the method and the kit are suitable to be used for detecting phenacoccus solenopsis. According to the invention, according to specific mitochondrial DNA sequence of phenacoccus solenopsis, a pair of specific primers is designed. The primers only have amplification capacities upon phenacoccus solenopsis, wherein an amplification product size is 546bp. The primers also have good detection method upon single egg and new nymph. The pair of primers is a supplementation and improvement to a phenacoccus solenopsis mtDNA COI technical detection method. Also, an SS-COI PCR technology is adopted, such that detection accuracy is improved, and detection time is saved. The primers and the method can be popularized in a form of a kit in our ports, organic vegetable and organic fruit production base, cut flower production base, and transportations of vegetables, ornamental plants, and fruit trees seedlings/plants.

The invention belongs to the field of molecular biology, and relates to a pair of tuta absoluta specific SS-COI primers, a rapid PCR detection method, and a kit. According to the invention, according to specific mitochondrial DNA sequence of tuta absoluta, a pair of specific primers is designed. The primers only have amplification capacities upon tuta absoluta, wherein an amplification product size is 256bp. The primers also have good detection method upon single egg and adult residues. The pair of primers is a supplementation and improvement to tuta absoluta RAPD and rDNA ITS, and mtDNA COI technical detection methods. Also, an SS-COI PCR technology is adopted, such that detection accuracy is improved, and detection time is saved. The primers and the method can be popularized in our ports in a form of a kit.

The invention discloses a quick PCR (polymerase chain reaction) amplifier temperature control method and relates to the field of DNA amplification. The method includes that a heating mechanism at an air incoming position of an air drum in a fan component is started for heating, air heated by the heating mechanism is blown by the air drum to flow inside a shell and form a heat circulating air field, and a PCR pipe is heated by the air in the heat circulating air field; during cooling, the fan component outputs air, an air incoming door and an air outgoing door are opened, and cold air outside the shell enters the shell from the air incoming door and then is blown by the fan component to circularly flow inside the shell and then discharged from the air outgoing door. By using the method, heating and cooling rate of a PCR amplifier is increased, PCR amplification time is shortened effectively, and PCR amplification efficiency is high; probability that miscellaneous bands appear in experiments can be reduced, improving of experiment result accuracy is facilitated, and safety coefficient is high.

The invention provides a tigecycline resistance gene family tetX multiplex PCR detection kit. The tigecycline resistance gene family tetX multiplex PCR detection kit consists of a rapid PCR mixed solution, ultrapure water, a primer probe mixed solution, a standard substance and a negative reference substance, wherein the primer probe mixed solution comprises primer pairs of tetX1, tetX2, tetX3, tetX4, tetX5 and the like. According to the invention, a sensitive and efficient PCR detection method is established by using multiplex PCR test means, and strains containing tetX1, tetX2, tetX3, tetX4 and tetX5 genes can be rapidly and effectively detected. The tigecycline resistance gene family tetX multiplex PCR detection kit has the advantages of low cost, high efficiency, convenience in operation, high accuracy, less energy consumption, less environmental pollution and the like.

The invention discloses a primer pair for identifying antelope horn and application thereof. The primer pair for identifying or auxiliarily identifying antelope horn is particularly a primer pair composed of two single-chain DNA molecules disclosed as Sequence 1 and Sequence 2 in the sequence table. The experiment proves that the primers implement quick and accurate identification on antelope horn, mongolian gazelle horn, goa horn, Procapra przewalskii horn, goitered gazelle horn, goat horn and the like by quick PCR (polymerase chain reaction) and fluorescent dyeing. The fluorescent dyeing process is introduced to detect the identification result, thereby providing technical support for field application of drug molecule identification.

The invention discloses a primer pair used for identifying peucedanum praeruptorum and application thereof. The primer pair used for identifying peucedanum praeruptorum or assisting the identification of peucedanum praeruptorum is specifically a primer pair formed by two single-chain DNA molecules shown in a sequence 1 and a sequence 2 in a sequence table (shown in the description). An experiment result proves that through the adoption of the primer pair provided by the invention, the quick and accurate identification between the peucedanum praeruptorum and counterfeit species like peucedanum decursivum is realized through a quick PCR method, and technical support is provided for the site utilization of medicinal material molecule identification.

The present specification discloses methods of detecting a contaminant of interest in a sample, components useful in carrying out these methods, including PCR primers and a detection solution and kits thereof.

The invention discloses an SCAR markerer of a sorghum head smut resistance germ No. 3 physiological strain, which takes 7050B which is immune to head smut, Tx622B which is high sensitive to the head smut and F2 hybridized by Tx622B/7050B as test pieces and adopts a BSA method to make a molecular marker on a sorghum head smut resistance gene by applying an RAPD (Random Amplified Polymorphic DNA) screening technology. The invention establishes and optimizes a sorghum RAPD reaction system to obtain three RAPD markers of S18799, S336-11419 and S336-2716 which are tightly interlocked with the sorghum head smut resistance gene, also converts the S18799, the S336-11419 and the S336-2716 to stable SCAR markerers and uses the markers to carry out rapid PCR (Polymerase Chain Reaction) detection on the F2 generation and a part of sorghum resistant varieties and finds out a molecular marker specific band from the resistant varieties, thereby establishing an SCAR markerer rapid PCR detection method of the sorghum head smut resistance germ No. 3 physiological strain, supplying a basis for molecular marker assisted breeding and providing the technical support for practicing the molecular marker assisted breeding and shortening the breeding period.

The invention discloses a rapid PCR chip and a rapid fluorescent quantitative detector. The PCR chip comprises a panel and a bottom film, wherein at least one reaction tank is formed in the bottom surface of the panel; the reaction tank does not penetrate through the panel; the two sides of the reaction tank are provided with a sample adding hole and an exhaust hole and communicate with the top surface of the panel; the bottom film is attached to the bottom surface of the panel; and the panel is made of a light-transmitting material. When the PCR chip is arranged on a temperature control module, the bottom film is tightly attached to the temperature control module; a PCR solution in the reaction tank above the bottom film after sample adding is separated from the temperature control moduleby only a layer of bottom film; the refrigerating capacity or the heating capacity of the temperature control module can rapidly penetrate through the bottom film to be conducted to the PCR solution;and compared with a plastic plate, the film is higher in heat conduction speed and more uniform in heat conduction, so that rapid heating or cooling of the PCR solution is realized, and rapid PCR canbe realized. A heating and refrigerating sheet of the rapid fluorescent quantitative detector is horizontally placed; the PCR chip is horizontally placed on the heating and refrigerating sheet; the bottom film is tightly attached to the plane of the heating and refrigerating sheet; and the refrigerating capacity or the heating capacity of the heating and refrigerating sheet can be rapidly and uniformly conducted to the PCR solution.

The invention relates to a phenacoccus manihoti matile-ferrero specificity SS-COI primer pair, as well as a method and kit for quick PCR detection. The primer pair comprises primer PMZWMEF1:5'-CTTGATAAAACAGGAATTGAG-3' and primer PMZWMER1:5'-CCTTTGATGATTTCTTCTTCT-3'. The primer has amplification capacity just to the phenacoccus manihoti matile-ferrero, has favorable detection effect on eggs at the beginning of nympha and imago residue, and is supplement and modification for morphological detection identification method for the phenacoccus manihoti matile-ferrero; moreover, the method adopts the SS-COI PCR technology, improves accuracy of the detection, saves detection time, and can be popularized in a kit form in ports, cassava production bases, organic vegetable and fruit production bases, fresh and cut flower production bases, cotton planting zones, and transportation of vegetables, adornment plants, fruit germchits/plants.

The invention discloses a rapid nano-PCR detecting method of thermotolerant acid-resistant bacteria, which comprises the following steps: (1)separating thermotolerant acidophilic bacteria from the concentrated juice; (2)extracting the whole genome from thermotolerant acidophilic bacteria; (3)allocating rapid PCR system depended on nanometer silver; (4)setting and operating the rapid PCR condition; (5)detecting the PCR product with total time at 7.5h. The invention can monitor the thermotolerant acid-resistant bacteria of the condensed juice in the industrializing manufacturing effectively, which enlarges the directly economic benefit of export trade.

The invention relates to the field of molecular biology, and in particular relates to a pair of Paraleyrodes pseudonaranjae Martin specific SS-COI primers, a rapid PCR detection method and a kit. The Paraleyrodes pseudonaranjae Martin specific SS-COI primers comprise a primer PPZYF:5'-TAAAGGAACTAATCAATTTCCAAATCCC-3', and a primer PPZYR:5'-GGTCAACAAATCATAAAGATATTGGGT-3'. The primers PPZYF and PPZYR designed according to specific SS-COI mark of Paraleyrodes pseudonaranjae Martin can amplify segments of 233bp in the rapid PCR detection of Paraleyrodes pseudonaranjae Martin, and can not only detect single-head adult, two-age, three-age, and four-age nymph but also detect single spawn and single head newly-hatched nymph.

The invention provides a novel dual colorimetric sensing method based on a dual super-fast PCR. The method is used for Hg2+ and Ag+ visualization ultra-sensitive dual detection. The novel method comprises the steps that according to mispairing of mercury ion thymine, and mispairing of silver ion cytosine, a dual amplification primer and template of a super-fast polymerase chain reaction (PCR) aredesigned, meanwhile, the primer can be combined with an enzyme united nucleic acid self-assembly developing module, the novel dual target function nucleic acid detecting method and a biosensor based on mispaired mercury ions and silver ions are integrated and built. The built detection method and biosensor are faster and more sensitive, have the advantages of being high in specificity and sensitivity, reliable in detection result and the like compared with a traditional method, the analysis detection steps can be simplified, the analysis time can be shortened, more importantly, online real-time detection becomes possible, carrying and field operation are facilitated, and the method has the good application prospect in the field of rapid detection of heavy metals.

The invention relates to a primer library and screening system for rapid PCR (Polymerase Chain Reaction) detection for population sudden viral epidemics. The invention relates to a viral primer design and assessment system and belongs to the field of combination of technologies of biology and computer databases. The primer library and screening system mainly comprises a clinical virology database module, a virus sequence database module, a virus sequence automatic extraction module, a virus variation sequence function prediction module, a virus species specificity sequence and virus subtype specificity sequence screening module, a primer generation and evaluation module and an amplification product interpretation module. The invention aims to achieve the following purposes: firstly, an optimal PCR-amplified virus primer sequence is designed according to a target gene sequence; secondly, the specificity and accuracy of the primer are ensured; thirdly, the matching degree between an amplified fragment and a target virus is predicted under the condition that sequencing is not carried out; and fourthly, the clinical background data and referenced therapeutic schedules of a virus are predicted according to the amplified sequence of the PCR primer.

The present invention relates to a microfluidic system including a temperature controller and a thermoplastic microfluidic chip that enables rapid PCR in a PCR chamber of the microfluidic chip. Thermal control of the PCR chamber is achieved by applying voltage to heater electrodes patterned directly onto one layer of the microfluidic chip. The temperature controller adjusts the voltage applied to the heater electrodes by changing temperature controller parameters selected to minimize duration of each PCR cycle. Furthermore, simple operation of the microfluidic chip is provided through using an integrated passive capillary valve, requiring minimum operator intervention and eliminating the need for fluidic interfacing, pumping, or metering during chip loading.

The invention relates to a primer for detecting the sex of a single bull sperm or the purities of sperms X and Y in bull separation semen and application thereof. The primer comprises nucleotide sequences shown as SEQ ID NO.1 and 2. A method comprises the following steps of: performing amplification detection on the primer by a rapid polymerase chain reaction (PCR) method and identifying the sex of a single sperm through an agarose gel electrophoresis result according to a difference between the lengths of specific amplification products of two chromosomes; and performing single PCR amplification and agarose gel electrophoresis on each single bull sperm separated from the separation semen and performing statistical analysis on a single sperm sex detection result so as to make a final evaluation on the purity of the separation sperm. A specific PCR product can be amplified in any sperm cell by the method without designing any internal standard primer, the method is technology having the advantages of low cost, simple and convenient operation and rapid and correct identification of the separation purity of bull sperms X and Y and reliable and necessary technical support is provided for the popularization of subsequent control of semen and optimization and innovation of a sperm separation method.

The invention discloses a primer pair for identifying Scolopendra subspinipes multilans(L.)Koch and an application of the primer pair. The primer pair disclosed by the invention for identifying or assistant-identifying Scolopendra subspinipes multilans(L.)Koch is specifically the primer pair of two single-chain DNA molecules shown in sequences 1 and 2 in a sequence table. Experiments verify that by adopting the primers disclosed by the invention, Scolopendra subspinipes multilans(L.)Koch and Scolopendra multidens Newport are quickly and accurately identified by virtue of a quick PCR method and a fluorescent staining method and a fluorescent dye method is introduced to detect a true and false identification result, so that a technical support for field utilization on medicinal material molecular identification is realized.

The invention discloses a method for identifying dendrobium huoshanense by using a chloroplast microsatellite primer pair. The primer pair used for identifying dendrobium huoshanense or assisting identification of dendrobium huoshanense is a primer pair consisting of two single-chain DNA molecules as shown in sequence 1 and sequence 2 in a sequence table. Experiments prove that, by adopting the primer, the dendrobium huoshanense can be quickly and accurately identified from dendrobium wilsonii, dendrobium lohohense, dendrobium chrysotoxum, dendrobium thyrsiflorum, dendrobium lindleyi, dendrobium denndanum, dendrobium loddigesii, dendrobium dixanthum and other adulterants by adopting a quick PCR method, so that technical support is provided to field application of medicinal material molecular identification.

The invention discloses a primer pair for identifying a traditional Chinese medicine, namely, akebia stems, as well as an application of the primer pair. Theprimer pair for identifyingor assisting in identifying the traditional Chinese medicine, namely, the akebia stems, specifically consists of two single-stranded DNAmolecules represented in Sequence 1 and Sequence 2 in a sequence table. Tests prove that the traditional Chinese medicine, namely, the akebia stems, armand clematis stems and manchurian dutchmanspipe stems are identified quickly and accurately by the aid of the primer pair with a rapid PCR (polymerase chain reaction) method and a fluorescence staining method, authenticity identification results are detected with an introduced fluorescent dye method, and the technical support is provided for field application of medicine molecule identification.

The invention discloses a primer pair for identifying dendrobium huoshanense and dendrobium candidum. The primer pair consists of a primer SEQ ID No. 1 single-stranded DNA molecule and a primer SEQ IDNo. 2 single-stranded DNA molecule, wherein the SEQ ID No. 1 single-stranded DNA molecule is a single-stranded DNA molecule in a sequence 1 in a sequence table, and the SEQ ID No. 2 single-stranded DNA molecule is a single-stranded DNA molecule in a sequence 2 in the sequence table. According to the invention, through the specific primer pair, including the primer SEQ ID No. 1 single-stranded DNAmolecule and the primer SEQ ID No. 2 single-stranded DNA molecule, rapid and accurate identification between the dendrobium huoshanense and the dendrobium candidum is achieved by using a rapid PCR method, thereby providing a new method for identification of the dendrobium huoshanense.

The invention relates to an aleyrodes proletella specificity SS-COI primer pair, a rapid PCR detecting method and a kit. The aleyrodes proletella specificity SS-COI primer pair comprises a primer APZYJF: 5'-CAGCTTTATAATGTATTGGTCACA-3' and a primer APZYJR: 5'-CTCAAATTTTATACCCAACAAA-3'. According to the invention, the primers have amplification capability for aleyrodes proletella, and the detection sensitivity reaches that one female adult insect can be detected among 25600 female adult insects, which is the supplementation and improvement of the morphological detection and identification method of the aleyrodes proletella; in addition, the SS-COI PCR technology is adopted, so that the detection accuracy is improved, the detection time is saved, and the aleyrodes proletella specificity SS-COI primer pair can be popularized in national ports, organic vegetable production bases, fresh cut flower production bases and in the dispatching process of vegetables, ornamental plant-species and plants.