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Short peptide and application thereof

A technology of short peptides and amino acids, applied in the fields of biological science and drug carriers, to achieve the effects of enhanced selectivity, simple synthesis and reduced toxicity

Inactive Publication Date: 2011-08-17
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to research, cell-penetrating peptides can carry a variety of substances, including hydrophilic proteins, polypeptides, DNA, etc.

Method used

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  • Short peptide and application thereof
  • Short peptide and application thereof

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Embodiment Construction

[0023] The short peptide of the present invention is a short peptide composed of a cationic cell penetrating peptide, a connecting sequence and a targeting peptide sequence, and is synthesized by solid-phase chemistry. Membrane peptide plays the role of encapsulating plasmid DNA or other drugs, HHHH is the connecting sequence, which plays the role of connecting and eliminating steric hindrance, and LTVSPWY is the targeting peptide, which plays the role of enriching to the target cells and being taken up by them.

[0024] Observe the encapsulation of plasmid DNA by p20:

[0025] First, the plasmid DNA is wrapped with p20, N represents the positive charge of the peptide chain, and P represents the negative charge of the DNA. figure 1 This is the agarose gel electrophoresis picture of p20 and plasmid DNA mixed with different N / P ratios. It can be seen from the figure that the DNA has been basically completely bound since the N / P ratio is 3:1. figure 2 It is the Zeta potential m...

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Abstract

The invention discloses a short peptide and application thereof, belonging to the fields of biological science and medicinal carriers. The short peptide consists of a cationic cell penetrating peptide connecting sequence and a targeted peptide sequence, and the amino acid residue sequence of the short peptide is shown as SEQ ID NO.1 in a sequence table. The short peptide can be used for targeted transfection of tumor cells.

Description

technical field [0001] The invention belongs to the field of biological science and drug carrier. Background technique [0002] An important difficulty in gene therapy and other drug treatments at present is the lack of safe and effective carriers that can deliver drugs to specific locations and enter cells to play a role. To achieve this goal, taking gene therapy as an example, researchers have developed a variety of delivery vehicles. Due to the safety and operational complexity of viral vectors, research on non-viral vectors has also received greater attention. Common non-viral vectors include liposomes and cationic polymers. [0003] Liposome is currently one of the gene carriers with the highest delivery efficiency, has good stability to serum, and is easy to use. However, it is highly toxic. In vivo studies have found that it will accumulate in the lungs and cause inflammation, and the particles formed by wrapping with DNA have a large particle size (>500nm), whi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/08C12N15/63
Inventor 张玉慧骆清铭龚铖
Owner HUAZHONG UNIV OF SCI & TECH
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