Use of a traditional Chinese medicine composition in the preparation of a liver cancer resistant drug

Abstract

The application belongs to the technical field of traditional Chinese medicines, and particularly relates to application of a traditional Chinese medicine composition in preparation of a liver cancer resisting medicine. The traditional Chinese medicine composition comprises red ginseng, ox horn, musk, borneol, safflower, ox gall, appendices, toad venom, panax notoginseng, storax and pearl. Experimental results show that the traditional Chinese medicine composition can induce apoptosis and necrosis of liver cancer cells, inhibit proliferation of the liver cancer cells, and block the liver cancer cells in the G1 phase of the cell cycle. Therefore, the application provides a new treatment medicine for clinical treatment of liver cancer, and the raw materials are natural medicinal materials, are widely sourced, and have a good application prospect.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of traditional Chinese medicine, and particularly relates to application of a traditional Chinese medicine composition in preparation of an anti-liver cancer drug. BACKGROUND

[0002] Liver cancer is a highly malignant tumor that grows rapidly and has strong infiltration and metastasis ability, 75-90% of which is of hepatocellular carcinoma. It is generally found in liver metastasis of malignant tumors of organs such as stomach, biliary tract, pancreas, colorectum, ovary, uterus, lung and breast, and the etiology and exact molecular mechanism are not completely clear. It is considered that the onset is a complex process of multiple factors and multiple steps, and is affected by environmental and dietary factors.

[0003] At present, the treatment methods for liver cancer include resection, radiotherapy and chemotherapy, intervention, targeted drugs, immunotherapy, traditional Chinese medicine treatment, etc., and resection is the main treatment method. However, due to the high early concealment and rapid progression of liver cancer cells, most liver cancer patients are found to be in the middle and late stages. And the liver cancer cell lesions are multiple and easy to diffuse, so only less than 30% of liver cancer patients meet the liver cancer resection treatment. Anti-liver cancer drugs mainly include western medicine and traditional Chinese medicine. Western medicine: anti-liver cancer western medicine mainly includes sunitinib, erlotinib and cetuximab; traditional Chinese medicine: during the anti-cancer period, traditional Chinese medicine can also be used for conditioning, and common drugs include huacan oral liquid, shendan sanjie capsule and compound cantharidin capsule. However, these drugs do not have liver cancer targeting and have large side effects.

[0004] Therefore, it is of great significance to develop an anti-liver cancer traditional Chinese medicine with good anti-liver cancer effect and low side effect. SUMMARY

[0005] The application provides application of a traditional Chinese medicine composition in preparation of an anti-liver cancer drug.

[0006] To achieve the above object, the application adopts the following technical scheme:

[0007] The application provides application of a traditional Chinese medicine composition in preparation of an anti-liver cancer drug, wherein the traditional Chinese medicine composition comprises red ginseng, ox horn tip, musk, borneol, safflower, ox gall, appendage, toad venom, panax notoginseng, storax and pearl.

[0008] Preferably, the application of the traditional Chinese medicine composition in preparation of a drug for blocking liver cancer cells in the cell cycle G1 phase.

[0009] Preferably, the application of the traditional Chinese medicine composition in preparation of a drug for inducing apoptosis and necrosis of liver cancer cells.

[0010] Preferably, the liver cancer cells are HepG2 cells, SMMC-7721 cells or H22 cells.

[0011] Preferably, the traditional Chinese medicine composition comprises, by weight parts: red ginseng 80-90 parts, cowhorn tip 25-35 parts, musk 5.5-6.5 parts, borneol 17-18 parts, safflower 5.5-6.5 parts, ox gall 35-36 parts, appendage 35-36 parts, toad venom 14-15 parts, panax notoginseng 38-39 parts, storax 17-18 parts, and pearl 20-21 parts.

[0012] More preferably, the traditional Chinese medicine composition comprises, by weight parts: red ginseng 88.2 parts, cowhorn tip 29.4 parts, musk 5.9 parts, borneol 17.6 parts, safflower 5.9 parts, ox gall 35.3 parts, appendage 35.3 parts, toad venom 14.7 parts, panax notoginseng 38.2 parts, storax 17.6 parts, and pearl 20.6 parts.

[0013] Preferably, the preparation method of the traditional Chinese medicine composition comprises the following steps:

[0014] (1) First, panax notoginseng, pearl, cowhorn tip, borneol, musk and ox gall are crushed, mixed after sieving, to obtain a mixed powder;

[0015] (2) Then, red ginseng, appendage, safflower and toad venom are respectively immersed in an ethanol aqueous solution, percolation is performed, the percolate is collected, and concentration is performed to obtain four kinds of extract;

[0016] (3) Finally, the four kinds of extract are combined, the mixed powder and storax dissolved in an ethanol aqueous solution are added, and mixing is performed, and drying is performed, to obtain the traditional Chinese medicine composition.

[0017] Preferably, the mesh number of the sieving in step (1) is 90-110.

[0018] Preferably, the immersion time in step (2) is 24-36 h, the volume fraction of the ethanol aqueous solution is 50-70%, and the relative density of the extract at 50-60℃ is 1.25-1.35 g / mL.

[0019] Preferably, in step (2), the red ginseng is immersed in 7-9 times the amount of the ethanol aqueous solution; the appendage is immersed in 5-7 times the amount of the ethanol aqueous solution; the safflower is immersed in 19-21 times the amount of the ethanol aqueous solution; and the toad venom is immersed in 10-12 times the amount of the ethanol aqueous solution.

[0020] Preferably, in step (3), the extract after combination needs to be heated in a water bath for fusion, and the volume fraction of the ethanol aqueous solution is 70-80%.

[0021] Preferably, the medicine further comprises a pharmaceutically acceptable excipient, and the dosage form of the medicine is a tablet, a granule, a capsule, a pill or an oral liquid.

[0022] The pharmacological analysis of the raw material used in the present application is as follows:

[0023] Red ginseng: [Properties and Channels Entered] Sweet, slightly bitter, warm. Enters the spleen, lung, heart, and kidney channels. [Functions and Indications] Greatly replenishes vital energy, restores pulse and consolidates the body, benefits qi and controls bleeding. Used for weakness and impending collapse, cold limbs and weak pulse, qi failing to control blood, and metrorrhagia.

[0024] Ox Horn Tip: [Properties and Channels Entered] Bitter, cold. Enters the Heart and Liver channels. [Functions and Indications] Clears heat and cools blood, detoxifies, and calms the nerves. Used for febrile diseases with high fever, delirium, rashes, hematemesis, epistaxis, infantile convulsions, and mania.

[0025] Musk: [Properties and Channels Entered] Pungent, warm. Enters the Heart and Spleen channels. [Functions and Indications] Opens the orifices and awakens the mind, invigorates blood and unblocks menstruation, reduces swelling and relieves pain. Used for delirium due to febrile diseases, stroke with phlegm syncope, sudden syncope due to qi stagnation, coma due to sudden illness, amenorrhea, abdominal masses, difficult labor and stillbirth, chest pain and heart pain, sudden pain in the heart and abdomen, traumatic injuries, numbness and pain, carbuncles and scrofula, and sore throat.

[0026] Borneol: [Properties and Channels Entered] Pungent, bitter, slightly cold. Enters the Heart, Spleen, and Lung channels. [Functions and Indications] Opens the orifices and awakens the mind, clears heat and relieves pain. Used for delirium due to febrile diseases, convulsions, stroke with phlegm syncope, sudden syncope due to qi stagnation, coma due to sudden illness, chest pain, red eyes, oral ulcers, sore throat, and purulent discharge from the ear canal.

[0027] Safflower: [Properties and Channels Entered] Pungent, warm. Enters the Heart and Liver channels. [Functions and Indications] Activates blood circulation and regulates menstruation, disperses blood stasis and relieves pain. Used for amenorrhea, dysmenorrhea, lochia retention, abdominal masses, chest pain, abdominal pain due to blood stasis, stabbing pain in the chest and hypochondrium, traumatic injuries, and sores and swellings.

[0028] Calculus Bovis: [Properties and Channels Entered] Sweet, cool. Enters the Heart and Liver channels. [Functions and Indications] Clears the heart, resolves phlegm, opens the orifices, cools the liver, calms wind, and detoxifies. Used for delirium due to febrile disease, phlegm-induced coma due to stroke, convulsions, epilepsy, sore throat, mouth and tongue sores, carbuncles, boils, and furuncles.

[0029] Accompanying slices: Also known as black aconite slices.

Properties and Channels Entered

Functions and Indications

[0030] Toad venom: [Properties and Channels Entered] Pungent, warm; toxic. Enters the Heart Channel. [Functions and Indications] Detoxifies, relieves pain, clears the mind and refreshes the spirit. Used for carbuncles, boils, sore throat, heatstroke with delirium, abdominal distension, vomiting, and diarrhea.

[0031] Panax notoginseng: [Properties and Channels Entered] Sweet, slightly bitter, warm. Enters the Liver and Stomach channels. [Functions and Indications] Disperses blood stasis and stops bleeding, reduces swelling and relieves pain. Used for hemoptysis, hematemesis, epistaxis, hematochezia, metrorrhagia, traumatic bleeding, chest and abdominal pain, and swelling and pain from falls.

[0032] Benzoin: [Properties and Channels Entered] Pungent, bitter, neutral. Enters the Heart and Spleen channels. [Functions and Indications] Opens the orifices and awakens the mind, promotes qi and blood circulation, and relieves pain. Used for stroke with phlegm syncope, qi stagnation and sudden syncope, sudden coma due to illness, abdominal pain, postpartum hemorrhage, and infantile convulsions.

[0033] Pearl: [Properties and Channels Entered] Sweet, salty, cold. Enters the Heart and Liver channels. [Functions and Indications] Calms the mind and relieves fright, improves eyesight and removes corneal opacity, detoxifies and promotes tissue regeneration, moisturizes the skin and removes blemishes. Used for palpitations and insomnia, infantile convulsions and epilepsy, red eyes with corneal opacity, non-healing sores, and skin pigmentation.

[0034] Compared with the prior art, the present invention has the following beneficial effects:

[0035] This invention provides the use of a traditional Chinese medicine composition in the preparation of an anti-liver cancer drug. The raw materials of this composition include red ginseng, ox horn tip, musk, borneol, safflower, bezoar, aconite root, toad venom, Panax notoginseng, benzoin, and pearl. Experimental results show that this traditional Chinese medicine composition can induce apoptosis and necrosis of liver cancer cells, inhibit liver cancer cell proliferation, and arrest liver cancer cells in the G1 phase of the cell cycle. Therefore, this invention provides a new therapeutic drug for the clinical treatment of liver cancer, and the raw materials are natural medicinal materials with wide availability, showing good application prospects. Detailed Implementation

[0036] It is worth noting that all raw materials used in this invention are commercially available products. Specifically, HepG2 cells were purchased from Eddie Gene Technology Co., Ltd.; RPMI-1640 complete culture medium was purchased from Thermo Fisher Scientific; 5-fluorouracil, with a content ≥98%, was purchased from Sigma-Aldrich; and cisplatin was purchased from Texas Deyao Pharmaceutical Co., Ltd.

[0037] Example 1

[0038] A traditional Chinese medicine composition, by weight, comprises the following components: 88.2 parts red ginseng, 29.4 parts ox horn tip, 5.9 parts musk, 17.6 parts borneol, 5.9 parts safflower, 35.3 parts bezoar, 35.3 parts aconite root, 14.7 parts toad venom, 38.2 parts Panax notoginseng, 17.6 parts benzoin, and 20.6 parts pearl.

[0039] The preparation method of the above-mentioned traditional Chinese medicine composition is as follows:

[0040] (1) First, crush Panax notoginseng, pearl, ox horn tip, borneol, musk and bezoar into powder, pass them through a 100-mesh sieve and mix them to obtain a mixed powder;

[0041] (2) The red ginseng was then soaked in 8 times the volume of 60% ethanol aqueous solution. After percolation for 24 h, the solution was discharged at a rate of 2 ml / kg per minute. The percolation liquid was used to recover ethanol in a distillation pot until the concentration was below 10%. The solution was then concentrated to obtain red ginseng extract with a relative density of 1.25 g / mL at 60℃.

[0042] The attached piece is immersed in 6 times the volume fraction of 60% ethanol aqueous solution, and after 24 hours of percolation, the liquid is discharged at a rate of 2 ml / kg per minute. The ethanol in the percolate is recovered in a distillation kettle to a concentration of less than 10%, and concentrated to obtain an attached piece extract with a relative density of 1.25 g / mL at 60°C;

[0043] The safflower is immersed in 20 times the volume fraction of 60% ethanol aqueous solution, and after 24 hours of percolation, the liquid is discharged at a rate of 2 ml / kg per minute. The ethanol in the percolate is recovered in a distillation kettle to a concentration of less than 10%, and concentrated to obtain a safflower extract with a relative density of 1.25 g / mL at 60°C;

[0044] The toad venom is immersed in 11 times the volume fraction of 60% ethanol aqueous solution, and after 24 hours of percolation, the liquid is discharged at a rate of 2 ml / kg per minute. The ethanol in the percolate is recovered in a distillation kettle to a concentration of less than 10%, and concentrated to obtain an attached piece extract with a relative density of 1.25 g / mL at 60°C;

[0045] (3) Finally, the red ginseng extract, the attached piece extract, the safflower extract and the attached piece extract are combined, heated in a water bath, stirred uniformly, mixed with the mixed powder and the benzoin dissolved in 1.8 times the volume fraction of 75% ethanol aqueous solution, and mixed uniformly, and then dried to obtain the product.

[0046] Example 2

[0047] A traditional Chinese medicine composition, by weight fraction, consists of: red ginseng 80 parts, ox horn tip 35 parts, musk 5.5 parts, borneol 18 parts, safflower 5.5 parts, ox gall 36 parts, attached piece 36 parts, toad venom 14 parts, panax notoginseng 38 parts, benzoin 17 parts and pearl 21 parts.

[0048] The preparation method of the above traditional Chinese medicine composition is as follows:

[0049] (1) First, panax notoginseng, pearl, ox horn tip, borneol, musk and ox gall are crushed, mixed after passing through a 90-mesh sieve to obtain a mixed powder;

[0050] (2) Then, the red ginseng is immersed in 7 times the volume fraction of 50% ethanol aqueous solution, and after 24 hours of percolation, the liquid is discharged at a rate of 2 ml / kg per minute. The ethanol in the percolate is recovered in a distillation kettle to a concentration of less than 10%, and concentrated to obtain a red ginseng extract with a relative density of 1.35 g / mL at 60°C;

[0051] The attached piece is immersed in 5 times the volume fraction of 50% ethanol aqueous solution, and after 24 hours of percolation, the liquid is discharged at a rate of 2 ml / kg per minute. The ethanol in the percolate is recovered in a distillation kettle to a concentration of less than 10%, and concentrated to obtain an attached piece extract with a relative density of 1.35 g / mL at 60°C;

[0052] The safflower was immersed in 19 times volume fraction of 50% ethanol aqueous solution, and after percolation for 24 hours, the liquid was discharged at a speed of 2 ml / kg per minute. The ethanol in the percolation liquid was recovered in a distillation kettle until the concentration was below 10%, and then concentrated to obtain safflower extract with a relative density of 1.35 g / mL at 60°C;

[0053] The toad venom was immersed in 12 times volume fraction of 50% ethanol aqueous solution, and after percolation for 24 hours, the liquid was discharged at a speed of 2 ml / kg per minute. The ethanol in the percolation liquid was recovered in a distillation kettle until the concentration was below 10%, and then concentrated to obtain toad venom extract with a relative density of 1.35 g / mL at 60°C;

[0054] (3) Finally, the four extracts of red ginseng extract, toad venom extract, safflower extract and toad venom extract were combined, heated and fused in a water bath, stirred uniformly, mixed with the mixed powder and benzoin dissolved in 1.5 times volume fraction of 70% ethanol aqueous solution, mixed uniformly, and dried to obtain the product.

[0055] Example 3

[0056] A traditional Chinese medicine composition, by weight fraction, consists of: red ginseng 90 parts, ox horn tip 25 parts, musk 6.5 parts, borneol 17 parts, safflower 6.5 parts, ox gall 35 parts, toad piece 35 parts, toad venom 15 parts, panax notoginseng 39 parts, benzoin 18 parts and pearl 20 parts.

[0057] The preparation method of the above traditional Chinese medicine composition is as follows:

[0058] (1) First, panax notoginseng, pearl, ox horn tip, borneol, musk and ox gall were crushed, mixed after passing through a 110-mesh sieve to obtain a mixed powder;

[0059] (2) Then, the red ginseng was immersed in 9 times volume fraction of 70% ethanol aqueous solution, and after percolation for 24 hours, the liquid was discharged at a speed of 2 ml / kg per minute. The ethanol in the percolation liquid was recovered in a distillation kettle until the concentration was below 10%, and then concentrated to obtain red ginseng extract with a relative density of 1.35 g / mL at 60°C;

[0060] The toad piece was immersed in 7 times volume fraction of 70% ethanol aqueous solution, and after percolation for 24 hours, the liquid was discharged at a speed of 2 ml / kg per minute. The ethanol in the percolation liquid was recovered in a distillation kettle until the concentration was below 10%, and then concentrated to obtain toad piece extract with a relative density of 1.35 g / mL at 60°C;

[0061] The safflower was immersed in 21 times volume fraction of 70% ethanol aqueous solution, and after percolation for 24 hours, liquid was discharged at a speed of 2 ml / kg per minute. The percolation liquid was recovered in a distillation kettle to a concentration of less than 10%, concentrated, and the safflower extract with a relative density of 1.35 g / mL at 60°C was obtained.

[0062] The toad venom was immersed in 10 times volume fraction of 70% ethanol aqueous solution, and after percolation for 24 hours, liquid was discharged at a speed of 2 ml / kg per minute. The percolation liquid was recovered in a distillation kettle to a concentration of less than 10%, concentrated, and the extract of the toad venom tablet with a relative density of 1.35 g / mL at 60°C was obtained.

[0063] (3) Finally, the four extracts of red ginseng extract, toad venom extract, safflower extract and toad venom extract were combined, heated in a water bath, stirred uniformly, mixed with the mixed powder and benzoin dissolved in 2 times volume fraction of 80% ethanol aqueous solution, mixed uniformly, and dried to obtain the product.

[0064] Test Example 1

[0065] The inhibitory effects of the traditional Chinese medicine compositions prepared in Examples 1-3 on HepG2 tumor cells were studied respectively, and the experimental method was as follows:

[0066] Logarithmic growth period trypsin-digested HepG2 cells were resuspended in RPMI-1640 complete culture medium, blown and arranged into 1×10 5 / mL cell suspension, 100 μL was added to each well of a 96-well culture plate, and cultured in a 5% CO2, 37°C incubator for 24 hours. After the cells were completely adhered, normal control group, negative control group and experimental group were set up, with 8 replicate wells in each group.

[0067] Among them, the normal control group (containing cells 100 μL) was added with 100 μL RPMI-1640 complete culture medium; the negative control group only contained RPMI-1640 complete culture medium; the experimental group (containing cells 100 μL) was added with traditional Chinese medicine compositions prepared in Examples 1-3 with final concentrations of 20 μg / mL, 50 μg / mL and 100 μg / mL respectively, and cisplatin with a final concentration of 3 μg / mL. After incubation in the incubator for 48 hours, 10 μL CCK-8 solution was added, and incubation was continued for 4 hours. The supernatant was removed, and the OD value of each well was measured at 450 nm wavelength on a microplate reader. The inhibition rate of cells was calculated according to the following formula:

[0068] Inhibition rate = (negative control group OD value-experimental group OD value) ÷ (negative control group OD value-normal control group OD value) × 100%.

[0069] The data results are shown in Table 1. As shown in Table 1, when the concentration of the administered drug is 20-100 μg / mL, the traditional Chinese medicine composition of Examples 1-3 can inhibit the growth of liver cancer cells HepG2 to different degrees after administration. When the concentration of the administered drug is 50 μg / mL or 100 μg / mL, the inhibition rate of Examples 1-3 on liver cancer cells is significantly higher than that of the cisplatin group (p<0.05 or p<0.01), indicating that the anti-liver cancer cell activity of the traditional Chinese medicine composition of the examples is higher than that of the cisplatin group; when the concentration of the administered drug is 100 μg / mL, the inhibition effect on liver cancer cells is the best, and the inhibition rate of HepG2 cells is more than 97%.

[0070] Table 1 Inhibition effect of traditional Chinese medicine composition with different concentrations on HepG2 cells

[0071]

[0072] Note: Compared with the cisplatin group, * represents p<0.05, and ** represents p<0.01.

[0073] Test Example 2

[0074] The influence of the traditional Chinese medicine composition prepared in Research Example 1 on different liver cancer cell cycles was studied, and the detection method was as follows:

[0075] Logarithmic growth period HepG2 cells were taken, inoculated in a 6-well plate at a density of 1×10 5 6, and cultured in a 37°C, 5% CO2 incubator for 24 h. The cells were divided into a blank control group, an Example 1 group with administered drug concentrations of 100 μg / mL, 200 μg / mL and 300 μg / mL, respectively, and cultured for 48 h after different treatments. Then, the cells were collected into centrifuge tubes, resuspended by adding 1 mL pre-cooled PBS to each group, and uniformly blown. The centrifuge tubes were placed on a vortex shaker, and 9 mL of 70% frozen ethanol was added while shaking to fix, and placed at -20°C overnight. Before staining, the cells were washed with pre-cooled PBS, centrifuged at 4°C and 2000 rpm for 5 min, the fixing solution was removed, 500 μL of RNaseA was added for digestion at 37°C for 30 min, 25 μL of propidium iodide (PI) staining solution was added for mixing, and the cells were stained at room temperature for 30 min in the dark. The cells were filtered through a 200-mesh filter and collected in a flow cytometer sample tube. A 15 mW argon ion laser was used with an excitation wavelength of 488 nm and an emission wavelength of 530 nm. The percentage of AnnexinV-FITC and PI double-stained positive cells was calculated, and the percentage of each cycle stage of the cells was obtained. The test was repeated 3 times.

[0076] The detection results are shown in Table 2. As shown in Table 2, the traditional Chinese medicine composition prepared in Example 1 can effectively inhibit the normal conversion of the cell cycle, cause the HepG2 cells to accumulate in the G1 phase, and block the cells in the G1 phase, thereby preventing the mitosis of the cells and inhibiting the proliferation of the cells.

[0077] Table 2 Influence of the traditional Chinese medicine composition in Example 1 on the cell cycle of HepG2

[0078]

[0079] Test Example 3

[0080] The inhibitory effects of the traditional Chinese medicine compositions prepared in Examples 1-3 on the growth of HepG2 tumor-bearing nude mice were respectively studied, and the experimental method was as follows:

[0081] (1) Test sample: the traditional Chinese medicine compositions prepared in Examples 1-3.

[0082] (2) Experimental animal: BALB / c nude mice, 80, 5 weeks old, male, and weighing 19-22 g.

[0083] (3) Establishment of animal model: the HepG2 cells in logarithmic growth phase were taken after trypsin digestion, resuspended in RPMI-1640 complete culture medium, and blown and mixed into a 1×10 5 3 The tumor volume was increased to 100±10 mm

[0084] (4) Grouping and administration: the nude mice were randomly divided into 8 groups, 10 in each group, which were blank control group, model group, positive drug group, low-dose group, medium-dose group and high-dose group of Example 1, Example 2 group and Example 3 group, respectively.

[0085] Except for the blank control group, the animal models were established in the other groups. The positive drug group was orally administered with 30 mg / kg 5-fluorouracil; the low-dose group of Example 1 was orally administered with 20 mg / kg of the traditional Chinese medicine composition prepared in Example 1; the medium-dose group of Example 1 was orally administered with 50 mg / kg of the traditional Chinese medicine composition prepared in Example 1; the high-dose group of Example 1 was orally administered with 80 mg / kg of the traditional Chinese medicine composition prepared in Example 1; the Example 2 group was orally administered with 50 mg / kg of the traditional Chinese medicine composition prepared in Example 2; the Example 3 group was orally administered with 50 mg / kg of the traditional Chinese medicine composition prepared in Example 3; and the blank control group and the model group were given the same amount of normal saline. The oral administration was once a day, continuously for 30 days, and the nude mice were sacrificed on the 31st day, the tumor tissues were separated and weighed, the tumor inhibition rate was calculated, the tumor weight of the nude mice was the average value of the tumor weight of the nude mice in each group, and the calculation formula of the tumor inhibition rate was as follows: ​

[0086] Tumor inhibition rate (%) = (tumor weight of model group - tumor weight of administration group) ÷ tumor weight of model group × 100%.

[0087] (5) The tumor weight and tumor inhibition rate of each group of nude mice are shown in Table 3. It can be seen from Table 3 that the tumor inhibition rate of the medium dose group and the high dose group in Example 1, the Example 2 group and the Example 3 group is significantly higher than that of the positive drug group (p<0.05 or p<0.01), which indicates that the medium dose and the high dose of the traditional Chinese medicine composition in the present application can be applied to the treatment of liver cancer.

[0088] Table 3 Inhibition effect of traditional Chinese medicine composition of the present application on HepG2 tumor growth

[0089]

[0090] Note: Compared with the positive drug group, * represents p<0.05, and ** represents p<0.01.

[0091] Finally, it should be noted that the above content is only used to illustrate the technical solutions of the present application, and is not a limitation on the protection scope of the present application. Simple modifications or equivalent replacements of the technical solutions of the present application made by those skilled in the art do not deviate from the essence and scope of the technical solutions of the present application.

Claims

1. Use of a traditional Chinese medicine composition in the preparation of a drug for resisting liver cancer, characterized in that, The traditional Chinese medicine composition is composed of the following components in parts by weight: red ginseng 80-90 parts, cowhorn tip 25-35 parts, musk 5.5-6.5 parts, borneol 17-18 parts, safflower 5.5-6.5 parts, ox gall 35-36 parts, appendage piece 35-36 parts, toad venom 14-15 parts, panax notoginseng 38-39 parts, storax 17-18 parts, and pearl 20-21 parts.

2. Use according to claim 1, characterized in that, The preparation method of the traditional Chinese medicine composition comprises the following steps: (1) first, panax notoginseng, pearl, cowhorn tip, borneol, musk and ox gall are crushed, mixed after sieving, to obtain a mixed powder; (2) then, red ginseng, appendage piece, safflower and toad venom are respectively immersed in an ethanol aqueous solution, percolation is carried out, the percolate is collected, and concentration is carried out to obtain four kinds of extracts; (3) finally, the four kinds of extracts are combined, the mixed powder and storax dissolved in an ethanol aqueous solution are added, and mixing is carried out, drying is carried out, and the traditional Chinese medicine composition is obtained.

3. Use according to claim 2, characterized in that, In step (1), the mesh number of sieving is 90-110.

4. Use according to claim 2, characterized in that, In step (2), the immersion time is 24-36 h; the volume fraction of the ethanol aqueous solution is 50-70%; the relative density of the extract at 50-60℃ is 1.25-1.35 g / mL; red ginseng is immersed in 7-9 times the amount of ethanol aqueous solution; appendage piece is immersed in 5-7 times the amount of ethanol aqueous solution; safflower is immersed in 19-21 times the amount of ethanol aqueous solution; toad venom is immersed in 10-12 times the amount of ethanol aqueous solution.

5. Use according to claim 4, characterized in that, In step (3), the extracts after combination need to be heated in a water bath for fusion; the volume fraction of the ethanol aqueous solution is 70-80%.

6. Use according to claim 1, characterized in that, The medicine further comprises a pharmaceutically acceptable excipient, and the dosage form of the medicine is tablets, granules, capsules, pills or oral liquid.

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