Molecule identification method for Qingdao zoysia japonica and zoysia matrella filial generation authenticity

Abstract

The invention relates to a method for identifying authenticity of filial generation of zoysia and manilagrass, which belongs to the field of biological technology. The invention is specially for rapid and precise identification of authenticity of filial generation of Qingdao zoysia and manilagrass. Using a genome DNA template of nine zoysia filial generations and two parents Qingdao zoysia and manilagrass as the template, select three pairs of SRAP primers, namely primers capable of amplifying specific band not included in female parent on male parent. Perform amplifying and electrophoresis on the male parent, the female parent and the filial generation, and identify according as whether the F1 generation can amplify male parent specific band. The result shows that a filial generation is a real hybrid when each of the nine filial generations has male parent specific band. Thus, a molecular identification system of authenticity of filial generation of Qingdao zoysia and manilagrass is established, by which the authenticity of filial generation of Qingdao zoysia and manilagrass can be quickly identified, the method can also be used for hybrid variety fingerprint map construction andpurity identification.

Description

1. Technical Field [0001] The invention relates to a molecular identification method for the authenticity of the hybrid progeny of Zoysia plants, and belongs to the field of biotechnology. It is specially used for rapid and accurate detection of authenticity identification of the hybrid progeny of Qingdao Zoysiagrass and Zoysia matrella. 2. Background technology [0002] As a traditional breeding method, hybrid breeding has been quite mature, and it is a commonly used technical strategy in plant genetic improvement. Hybrid breeding using parents with obvious differences in turfgrass traits is an important way to select high-quality and excellent new lines. The flowering habit of Zoysia japonica is that the pistils ripen first, and the stamens ripen later. Research by Guo Hailin et al. has shown that the self-set rate of Zoysia japonica is 0.0-54%, with an average of 16.15%. Therefore, the hybrid offspring may be mixed with some self-bred species. . According to this, it is nece...

AI Technical Summary

Problems solved by technology

[0005] Technical problem The purpose of the present invention is to solve the field identification detection method and other molecular identification of the authenticity of Zoysia plant hybrid progeny in the prior art, which takes a long time, consumes a lot, and has poor accuracy. Molecular identification method for the authenticity of the hybrid offspring of Zoysia phyllophyllum and the identification of SRAP molecular markers on the hybrid offspring of Zoysia spp.

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