30 results about "Zoysia matrella" patented technology

The present cultivar TM9 (Zoysia matrella Merr.) advantageously has a growing rate of in terms of plant height less than half as compared with existing cultivars and requires mowing every 20 to 40 days during the summer so as to be maintained in fair condition and allows the amount of fertilizer to be reduced to almost less than half.

The invention discloses a method for inducing differentiation seedling of manilagrass callus. The method comprises the following steps of: selecting embryonal callus of manilagrass, arranging embryonal callus of manilagrass in a solid medium in which the content of 6-Benzylaminopurine being 0-0.4mg / L and the content of trans-zeatin being 0-0.4mg / L, and growing into seedlings after four weeks. According to the method for inducing the differentiation seedling of the manilagrass callus, disclosed by the invention, a culture medium containing two hormones, namely 6-Benzylaminopurine and trans-zeatin, callus subculturing for a long term is regenerated and cultivated, the regeneration rate of the manilagrass callus subculturing for a long term is remarkably improved, a problem that the regeneration rate of the manilagrass callus subculturing for many years is low is effectively solved, the tissue culture regeneration system of the manilagrass is further modified, and foundation is established for a cell engineering breeding and gene transformation research; and the method is simple to operate and has high efficiency.

The invention discloses a method for rapidly propagating Zoysia matrella to form lawns in a large scale, belonging to the technical field of cultivating lawns. The method provided by the invention comprises the following steps of: (1) ploughing soil and pouring water to wet the soil; after weeds are germinated, spraying herbicide and tightly pressing; paving a paddy layer with the thickness of 1-3 cm on the soil layer and then paving yellow sand layer with the thickness of 2-4 cm and mud with the thickness of 2-4 cm in sequence to form a lawn bed; (2) chopping creeping stems of the Zoysia matrella lawns and spraying rooting agent; uniformly sowing the stems into a lawn bed; lightly crushing grass stems and pouring water to accelerate rooting; (3) alternatively spraying 30-60 ml of glyphosate with the concentration of 20% and 300-500 ml of paraquat with the concentration of 10% into each acre every 10-20 days to remove weeds; spraying water for 2-3 times every day and applying additional fertilizer into the lawns to accelerate growth; after three months, producing the lawns. The method provided by the invention is simple and practical, combines the characteristic that the Zoysia matrella grows fast and utilizes traditional multiple technologies for propagating and cultivating lawns to optimize; the method can cultivate and produce lawns in a large scale, and has a fast lawn producing speed and a high lawn quality.

The invention belongs to the field of molecular biology, and discloses a salt-tolerant gene ZmPDI in a halophyte zoysia matrella as well as a plant expression vector and application thereof. The sequence of the salt-tolerant gene ZmPDI in the zoysia matrella is SEQ ID NO. 1. The plant expression vector is obtained by carrying out double enzyme digestion on ZmPDI through BamHI and EcoRV, then inserting the ZmPDI into a gateway entry vector pENTR1A, and finally subjecting the ZmPDI and a pEarleyGate103 expression vector plasmid to recombination reaction. The ZmPDI in the zoysia matrella, which is provided by the invention, is a novel salt-tolerant gene; the gene can be used for improving the salt tolerance of a plant, and can be applied to the creation of novel salt-tolerant germplasm and the improvement of a plant variety.

The invention relates to a method for identifying authenticity of filial generation of zoysia and manilagrass, which belongs to the field of biological technology. The invention is specially for rapid and precise identification of authenticity of filial generation of Qingdao zoysia and manilagrass. Using a genome DNA template of nine zoysia filial generations and two parents Qingdao zoysia and manilagrass as the template, select three pairs of SRAP primers, namely primers capable of amplifying specific band not included in female parent on male parent. Perform amplifying and electrophoresis on the male parent, the female parent and the filial generation, and identify according as whether the F1 generation can amplify male parent specific band. The result shows that a filial generation is a real hybrid when each of the nine filial generations has male parent specific band. Thus, a molecular identification system of authenticity of filial generation of Qingdao zoysia and manilagrass is established, by which the authenticity of filial generation of Qingdao zoysia and manilagrass can be quickly identified, the method can also be used for hybrid variety fingerprint map construction andpurity identification.

The invention belongs to the field of molecular biology and discloses a salt-resistant gene ZmGnTL of a halophyte zoysia matrella and an expression vector and an application thereof. The sequence of a novel salt-resistant gene ZmGnTl in zoysia matrella is SEQ ID NO.1. The plant expression vector is obtained by performing a recombination reaction with pEarleyGate103 expression vector plasmids after inserting ZmGnTL after double enzyme digestion of BamHI and NotI into a gateway entry vector pENTR1A. The ZmGnTL of zoysia matrella provided by the invention is the novel salt-resistant gene which can improve the salt resistance of the plant and can be applied to creating salt-resistant novel germplasms and improving plant varieties.

The invention relates to a method for ecological remediation of sea island bare land. The method includes steps: (1) shallow excavating of the sea island bare land; (2) replacement soil backfilling; (3) landfill of controlled-release active nutritional packages; (4) sowing of zoysia matrella; (5) cuttage of procumbent juniper. By backfilling of improved mellow soil, a soil base of the sea island bare land can be improved, air permeability of the mellow soil is greatly improved due to addition of fiber materials, and less proneness to caking in long-time use is realized after the mellow soil is backfilled to sea island land; due to organic fertilizers, water retaining agents and soil stabilizing agents, the backfilled mellow soil is rich in nutrition, great in water retaining performance and high in soil property stability and provides sufficient nutrients and moisture for early growth of zoysia matrella and procumbent juniper. The controlled-release active nutritional packages are rich in natural organics and adsorbed and stored trace elements and buried into the improved mellow soil to continuously and slowly release and degrade under rain flushing and natural diffusion, so that nutrient loss can be greatly avoided, and continuous nutrient supply can be provided for later growth of zoysia matrella and procumbent juniper.

The invention belongs to the field of molecular biology and discloses a salt-resistant gene ZmDUF1644 of a halophyte zoysia matrella, and an expression vector and an application thereof. The sequence of a novel salt-resistant gene ZmDUF1644 in zoysia matrella is as shown in SEQ ID NO.1. The plant expression vector is obtained by performing a recombination reaction with pEarleyGate103 expression vector plasmids after inserting ZmDUF1644 subjected to double enzyme digestion of BamHI and NotI into a gateway entry vector pENTR1A. The ZmDUF1644 of zoysia matrella provided by the invention is the novel salt-resistant gene which can improve the salt resistance of the plant and can be applied to creating salt-resistant novel germplasms and improving plant varieties.

The present culivar ‘TMX’ (Zoysia matrella Merr.) advantageously has a very slow growing rate in terms of plant height as compared with existing cultivars and requires mowing not more than once a year so as to be maintained in fair condition and allows the amount of fertilizer to be reduced. It also has a high-level of shade tolerance.