30 results about "Zoysia matrella" patented technology

The invention relates to a method for identifying authenticity of filial generation of zoysia and manilagrass, which belongs to the field of biological technology. The invention is specially for rapid and precise identification of authenticity of filial generation of Qingdao zoysia and manilagrass. Using a genome DNA template of nine zoysia filial generations and two parents Qingdao zoysia and manilagrass as the template, select three pairs of SRAP primers, namely primers capable of amplifying specific band not included in female parent on male parent. Perform amplifying and electrophoresis on the male parent, the female parent and the filial generation, and identify according as whether the F1 generation can amplify male parent specific band. The result shows that a filial generation is a real hybrid when each of the nine filial generations has male parent specific band. Thus, a molecular identification system of authenticity of filial generation of Qingdao zoysia and manilagrass is established, by which the authenticity of filial generation of Qingdao zoysia and manilagrass can be quickly identified, the method can also be used for hybrid variety fingerprint map construction and purity identification.

A feed for promoting growth of calves and a preparation method thereof are disclosed. The feed comprises the following raw materials, by weight, 300-400 parts of soyabean protein powder, 200-300 parts of corn bran, 200-300 parts of barley flour, 6-8 parts of soy sauce, 3-4 parts of mature vinegar, 2-3 parts of star aniseed powder, 2-3 parts of chilli sauce, 30-40 parts of pork jelly, 20-30 parts of fish gel, 70-80 parts of coagulated pig blood, 40-50 parts of coagulated duck blood, 20-30 parts of green pepper, 10-20 parts of onion, 10-15 parts of cottonseed oil, 50-60 parts of lagenaria siceria powder, 20-40 parts of mangosteen powder, 3-4 parts of Ligusticum wallichii, 2-3 parts of white peony root, 2-3 parts of Poria cocos, 1-2 parts of cortex lycii, 8-15 parts of coix seed oil, 5-10 parts of aloe freeze-dried powder, 3-5 parts of white carbon black, 50-60 parts of zoysia japonica, 40-50 parts of zoysia matrella, 20-30 parts of Cucurbita pepo L, 10-20 parts of spearmint, 5-10 parts of cypress leaf, 2-4 parts of gynostemma pentaphylla powder, 30-60 parts of rice bran, 20-30 parts of a phagostimulant and a proper amount of water.

The invention discloses a young turtle fattening feed and a manufacturing method thereof. The young turtle fattening feed is prepared from hoof and horn meal, wool powder, dried locust powder, shrimp meal, coarse corn grains, sorghum, bone meal, green hyacinth beans, grapefruits, corn syrup, whole egg powder, zoysia matrella, nullipore, zoysia japonica, radix ophiopogonis, grist, semen coicis, ginkgo seeds, carrots, bengal waterdropwort herb, california burclover, white pepper, salt, agar, cotton stalks, edible bamboo charcoal powder and the like. The feed solves the problems that a traditional young turtle feed lacks nutrients and is poor in palatability, the manufactured feed is coarse, the young turtle is low in appetite, the weight of the young turtle is increased slowly, and the fattening effect is poor, the obtained feed is rich in nutrient, fine and smooth in taste, fragrant in taste, the appetite of the young turtle can be enhanced, weight increase and meat production are promoted, and the young turtle fed by the feed is high in appetite, rapidly gains weight, is good in fattening effect, can greatly improve economic benefits of young turtle culture and has the quite high popularization value.

The invention discloses a method for genetic transformation of zoysia matrella mediated by agrobacterium tumefaciens. The method includes the steps of the concentration of a bacterial solution, the infection time, the co-culture time, the antibiotic concentration, acclimatization and transplantation, PCR detection of transgenic plants and the like. The method includes the following steps that an optimal genetic transformation system is that an OD600 value of the bacterial solution is 0.4, infection is conducted for 30 min, and selective culture is conducted after co-culture is conducted for 3d; the optimal bacteriostatic concentration of Timentin in a selective culture phase is 250 mg/L, the optimum selection pressure for hygromycin callus screening is 40 mg/L, and the optimum concentration of root taking screening is 15 mg/L; and histochemical analysis and PCR identification of GUS activity show that a target gene is successfully transferred into a genome of the zoysia matrella. Theabove research provides important technical support for gene function research and molecular breeding of the zoysia matrella. The method has the advantages that a stable and high-efficiency zoysia matrella genetic transformation system is obtained, the convenience is provided for genetic improvement of the zoysia matrella, and meanwhile, a key preliminary foundation is laid for verification of functional genes of the zoysia matrella.

The invention discloses a method for inducing differentiation seedling of manilagrass callus. The method comprises the following steps of: selecting embryonal callus of manilagrass, arranging embryonal callus of manilagrass in a solid medium in which the content of 6-Benzylaminopurine being 0-0.4mg/L and the content of trans-zeatin being 0-0.4mg/L, and growing into seedlings after four weeks. According to the method for inducing the differentiation seedling of the manilagrass callus, disclosed by the invention, a culture medium containing two hormones, namely 6-Benzylaminopurine and trans-zeatin, callus subculturing for a long term is regenerated and cultivated, the regeneration rate of the manilagrass callus subculturing for a long term is remarkably improved, a problem that the regeneration rate of the manilagrass callus subculturing for many years is low is effectively solved, the tissue culture regeneration system of the manilagrass is further modified, and foundation is established for a cell engineering breeding and gene transformation research; and the method is simple to operate and has high efficiency.

The present invention provides a planting method for zoysia matrella in green belts on both sides of a carriageway, and relates to the technical field of urban greening plant cultivation. The method is characterized by comprising the following steps of soil treatment, plant immersion, plant fertilization and plant arrangement. The method disclosed by the present invention is reasonable. With the method, zoysia matrella is planted conveniently; sufficient nutrients are ensured; and activity and freezing resistance of plants can be improved.

The invention relates to a method for ecological remediation of sea island bare land. The method includes steps: (1) shallow excavating of the sea island bare land; (2) replacement soil backfilling; (3) landfill of controlled-release active nutritional packages; (4) sowing of zoysia matrella; (5) cuttage of procumbent juniper. By backfilling of improved mellow soil, a soil base of the sea island bare land can be improved, air permeability of the mellow soil is greatly improved due to addition of fiber materials, and less proneness to caking in long-time use is realized after the mellow soil is backfilled to sea island land; due to organic fertilizers, water retaining agents and soil stabilizing agents, the backfilled mellow soil is rich in nutrition, great in water retaining performance and high in soil property stability and provides sufficient nutrients and moisture for early growth of zoysia matrella and procumbent juniper. The controlled-release active nutritional packages are rich in natural organics and adsorbed and stored trace elements and buried into the improved mellow soil to continuously and slowly release and degrade under rain flushing and natural diffusion, so that nutrient loss can be greatly avoided, and continuous nutrient supply can be provided for later growth of zoysia matrella and procumbent juniper.

The invention belongs to the field of molecular biology and discloses a salt-resistant gene ZmGnTL of a halophyte zoysia matrella and an expression vector and an application thereof. The sequence of a novel salt-resistant gene ZmGnTl in zoysia matrella is SEQ ID NO.1. The plant expression vector is obtained by performing a recombination reaction with pEarleyGate103 expression vector plasmids after inserting ZmGnTL after double enzyme digestion of BamHI and NotI into a gateway entry vector pENTR1A. The ZmGnTL of zoysia matrella provided by the invention is the novel salt-resistant gene which can improve the salt resistance of the plant and can be applied to creating salt-resistant novel germplasms and improving plant varieties.

The invention discloses an urban courtyard afforestation configuration mode for reducing PM 2.5, which comprises the following steps: in the first ten-day period of March, according to the plant configuration standard, digging plant pits and applying cake fertilizer or compound fertilizer in the plant pits; at the front courtyard, adopting the configuration mode of planting zelkova schneiderianas, southern magnolias, sweet-scented osmanthus trees, crape myrtle trees, red maples, pomegranate trees, rhododendron trees, loropetalum chinense var. rubrums and ground cover plants (gardenia and radix ophiopogonis); at the rear courtyard, adopting the configuration mode of planting hackberries, sweet-scented osmanthus trees, Chinese redbud, euonvmus japonicus cv.aureo-ma, camellia and ground cover plants (zephyranthes candida); on the east and west sides of the courtyard, adopting the three-dimensional configuration mode of planting koelreuteria paniculata, banana shrub, loropetalum chinense var. rubrums, pittosporum tobira and ground cover plants (gardenia and zoysia matrella). According to the invention, based on the spatial distribution pattern of urban PM 2.5, the afforestation position effect and the arbor and shrub multi-layer configuration effect of urban communities are strengthened, and the PM 2.5 exhausted by motor vehicles and heavy metal pollutants in the communities are reduced to the greatest degree. With adoption of the urban courtyard afforestation configuration mode disclosed by the invention, the concentration of the PM 2.5 can be decreased for 1.86 times as compared with that of adopting the conventional afforestation mode.

The invention relates to the technical field of planting plate water planting, and discloses a water tank planting plate cultivation system forzoysia matrella, which comprises a plurality of mutually spliced cultivation units, each cultivation unit comprises an inner shell and an outer shell detachably connected with the outer shell, the inner shell is connected with the outer shell through a plurality of adjusting bolts, the outer shell is connected with the inner shell through a plurality of adjusting bolts, a planting groove used for planting the zoysia matrella is formed in the middle of each inner shell, a plurality of through holes are formed in the side wall of each inner shell, a plurality of protruding blocks are evenly distributed on the outer side of each inner shell, a penetrating hole is formed in each protruding block, each outer shell is movably arranged on the outer side of the corresponding inner shell in a sleeving mode, an annular groove is formed in the top surface of each inner shell along the inner ring, a plurality of spiral connecting bases corresponding to the protruding blocks are distributed at the bottom of each groove, and threaded grooves are formed in the top surfaces of the spiral connecting bases. The water tank planting plate cultivation system is convenient to use for water planting, can completely discharge cultivation soil after the zoysia matrella is mature, and is convenient to use for lawn planting in the later period.

The invention belongs to the field of molecular biology and discloses a salt-resistant gene ZmDUF1644 of a halophyte zoysia matrella, and an expression vector and an application thereof. The sequence of a novel salt-resistant gene ZmDUF1644 in zoysia matrella is as shown in SEQ ID NO.1. The plant expression vector is obtained by performing a recombination reaction with pEarleyGate103 expression vector plasmids after inserting ZmDUF1644 subjected to double enzyme digestion of BamHI and NotI into a gateway entry vector pENTR1A. The ZmDUF1644 of zoysia matrella provided by the invention is the novel salt-resistant gene which can improve the salt resistance of the plant and can be applied to creating salt-resistant novel germplasms and improving plant varieties.

The invention discloses a method for planting zoysia matrella on green belts at two sides of a traffic lane, relating to the technical field of urban green plant planting. The method is characterized by comprising the following steps: soil treatment, plant soaking, plant fertilization and plant clearing. The method is reasonable, the planting is convenient, rich nutrients are ensured, and the activity and freezing resistance of plants can be improved.

The invention discloses an interspecific hybridization breeding method for a new zoysia plant variety and belongs to the technical field of artificial interspecific hybridization breeding of zoysia plants. The method comprises the following steps: with Zoysia japonica and Z.sinica as female parents, and Z.matrella and Z.tenuifolia as male parents, performing artificial hybridization, thereby obtaining the new variety of zoysia plant interspecific hybridization with excellent characters of the parents. The flowering habit of the zoysia plants is fully utilized, namely, when stamens just appear, the anther is not loose, and the stamens are easily removed before the stamens just appear and the anther is loose, so that the purity of filial generations is guaranteed, and the breeding efficiency is improved. The established hybridization breeding system plays an important role in culturing the new zoysia plant variety and promoting the development of lawns in China.

The present culivar TMX (Zoysia matrella Merr.) advantageously has a very slow growing rate in terms of plant height as compared with existing cultivars and requires mowing not more than once a year so as to be maintained in fair condition and allows the amount of fertilizer to be reduced. It also has a high-level of shade tolerance.