Method for inducing differentiation seedling of manilagrass callus

A technology of Zoysia rugosa and callus, which is applied in the field of inducing Zoysia rugosa callus to differentiate into seedlings, can solve the problems of low regeneration rate of Zoysia rugosa callus, and achieve the goal of solving callus The effect of low tissue regeneration rate, perfect tissue culture regeneration system, and simple operation

Inactive Publication Date: 2012-08-15
ZHEJIANG UNIV
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  • Claims
  • Application Information

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Problems solved by technology

[0006] The invention provides a method for inducing callus differentiation into seedlings of Zoysia rugosa, the method is simpl

Method used

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  • Method for inducing differentiation seedling of manilagrass callus
  • Method for inducing differentiation seedling of manilagrass callus
  • Method for inducing differentiation seedling of manilagrass callus

Examples

Experimental program
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Embodiment

[0022] (1) On the ultra-clean workbench, select a hard, full and yellow-green diameter embryogenic callus (such as figure 2 shown);

[0023] (2) Transfer the above-mentioned embryogenic callus to the solid medium containing 6-BA and ZR, and inoculate 20 grains in each cylindrical flask;

[0024] Solid medium formula: 1.1g / L MS powder, 30g / L sucrose, 3g / L Phytagel plant gel, pH 5.8-6.0, and the ratio of 6-BA and ZR is as described in Table 1;

[0025] (3) Place the inoculated cylindrical flask in a constant-temperature sterile culture room at 25±2°C, and cultivate under 12h light / day;

[0026] (4) Observe and compare its regeneration every day, and differentiate into seedlings (such as image 3 Shown), the average regeneration rate of callus was counted, and the results are shown in Table 1.

[0027] The callus regeneration rate was calculated according to the following formula:

[0028] Regeneration rate (%) = number of callus pieces of regenerated seedlings / total number ...

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Abstract

The invention discloses a method for inducing differentiation seedling of manilagrass callus. The method comprises the following steps of: selecting embryonal callus of manilagrass, arranging embryonal callus of manilagrass in a solid medium in which the content of 6-Benzylaminopurine being 0-0.4mg/L and the content of trans-zeatin being 0-0.4mg/L, and growing into seedlings after four weeks. According to the method for inducing the differentiation seedling of the manilagrass callus, disclosed by the invention, a culture medium containing two hormones, namely 6-Benzylaminopurine and trans-zeatin, callus subculturing for a long term is regenerated and cultivated, the regeneration rate of the manilagrass callus subculturing for a long term is remarkably improved, a problem that the regeneration rate of the manilagrass callus subculturing for many years is low is effectively solved, the tissue culture regeneration system of the manilagrass is further modified, and foundation is established for a cell engineering breeding and gene transformation research; and the method is simple to operate and has high efficiency.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation, and in particular relates to a method for inducing callus differentiation of Zoysia sulcifolia into seedlings. Background technique [0002] Plant tissue culture technology has been widely used in various research fields, penetrated into breeding, genetics, molecular biology and other disciplines, and has become an important research technology and means in life sciences. In a narrow sense, tissue culture refers to the cultivation of various parts of plants, such as cambium, parenchyma, mesophyll, endosperm, etc. Regenerated plants are formed after redifferentiation. [0003] Zoysia matrella (Gramineae Zoysia matrella (L.) Merr.) is a perennial herb of the genus Zoysia in the Poaceae Teffae subfamily, also known as Manila grass, widely distributed in tropical and subtropical regions of Asia and Australia, my country There are wild species in Fujian, Guangdong, Guangxi and other places,...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 顾敏霞柴明良张丽邵丽达陈曙贾玉芳
Owner ZHEJIANG UNIV
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