102 results about "Zoysia japonica" patented technology

The invention provides a method of mixed planting of cool-season turf grass and warm-season turf grass, which includes: sowing seeds of Zoysia japonica and seeds of tall fescue according to a proportion of 1:1; sowing the seeds of Zoysia japonica prior to the seeds of tall fescue; and setting seeding quantity of each type of the seeds to be 15g/m2 at the temperature of 30 DEG C to 35 DEG C. By the method, the problem that the warm-season turf grass is withered and yellow in winter, short in green period, difficult to overwinter and the like is solved, the problem that the cool-season turf grass tends to wither in summer, tends to hibernate, is susceptible to diseases and the like is also solved, quality of turf in transition areas is improved, and management cost is lowered.

The present invention relates to a low conservation zoysia japonica lawn planting method. Said invention is characterized by that it selects the local lawn plant material-zoysia japonica which can be adapted to low conservation and utilizes seed stem cultivation method or turf directly-laying method to plant lawn, before said lawn is planted, the weeds must be removed and the fertilizer can be applied in the place where the zoysia japonica lawn can be planted.

The invention relates to a microsclerotium bacterial strain and a method for biological weed control therewith, belonging to the application of microorganism for agricultural plant protection. The microsclerotium bacterial strain is (SC64) CGMCC with number thereof being NO.2934. The use dosage of weeding bacterial particles obtained by solid fermentation of the bacterial strain ranges from 30g.m-2-150 g.m-2, and the weeding bacterial particles can be applied to poaceae crops such as corn, rice, wheat, barley, sugar cane, kaoliang, millet and poaceae plants such as lawn bermuda grass, zoysia japonica, festuca arundinacea, poa annua, agrostis stolonifera, axonopus compressus, eremochloa ophiuroides, ditch millet to control growth of broad-leaf weed. In addition, in particular, the weeding bacterial particles can be applied to control destructive invasive foreign weeds such as eupatorium adenophorum, solidago canadensis, horseweed herb, conyza sumatrensis, flaveria bidentis, mikania micrantha, annual fleabane herb and fragrant eupatorium herb. 80% of the target weeds can be brought under control, thus having the characteristics of safety and environmental friendliness.

The invention provides a method for updating a deteriorated grassland bluegrass lawn at low cost in the north. Weed control, pruning height control, lawn partition area and turf block size determination, rooting hastening with grass blocks soaked in pesticide, determination of the number of turf blocks planted in a community per square meter and planting positions, compacting, watering, sand covering, growth regulator application and other technologies are applied, on the basis of the natural rules of plant interspecific competition and the survival of the fittest, zoysia japonica can easily invade the deteriorated grassland bluegrass lawn due to strong creeping stems and the strong growing vigor and competitiveness, other grass seeds can hardly invade the lawn once the population of the zoysia japonica is formed, and therefore the aim of updating the deteriorated grassland bluegrass lawn at low cost is achieved. The effective method can be applied to the production practice of landscaping in a large area, can solve the problem of a deteriorated cool-season lawn, and can lower updating cost.

The invention discloses an organic fertilizer and a preparation method thereof. The organic fertilizer comprises the following raw materials by weight: 12 to 16 parts of blue algae, 6 to 8 parts of diatomite, 20 to 22 parts of dunaliella, 15 to 17 parts of spinaciaoleracea, 12 to 14 parts of eucheuma, 6 to 8 parts of rice washing water, 14 to 16 parts of wormcast, 8 to 10 parts of apple snail, 25 to 27 parts of poultry dung, 12 to 16 parts of cottonseed meal, 7 to 9 parts of zoysia japonica, 12 to 16 parts of sudan grass, 13 to 15 parts of raphanussativus L., 18 to 20 parts of swill, 4 to 6 parts of an EM stock solution, and 18 to 20 parts of rauhkalk. The organic fertilizer is sufficient in fertility and high in efficiency.

The invention discloses a method for accelerating plant community succession, promoting wetland ecological system functions and quickly restoring ecological functions of coastal wetlands. The technical scheme is as follows: the method is characterized by comprising the steps as follows: 1) preparation of bio-fertilizer: seeds of suaeda of pioneer species of the coastal wetlands are sowed in large experimental fields, suaeda plants are dug, root soil is collected and mixed uniformly with crushed plant fragments, the mixture is bagged for water logging; 2) fertilization and soil ploughing: in spring, the bio-fertilizer is spread on the degraded soil of the coastal wetlands, and the soil is ploughed; 3) colonization of the sowed suaeda and soil transformation: the seeds of the suaeda are sowed after the soil is ploughed, and colonization lasts for one year; 4) continued transformation and upgrading of the soil: in the following spring, seeds of plants such as zoysia japonica, apocynum venetum and the like are sowed, so that transient plant communities of the wetlands grow quickly; and 5) natural restoration of the ecological functions of the wetlands.

The invention discloses a method of improving the asexual breeding speed of creepers of zoysia japonica plants. The method of improving the asexual breeding speed of creepers of zoysia japonica plants includes the following steps: 1) selecting plants and processing the plants; 2) sowing: sowing the processed creepers on a turf-bed; 3) covering soil (sand) and pressing: covering the creepers with fine soil (sand), pressing for one time to enable grass blades to closely contact the sand soil; and 4) covering and watering for moisture retention, pouring water over the soil at the first time and watering 1-2 times every day to enable the soil to be maintained in a moisture state for 6-7d until adventitious roots come up while the covering is taken away, and then watering every one week, so that a bed can form after 2-3 weeks in the growth season. Compared with the prior art, the method of improving the asexual breeding speed of creepers of zoysia japonica plants can significantly improve the asexual breeding speed of creepers of zoysia japonica and is conductive to popularization and application creepers of zoysia japonica plants, by means of processing before sowing the creepers of zoysia japonica and the combination with other processing methods.

The invention discloses bipolaris panici-miliacei (Yi.Nisik.) Shoemaker (SX-5-2) and a screening method and application thereof. The bipolaris panici-miliacei (Yi.Nisik.) Shoemaker (SX-5-2) has been preserved in the general microbiological center of Chinese microbial species preservation management committee, and the preservation number is CGMCC No.13887. The concentration of conidium which utilizes the bipolaris panici-miliacei (Yi.Nisik.) Shoemaker (SX-5-2) is more than 104 per milliliter, and the bipolaris panici-miliacei (Yi.Nisik.) Shoemaker (SX-5-2) is used for biological weeding, can be safely used in Oryza sativa L., Triticum aestivum L., sorghum bicolor (L.) Moench, Glycine max (Linn.) Merr., Gossypium spp and vegetable crop fields as well as Festuca elata Keng ex E. Alexeev and Zoysia japonica Sreud., and is used for controlling gramineous weeds such as Digitaria sanguinalis (L.) Scop, Eleusine indica (L.) Gaertn, Setaria viridis (L.) Beauv., Echinoochloa crusgalli (l.) Beauv., Leptochloa chinensis (L.) Nees, Microstegium vimineum (Trin.) A. Camus, Alopecurus aequalis Sobol. and Poa annua L., as well as microstegium related genera plant such as Eccoilopus cotulifer (Thunb.) A., Miscanthus sinensis anderss., Eulalia speciosa, Microstegium japonicum, Arthraxon hispidus (Thunb.) makino, Sorghym sudanense (Piper stapf). The controlling effect on target weeds can reach more than 80%. The bipolaris panici-miliacei (Yi.Nisik.) Shoemaker (SX-5-2) has the characteristics of safety and environmental protection.

The invention relates to a method for identifying authenticity of filial generation of zoysia and manilagrass, which belongs to the field of biological technology. The invention is specially for rapid and precise identification of authenticity of filial generation of Qingdao zoysia and manilagrass. Using a genome DNA template of nine zoysia filial generations and two parents Qingdao zoysia and manilagrass as the template, select three pairs of SRAP primers, namely primers capable of amplifying specific band not included in female parent on male parent. Perform amplifying and electrophoresis on the male parent, the female parent and the filial generation, and identify according as whether the F1 generation can amplify male parent specific band. The result shows that a filial generation is a real hybrid when each of the nine filial generations has male parent specific band. Thus, a molecular identification system of authenticity of filial generation of Qingdao zoysia and manilagrass is established, by which the authenticity of filial generation of Qingdao zoysia and manilagrass can be quickly identified, the method can also be used for hybrid variety fingerprint map construction and purity identification.

The invention provides a method for regenerating Japanese lawngrass. It comprises pretreating explant, inoculating callus inducing culture medium and getting callus; selecting embryogenic callus and inoculating it in subculture medium for subculture, or inoculating regenerative culture medium for regenerative plant differentiation; the callus inducing culture medium contains 2, 4- D 3- 6mg/l, 6- BA or KT 0.05- 0.2 kg/l and CuSO4 2- 3.5 mg/l. The inducing rate for explant callus can reach 90%, embryogenic callus inducing rate can reach 30%, and the regenerating rate for callus can reach 45%. The regenerative plant is characterized by strong tillering and rooting ability, high survival rate of plant after strong seedling and hardening seedling, which reaches above 87.5%. The replanted plant can propagate in field normally.

The invention relates to a method for promoting zoysia japonica seed germination. The method comprises the following steps of 1, selecting zoysia japonica seeds, and immersing the zoysia japonica seeds in sodium hydroxide, and 2, carrying out immersion disinfection on the immersed zoysia japonica seeds by disinfection reagents such as ethanol, a sodium hypochlorite solution and Tween. The method improves a zoysia japonica germination rate, shortens germination time, has simple processes, adopts a reasonable germination rate evaluation method, provides an effective basis for scientific research and production and promotes commercial development and popularization.

The invention provides a zoysia japonica seedling overwintering protection method for delayed sowing in the northern area. By the application of the technologies of water and fertilizer processing, mulching film temperature increasing for prolonging of the growing period, rolling in the period of dormancy, soil covering for protection, scientific soil covering ratio, rolling and soil supplement during thawing in the early spring, watering and scarification in the period of seedling establishment, and the like, the growing period of a primary lawn sowed in a delayed mode is prolonged, all growth indexes reach the standard required before a normal lawn overwinters after seedlings grow for another 20-25 days serving as the prolonged growing period, and the seedlings can overwinter successfully due to protection of overwintering protection measures. The zoysia japonica seedling overwintering protection method solves the problem that due to delaying of the construction progress, the time of completion of the whole landscape engineering is affected, and also achieves the technical aim that warm-season lawns overwinter successfully.

The invention discloses and provides a method for creating a high-frequency regeneration system of zoysia japonica. The method comprises the followings steps that a single zoysia japonica seed is sequentially subjected to induction culture, first generation subculture, second generation subculture and third generation subculture to obtain the high-frequency regeneration system of the zoysia japonica. The experiments prove that the invention provides the method for creating a high-frequency regeneration callus system or a regeneration plant and a special culture medium. According to the invention, Japanese zoysia japonica varieties are screened, a single seed is taken as an explant, different callus systems are respectively induced, then the callus system with high differentiative capacity is screened after proportioning combination of the culture medium and desiccation subcultures for multiple times, and a large quantity of high-frequency regeneration calluses propagated in each system are homogeneous. Finally, the method for creating the high-frequency regeneration system of zoysia japonica is built to provide a practical method to improve the genetic transformation efficiency and the tissue culture regeneration rate of zoysia japonica.

The invention relates to a method for improving seed germination rate of Zoysia japonica, for solving the problem of low seed germination rate of Zoysia japonica. The method comprises the steps of soaking seeds of Zoysia japonica in 3-5% NaOH solution under regular stirring for 4-5 h, filtering to collect seeds, placing in a container, rinsing with flowing water until effluent water pH is neutral, taking out the seeds, adding into a spin-drying, spin-drying the seeds in the spin-drying barrel for 8-10 min, soaking in 0.08-0.1% KNO3 solution for 20-40 min, taking out the seeds, adding into the spin-drying, spin-drying in the spin-drying barrel for 20-25 min, airing outdoors until water content reaches 10-15%, and bagging. The invention significantly improves seed germination rate of Zoysia japonica, reaches and even exceeds sowing lawn construction technical requirement of Zoysia japonica.

The invention discloses a method for increasing cycle conversion utilization of Zoysia japonica high frequency regeneration series. The method uses the Zoysia japonica high frequency regeneration callus series for performing gene transformation, the Zoysia japonica high frequency regeneration callus series can be used to obtain the regeneration plant stem for performing gene transformation, a regeneration method for the Zoysia japonica regeneration plant stem and a culture medium. The method provided in the invention provides a high efficiency approach for the cycle utilization and gene transformation of the Zoysia japonica high frequency regeneration series, and has wide application prospect on the research and production field of the Zoysia japonica.

The invention clones GA20 oxydase gene from an excellent grass seed for lawn-Korea lawn grass and constructs the plant expression vector for the grass seed. By degenerating PCR and RACE method, GA20 oxydase gene is cloned from wild Korea lawn grass of China. The total length of the gene is 1311 bp, the code area length is 1109 bp, the code area is 96-1205 bp including 369 amino acid. The gene is of 65% homology with wheat and rice. The login no. on GenBank is DQ645453. In addition, the invention constructs the positive and reverse expression vector for the GA20 oxydase gene based on pC1303. The vector contains GUS report gene and hygromix marking gene, can be used for seed breeding in gene engineering of lawn grass so as to culture short transgenosis lawn grass.

A feed for promoting growth of calves and a preparation method thereof are disclosed. The feed comprises the following raw materials, by weight, 300-400 parts of soyabean protein powder, 200-300 parts of corn bran, 200-300 parts of barley flour, 6-8 parts of soy sauce, 3-4 parts of mature vinegar, 2-3 parts of star aniseed powder, 2-3 parts of chilli sauce, 30-40 parts of pork jelly, 20-30 parts of fish gel, 70-80 parts of coagulated pig blood, 40-50 parts of coagulated duck blood, 20-30 parts of green pepper, 10-20 parts of onion, 10-15 parts of cottonseed oil, 50-60 parts of lagenaria siceria powder, 20-40 parts of mangosteen powder, 3-4 parts of Ligusticum wallichii, 2-3 parts of white peony root, 2-3 parts of Poria cocos, 1-2 parts of cortex lycii, 8-15 parts of coix seed oil, 5-10 parts of aloe freeze-dried powder, 3-5 parts of white carbon black, 50-60 parts of zoysia japonica, 40-50 parts of zoysia matrella, 20-30 parts of Cucurbita pepo L, 10-20 parts of spearmint, 5-10 parts of cypress leaf, 2-4 parts of gynostemma pentaphylla powder, 30-60 parts of rice bran, 20-30 parts of a phagostimulant and a proper amount of water.

The invention relates to a method for improving drought resistance of zoysia by utilizing salicylic acid. The adopted technical scheme is as follows: adopting a salicylic acid solution with the concentration of 0.1-1mM to perform foliage spraying; and spraying during the seedling stage of the zoysia, wherein the dates are often selected between May and June, the rainless weather is selected, the spraying time is 2-4 days, the spraying is performed 2-4 times per day, the spraying is often performed before 8:00am and after 5:00pm, and the spraying amount meets the standard of humidifying leaf blades without water dropping. By adopting the method disclosed by the invention for treating the zoysia, the drought resistance of the zoysia can be significantly improved; and furthermore, the method has the characteristics of simple process steps, easiness in operation, no production of secondary pollution and low cost, and is convenient for large-area treatment.

The invention discloses a young turtle fattening feed and a manufacturing method thereof. The young turtle fattening feed is prepared from hoof and horn meal, wool powder, dried locust powder, shrimp meal, coarse corn grains, sorghum, bone meal, green hyacinth beans, grapefruits, corn syrup, whole egg powder, zoysia matrella, nullipore, zoysia japonica, radix ophiopogonis, grist, semen coicis, ginkgo seeds, carrots, bengal waterdropwort herb, california burclover, white pepper, salt, agar, cotton stalks, edible bamboo charcoal powder and the like. The feed solves the problems that a traditional young turtle feed lacks nutrients and is poor in palatability, the manufactured feed is coarse, the young turtle is low in appetite, the weight of the young turtle is increased slowly, and the fattening effect is poor, the obtained feed is rich in nutrient, fine and smooth in taste, fragrant in taste, the appetite of the young turtle can be enhanced, weight increase and meat production are promoted, and the young turtle fed by the feed is high in appetite, rapidly gains weight, is good in fattening effect, can greatly improve economic benefits of young turtle culture and has the quite high popularization value.

The invention discloses a mealworm grass carp feed and a preparation method thereof. The mealworm grass carp feed is formed by mixing the following raw materials in parts by weight: 15-20 parts of mealworm powder, 1-3 parts of pea meal, 20-30 parts of corn flour, 1-3 parts of zoysia japonica powder, 2-4 parts of galium aparine powder, 4-8 parts of fresh cornstalk leaf powder, 3-6 parts of paspalum conjugatum powder, 2-5 parts of vinasse, 0.5-0.8 part of salt, 0.2-0.5 part of vinegar, 2-4 parts of honeysuckle flowers, 1-2 parts of radix puerariae, 0.3-0.5 part of large-head atractylodes rhizome and 0.8-1.2 parts of sodium bicarbonate. By the adoption of the scheme, a grass carp feed formula is scientific and reasonable, beneficial elements can be fully mixed, prepared feed particles are bulked to facilitate grass carp digestion, the requirement on the growth of grass carps in all stages can be met, the meat quality and the nutritive value of the grass carps are effectively improved, the growth of the grass carps is promoted, the grass carps are convenient to manage, the grass carp feed is convenient to prepare, and the cost of the feed is effectively controlled.

The invention discloses a novel method for suppressing Zoysia japonica endogenous gene expression efficiently and rapidly. Since Zoysia japonica genetic transformation is extremely difficult, carrying out Zoysia japonica gene function research and molecular breeding is restricted seriously. According to the method, Zoysia japonica endogenous gene expression is reduced specifically by agrobacterium-medated rice tungro bacillifrom virus gene silencing vectors, so that an efficient rapid technological means can be provided for gene functions of Zoysia japonica. The novel method includes the steps of 1), cloning target gene fragments of the Zoysia japonica; 2), constructing virus-induced gene silencing vectors; 3), impregnating Zoysia japonica plants by agrobacterium; 4), performing molecular detection to obtain genetic expression abundance. By the method, Zoysia japonica endogenous gene expression can be reduced by more than 30% successfully.

The invention relates to a method for improving drought resistance of zoysia. The adopted technical scheme is as follows: adopting a CaCl2 solution with the concentration of 5-20mM to perform foliage spraying; and spraying during the seedling stage of the zoysia, wherein the spraying time is 2-4 days, the spraying is performed 2-4 times per day, the spraying is often performed before 8:00am and after 5:00pm, and the spraying amount meets the standard of humidifying leaf blades without water dropping. By adopting the method disclosed by the invention for treating the zoysia, the drought resistance of the zoysia can be significantly improved; and furthermore, the method has the characteristics of simple process steps, easiness in operation, no production of secondary pollution and low cost, and is convenient for large-area treatment.

The invention provides an agrobacterium tumefaciens-mediated genetic transformation method of zoysia japonica. The method comprises the steps of infecting calluses of the zoysia japonica with agrobacterium tumefaciens suspended in a AAM-AS culture medium; putting the infected calluses in a N6D2-AS culture medium and co-culturing for 2-3 days; washing off the agrobacterium tumefaciens; screening and culturing the calluses. Aiming at three important influence factors which influence conversion efficiency of the agrobacterium tumefaciens-mediated genetic transformation system of the zoysia japonica, the best bacterium concentration (OD600 value being 0.3-0.4), infecting time (1.5-2 h) and co-culturing time (2-3 days) are found out. Combining with an improvement of the culture medium, final conversion efficiency reaches more than 50%. Besides, by improving washing method of the agrobacterium tumefaciens, pollutions of the agrobacterium tumefaciens can be eliminated in the screening and culturing process. Compared with previous agrobacterium tumefaciens-mediated genetic transformation methods, the method greatly reduces work-load, saves cost and prevents waste of callus materials.

The invention discloses a method capable of improving cold resistance zoysia japonica and belongs to the technical field of lawn planting. The method includes: subjecting zoysia japonica to potting orplug planting, culturing in an artificial weather culture box after growing for one month, and allowing illumination at 24 DEG C for 16h, wherein illumination intensity is 2000-3000umol.m-2.s-1; maintaining darkness at 4 DEG C for 8h, and culturing for 15-20d. The green period of zoysia japonica planted by the method can reach more than 220d in Northern regions (like Beijing and Lanzhou where annular minimum air temperature is higher than minus 20 DEG C), and zoysia japonica can winter safely.

The invention discloses a manufacturing method for salt-tolerant zoysia japonica pelleted seeds, belonging to the technical field of seed pretreatment. The manufacturing method comprises the followingsteps: carrying out seed dressing on healthy naked seeds with ascorbic acid, wherein the mass ratio of the naked seeds to the ascorbic acid is 1 g: (0.35-1.4) mg, and the solution concentration of the ascorbic acid is 2-8 mmol.L<-1>; allowing zoysia japonica seeds to roll in a pelleting machine at a constant speed, alternately adding an adhesive and a powder, and carrying out uniform stirring until the adhesive and the powder are uniformly coated on the zoysia japonica seeds so as to obtain the salt-tolerant zoysia japonica pelleted seeds. According to the invention, the ascorbic acid is adopted for seed dressing of the zoysia japonica seeds, so the salt stress effect of the zoysia japonica seeds can be effectively relieved; the vitality of the seeds can be improved; a certain germinationaccelerating effect is achieved; and the purposes of promoting seed germination, seedling uniformizing and seedling strengthening are achieved; meanwhile, the zoysia japonica seeds processed by pelleting are increased in volume and weight and uniform in specification, so mechanical and precise seeding can be easily realized.