Bipolaris panici-miliacei (Yi.Nisik.) Shoemaker (SX-5-2) and screening method and application thereof

A technology of Helmitosporium umbilicus and strains, which is applied in the prevention and control of crop weeds, and the microorganisms are applied to the field of agricultural plant protection, which can solve the problem of biological control of no Helminthosporium milletum, no Helminthia solani and its related species, etc. problem, to achieve the effect of not easy to be infected, low cost, strong specialization

Active Publication Date: 2017-12-01
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are domestic patents on the use of Helminthosporium genus Setaria and Helminthrospora as biological herbicides, but there is n

Method used

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  • Bipolaris panici-miliacei (Yi.Nisik.) Shoemaker (SX-5-2) and screening method and application thereof
  • Bipolaris panici-miliacei (Yi.Nisik.) Shoemaker (SX-5-2) and screening method and application thereof
  • Bipolaris panici-miliacei (Yi.Nisik.) Shoemaker (SX-5-2) and screening method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] The leaves of the diseased Azalea spp. were collected from the wild, the symptoms of the plants were recorded, and the microscopic examination was carried out. Isolation of pathogenic fungi was carried out on the agar medium of A. Pick single spores of each strain under a microscope, inoculate them on PDA medium, and cultivate them in the dark at 28°C. Observe the colony characteristics of pure culture of pathogenic fungi and measure the diameter of the colony. In addition, each colony disc with a diameter of 5 mm was inoculated in the center of the PDA plate, and the diameter of each colony was measured after 4 days.

[0063] Table 1 Comparison of the colony characteristics of the pathogenic fungi of Weeda mellifera

[0064]

[0065] Note: D1 represents the diameter of the colony formed after 5 days of single spores.

[0066] D2 represents the colony diameter formed after 4 days of 5mm colony disk.

Embodiment 2

[0068] The pathogenic fungus was collected and isolated from the diseased plants of Wetbrach chinensis naturally occurring in Zhejiang, and its pathogenicity was tested. Use different concentrations of various pathogenic fungal spore suspensions (wherein the surfactants are Tween 20, Tween 80, rapeseed oil and lecithin, and the stabilizers are antioxidants or pH regulators, including gelatin and gum arabic, etc.) , Spraying Attraction spp. under natural conditions to observe the pathogenic characteristics of each fungus.

[0069] Table 2 Comparison of the pathogenicity of several pathogenic fungi isolated from the plants of Athenia mellifera

[0070]

[0071] Note: 10 5 、10 4 Respectively, the sprayed spore suspension concentration is 10 per milliliter 5 、10 4 spores (the same below).

[0072] The lowercase letters are significant at the 0.05 level, and the uppercase letters are at the 0.01 significant level (the same below).

[0073] As can be seen from Table 2, the ...

Embodiment 3

[0075] The SX-5-2 strain was cultured on PDA medium for 4 days, placed under a black light at 28° C. for irradiation, and spore suspension was prepared with sterile water (same as Example 2). In addition, cultivate Attracta spp. seedlings in 9cm pots, and when they grow to the 3-4 leaf stage, spray the spore suspension with a hand-held sprayer, keep it moisturized for 48 hours at 28°C, and the humidity is 80%. After one week of treatment, the spore suspension can be completely killed. Damn it.

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Abstract

The invention discloses bipolaris panici-miliacei (Yi.Nisik.) Shoemaker (SX-5-2) and a screening method and application thereof. The bipolaris panici-miliacei (Yi.Nisik.) Shoemaker (SX-5-2) has been preserved in the general microbiological center of Chinese microbial species preservation management committee, and the preservation number is CGMCC No.13887. The concentration of conidium which utilizes the bipolaris panici-miliacei (Yi.Nisik.) Shoemaker (SX-5-2) is more than 104 per milliliter, and the bipolaris panici-miliacei (Yi.Nisik.) Shoemaker (SX-5-2) is used for biological weeding, can be safely used in Oryza sativa L., Triticum aestivum L., sorghum bicolor (L.) Moench, Glycine max (Linn.) Merr., Gossypium spp and vegetable crop fields as well as Festuca elata Keng ex E. Alexeev and Zoysia japonica Sreud., and is used for controlling gramineous weeds such as Digitaria sanguinalis (L.) Scop, Eleusine indica (L.) Gaertn, Setaria viridis (L.) Beauv., Echinoochloa crusgalli (l.) Beauv., Leptochloa chinensis (L.) Nees, Microstegium vimineum (Trin.) A. Camus, Alopecurus aequalis Sobol. and Poa annua L., as well as microstegium related genera plant such as Eccoilopus cotulifer (Thunb.) A., Miscanthus sinensis anderss., Eulalia speciosa, Microstegium japonicum, Arthraxon hispidus (Thunb.) makino, Sorghym sudanense (Piper stapf). The controlling effect on target weeds can reach more than 80%. The bipolaris panici-miliacei (Yi.Nisik.) Shoemaker (SX-5-2) has the characteristics of safety and environmental protection.

Description

technical field [0001] The invention belongs to the technical field of microorganisms applied to agricultural plant protection and crop weed control, and specifically relates to a strain of Helminthrospora umbilicalis Bipolaris panici-miliacei (Y.Nisik.) Shoemaker (SX-5-2), its screening method and its use. Background technique [0002] my country is a large agricultural country with a population of 1.4 billion. However, my country's food production needs to be supplemented by foreign imports. The extensive use of chemical pesticides and the frequent occurrence of weeds in crop fields seriously restrict the development of modern agriculture. There are 255 species of farmland weeds in my country, of which about 10 species occur in a large area, and among these 10 species, gramineous weeds account for 40%. Goosegrass, foxtail, barnyardgrass, daughter-in-law, bermudagrass, sagegrass, crabgrass, chrysanthemum, bluegrass, bluegrass, etc. are important farmland grass weeds in my ...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12Q1/68C12Q1/04A01N63/04A01P13/00C12R1/645
CPCA01N63/30C12Q1/6895C12N1/145C12R2001/645
Inventor 强胜丁如意黄琼
Owner NANJING AGRICULTURAL UNIVERSITY
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