Regeneration method of Japanese lawngrass

A technology of zoysia and regeneration medium, which is applied in the field of plant tissue culture, and can solve problems such as high difficulty, low regeneration frequency, and difficulty in maintaining callus embryogenicity

Inactive Publication Date: 2007-08-15
BEIJING FORESTRY UNIVERSITY +2
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Problems solved by technology

However, the in vitro culture of Zoysia japonica is more difficult than that of cool-season turfgrass. Although there have been reports on the establishment of a tissue culture reg...

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  • Regeneration method of Japanese lawngrass
  • Regeneration method of Japanese lawngrass

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Embodiment Construction

[0022] The following examples are used to further illustrate the present invention, but are not intended to limit the scope of the present invention.

[0023] 1. Materials and methods

[0024] 1.1 Test material:

[0025] Japanese Zoysia species: 'Zenith'(Zoysia japonica cv.Zenith),'Liaoning'(Zoysia japonica cv.Liaoning),'Qingdao'(Zoysia japonica cv.Qingdao),'Lanyin 3'(Zoysia japonica cv.Lanyin3) The seeds of 'Zenith' were purchased from American TMI Company (Turf Merchants, Inc), the seeds of 'Liaoning' and 'Qingdao' were purchased from Beijing Seed Company, and the seeds of 'Lanyin 3' were purchased from the Institute of Agriculture and Animal Husbandry, Chinese Academy of Tropical Agricultural Sciences.

[0026] 1.2 Obtaining of explants

[0027] Soak the mature seeds in about 50 mL of sodium hypochlorite solution (produced by Beijing Chemical Factory, active chlorine> 5%, add 1-2 drops of Tween 20), sterilize on a magnetic stirrer for 1 hour, rinse with sterile water 3-5 ...

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Abstract

The invention provides a method for regenerating Japanese lawngrass. It comprises pretreating explant, inoculating callus inducing culture medium and getting callus; selecting embryogenic callus and inoculating it in subculture medium for subculture, or inoculating regenerative culture medium for regenerative plant differentiation; the callus inducing culture medium contains 2, 4- D 3- 6mg/l, 6- BA or KT 0.05- 0.2 kg/l and CuSO4 2- 3.5 mg/l. The inducing rate for explant callus can reach 90%, embryogenic callus inducing rate can reach 30%, and the regenerating rate for callus can reach 45%. The regenerative plant is characterized by strong tillering and rooting ability, high survival rate of plant after strong seedling and hardening seedling, which reaches above 87.5%. The replanted plant can propagate in field normally.

Description

technical field [0001] The invention relates to plant tissue culture technology, in particular to a high-frequency regeneration method of Zoysia japonica. Background technique [0002] Zoysia japonica (Poa pratensis L.) is a high-quality warm-season turfgrass of Poaceae. Using genetic engineering to improve varieties and breed new varieties of Zoysia japonica has become the focus of current research in the field of Zoysia pratensis biotechnology. The receptor system for gene transformation of Zoysia japonica is mainly derived from tissue culture. As a receptor system for gene transformation, it is required to have strong regeneration ability, high genetic stability, stable source of explants, and selective Sensitivity to antibiotics, sensitivity to Agrobacterium infection, etc. Therefore, establishing an efficient regeneration system has always been the goal pursued by Japanese Zoysia breeders. However, the in vitro culture of Zoysia japonica is more difficult than that of...

Claims

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Application Information

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IPC IPC(8): A01H4/00C12N5/04
Inventor 韩烈保齐春辉梁小红方文娟曾会明
Owner BEIJING FORESTRY UNIVERSITY
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