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Microsclerotium bacterial strain and method for biological weed control therewith

A technology of Sclerotinia strains, Sclerotinia microbes, applied in the direction of microorganism-based methods, chemicals for biological control, biochemical equipment and methods, etc.

Active Publication Date: 2010-05-26
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In China, there is no application for the genus Sclerotium, especially Sclerotium rolfsii (SC64), and it is useless for biological weeding of phytopathogens in lawns, golf courses, parks and gramineous crop fields to control broad-leaved weeds agent

Method used

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  • Microsclerotium bacterial strain and method for biological weed control therewith
  • Microsclerotium bacterial strain and method for biological weed control therewith
  • Microsclerotium bacterial strain and method for biological weed control therewith

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0105] Example 1: Sclerotinia sclerotiorum was collected and isolated from the diseased plants of Solidago canadensis naturally occurring in Nanjing, and its pathogenicity was tested.

[0106] The stems and roots of diseased Solidago canadensis were collected from the wild, the symptoms of the plants were recorded, and microscopic examination was performed. Isolation of pathogenic fungi was carried out on solidago canadensis sap agar medium. Pick the single sclerotia of the strain, inoculate them on PDA medium respectively, and cultivate them in the dark at 28±1°C, observe the colony characteristics of the pure culture of pathogenic fungi and measure the diameter of each colony.

[0107] The pathogenicity of different Sclerotinia microbes to broad-leaved weeds was compared under greenhouse conditions. Sc085 was isolated from begonia and Sc087 was isolated from peanut. The weeds tested included Conyza canadensis (L.) Cronq., Sonchus asper (L.) Hill), Vicia sativa L., Veronica...

Embodiment 2

[0110] Example 2: After mixing the solid matrix of cottonseed hulls with water according to 1:0.6, routinely sterilize, inoculate the SC64 bacterial classification on the edge of PDA with vigorous growth after cooling, turn over the medium every day, and ferment the bacteria granules after the hyphae are overgrown. Dry the herbicidal bacteria particles in a fume hood and use them in the test in time, and use the sterilized cottonseed hull matrix as a control. The Sclerotinia microbe strain SC64 was tested indoors against Channa sausage (4th true leaf stage), lettuce (4th leaf stage), purslane (4th to 5th true leaf stage), Solidago canadensis (6th leaf stage) ) and Moshangcai (early flowering stage) and other target weeds, the temperature during the test period was 22-30°C.

[0111] Table 4 Control effect of Sclerotinia microbe strain SC64 on several main target weeds

[0112]

[0113] Note: The lowercase letters are significant at the 0.05 level, and the uppercase letters ...

Embodiment 3

[0114] Example 3: According to the method of Example 2, the pathogenicity of different solid fermented herbicidal bacterial granules of Sclerotinia microspermum strain SC64 on the true leaf ages of 2-4 pairs of Channa sausis was tested.

[0115] Table 5 Pathogenic effect of different solid fermented herbicide granules of strain SC64 on snakehead sausage

[0116]

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Abstract

The invention relates to a microsclerotium bacterial strain and a method for biological weed control therewith, belonging to the application of microorganism for agricultural plant protection. The microsclerotium bacterial strain is (SC64) CGMCC with number thereof being NO.2934. The use dosage of weeding bacterial particles obtained by solid fermentation of the bacterial strain ranges from 30g.m-2-150 g.m-2, and the weeding bacterial particles can be applied to poaceae crops such as corn, rice, wheat, barley, sugar cane, kaoliang, millet and poaceae plants such as lawn bermuda grass, zoysia japonica, festuca arundinacea, poa annua, agrostis stolonifera, axonopus compressus, eremochloa ophiuroides, ditch millet to control growth of broad-leaf weed. In addition, in particular, the weeding bacterial particles can be applied to control destructive invasive foreign weeds such as eupatorium adenophorum, solidago canadensis, horseweed herb, conyza sumatrensis, flaveria bidentis, mikania micrantha, annual fleabane herb and fragrant eupatorium herb. 80% of the target weeds can be brought under control, thus having the characteristics of safety and environmental friendliness.

Description

technical field [0001] The invention relates to a Sclerotinia sclerotiorum strain and a method for biological weeding thereof, belonging to the technical field of microorganisms applied to agricultural plant protection and control of crop weeds, and is specially used for the control of broad-leaved weeds in gramineous crop fields and lawns. Background technique [0002] With the enhancement of people's ecological awareness, urban and rural greening construction has developed rapidly in recent years, especially the green area dominated by gramineous lawns has continued to expand. Many broadleaf weeds, including Sumen white winegrass (Conyza sumatrehsis (Reez) Walker), dandelion (Taraxacum mongolicum Hand.-Mazz.), annual cape (Erigeron annuus (L.) segetum (Bunge) Kitam.), Chickweed (Malachium aquaticum (L.) Fries), Veronica persica Poir., Plantago asiatica L., Vicia sativa L. , Ranunculus sceleratus L., Cardamine hirsuta L. and sedge (yperus difformis L.) broad-leaved weeds i...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P13/02C12R1/01
Inventor 强胜唐伟朱云枝陈世国
Owner NANJING AGRICULTURAL UNIVERSITY
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