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Method used for inducing callus with Zoysia leaf base

A technology for callus and Zoysia, applied in the field of tissue culture of Zoysia plants, can solve the problems of small collection, difficult differentiation and proliferation, low callus induction rate and the like, and achieves high induction rate, long time effect

Inactive Publication Date: 2016-12-21
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Zoysia Manila and Zoysia sinensis are Zoysia plants that are widely used in my country at present. However, most lawn grasses are monocotyledonous herbaceous plants, and their differentiation and proliferation are not so easy. Zoysia is a typical warm-season lawn grass. , it is difficult to induce callus, which makes it difficult to perform tissue culture plant regeneration and genetic transformation based on callus induction
[0003] At present, although there are also reports of successful callus induction of Zoysia plants, the selected explants are mainly stolons. Compared with other parts of plants, the number of stolons of Zoysia plants that can be collected is small, and they can only be collected under high temperature and high humidity. season, with appropriate expansion, and callus induction is limited to new stolons, the time and quantity of explants available for collection are greatly limited, and the callus induction rate is low

Method used

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  • Method used for inducing callus with Zoysia leaf base

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Select Zoysia Manila to induce callus, the method is as follows:

[0021] (1) Preparation of medium: select MS medium as the base medium, add sucrose and agar, the concentrations are 30g / L and 7g / L respectively; then add 6-benzylaminoadenine and 2,4-dichlorobenzene Oxyacetic acid, the concentration of which is 0.1mg / L and 2.0mg / L respectively; adjust the pH value to 5.8, and sterilize under high pressure at 121°C for 25min to obtain it;

[0022] (2) Explant treatment: choose healthy and disease-free plants of the genus Zoysia to plant upright, take mature leaves from the top to the base, and take 1-4 healthy leaves from the top, rinse with pure water first, and then soak with washing powder and water 20 minutes, then rinse with pure water for 20 minutes; disinfection: first soak in 95% alcohol for 30s, then soak in 0.1% mercury liter for 3 minutes, and finally rinse with sterile water for 5 times;

[0023] (3) Pruning: cut off the base of the blade, that is, cut off th...

Embodiment 2

[0026] Select Zoysia sinensis to induce callus, the method is as follows:

[0027] (1) Preparation of medium: select MS medium as the base medium, add sucrose and agar, the concentrations are 30g / L and 7g / L respectively; then add 6-benzylaminoadenine and 2,4-dichlorobenzene Oxyacetic acid, the concentration of which is 0.1mg / L and 3.0mg / L respectively; adjust the pH value to 5.8, and sterilize under high pressure at 121°C for 25min to obtain it;

[0028] (2) Explant treatment: choose healthy and disease-free plants of the genus Zoysia to plant upright, take mature leaves from the top to the base, and take 1-4 healthy leaves from the top, rinse with pure water first, and then soak with washing powder and water 20 minutes, then rinse with pure water for 20 minutes; disinfection: first soak in 95% alcohol for 30s, then soak in 0.1% mercury liter for 5 minutes, and finally rinse with sterile water for 5 times;

[0029] (3) Pruning: cut off the base of the blade, that is, cut off ...

Embodiment 3

[0032] Select Zoysia sinensis to induce callus, the method is as follows:

[0033] (1) Preparation of medium: select MS medium as the base medium, add sucrose and agar, the concentrations are 30g / L and 7g / L respectively; then add 6-benzylaminoadenine and 2,4-dichlorobenzene Oxyacetic acid, the concentration of which is 0.1mg / L and 2.5mg / L respectively; adjust the pH value to 5.8, and sterilize under high pressure at 121°C for 25min to obtain it;

[0034] (2) Explant treatment: choose healthy and disease-free plants of the genus Zoysia to plant upright, take mature leaves from the top to the base, and take 1-4 healthy leaves from the top, rinse with pure water first, and then soak with washing powder and water 20 minutes, then rinse with pure water for 20 minutes; disinfection: first soak in 95% alcohol for 30s, then soak in 0.1% mercury liter for 4 minutes, and finally rinse with sterile water for 5 times;

[0035] (3) Pruning: cut off the base of the blade, that is, cut off ...

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Abstract

The invention discloses a method used for inducing callus with Zoysia leaf base. The method comprises following steps: 1, a culture medium is prepared; 2, explants are treated, 3, cutting is carried; and 4, inoculated culture is carried out. Compared with the prior art, the method is used for inducing callus via taking leaf base as the explants, and possesses following advantages: 1, many leaves are available, so that the amount of the explants to be collected is large; 2, the explants are available for a long term; and 3, callus induction efficiency is high.

Description

technical field [0001] The invention relates to a method for inducing callus by utilizing the leaf base of plants of the genus Zoysia, and belongs to the technical field of tissue culture of plants of the genus Zoysia. Background technique [0002] As a warm-season turfgrass plant widely distributed in the world, Zoysia has the advantages of trampling resistance, drought resistance, strong comprehensive adaptability, resistance to extensive management, and ecological environmental protection. Zoysia Manila and Zoysia sinensis are Zoysia plants that are widely used in my country at present. However, most lawn grasses are monocotyledonous herbaceous plants, and their differentiation and proliferation are not so easy. Zoysia is a typical warm-season lawn grass. , it is difficult to induce callus, which makes it difficult to carry out tissue culture plant regeneration and genetic transformation based on callus induction. [0003] At present, although there are also reports of su...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/005
Inventor 方敏彦章明李祖祥汪善锋李园莉
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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