Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

High-efficiency gene editing method of Zoysia japonica mediated by gene gun

A technology of gene editing and zoysia, which is applied in the fields of genetic engineering and plant genetic breeding, can solve problems such as the difficulty of quantitative trait loci linkage relationship resistant varieties

Active Publication Date: 2021-12-24
BEIJING FORESTRY UNIVERSITY
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Turfgrass breeders all over the world have been committed to the cultivation of new varieties of Zoysia japonica for many years, but most of them adopt traditional breeding methods. Due to the complexity of plant stress resistance itself and the complexity of the linkage relationship of its quantitative trait loci It is very difficult to obtain excellent resistant varieties by traditional breeding methods, and the use of genetic engineering technology to obtain turfgrass varieties adapted to or resistant to adverse environmental factors has become one of the quick and effective methods to solve this problem

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • High-efficiency gene editing method of Zoysia japonica mediated by gene gun
  • High-efficiency gene editing method of Zoysia japonica mediated by gene gun
  • High-efficiency gene editing method of Zoysia japonica mediated by gene gun

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Gene gun-mediated high-efficiency gene editing method for Zoysia japonica

[0030] 1 Materials and methods

[0031] 1.1 Plant material

[0032] Mature seeds of Japanese Zoysia species "Chinese common" (purchased from Beijing Zhengdao Ecological Technology Co., Ltd.) were sterilized by soaking in 70% alcohol for 1 minute, rinsed with distilled water for 3 times, and washed with 30% sodium hypochlorite solution (produced by Beijing Chemical Factory, >5% Active chlorine, add a drop of Tween-20) to sterilize on a magnetic stirrer for 1 hour, rinse with sterile water 3-5 times, soak at 4°C for 3 days, then sterilize with sodium hypochlorite solution for 20 minutes, rinse with sterile water for 3 After the second time, blot the excess water with sterile filter paper, inoculate on the callus induction medium, and induce callus at 28°C in a dark state. The callus is subcultured once a month, and white or yellow compact granular The callus was transferred to the subc...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention provides a gene gun-mediated high-efficiency gene editing method for Zoysia japonica, designing an sgRNA sequence based on CRISPR / Cas9 for the target gene ZjSGR in Zoysia japonica, and connecting the DNA fragment containing the coding sgRNA sequence to a gene carrying CRISPR In the vector of CRISPR / Cas9, Zoysia japonica is transformed by the gene gun-mediated transformation method, or the vector containing the DNA fragment encoding the sgRNA sequence, the vector carrying CRISPR / Cas9 and the vector containing the selection marker gene are transformed by the gene gun-mediated transformation method. The transgenic Zoysia japonica plants were co-transformed with the inductive transformation method to realize the site-directed mutation of the Zoysia japonica gene ZjSGR, and then to obtain the transgenic Zoysia japonica plants lacking the function of the gene. The invention provides a gene gun-mediated high-efficiency gene editing method of Zoysia japonica, as a tool for directional editing of Zoysia japonica genes, and the editing efficiency is as high as 37.5%.

Description

technical field [0001] The invention relates to the fields of genetic engineering and plant genetic breeding, in particular to a gene gun-mediated high-efficiency gene editing method for Zoysia japonica. Background technique [0002] Japanese Zoysia (Zoysia japonica Steud.) is the only high-quality warm-season turfgrass of Gramineae that is rich in resources in my country and does not need to be imported. It has strong adaptability, drought resistance, heat resistance, cold resistance, trampling resistance and barren resistance. Characters play an important role in modern urban greening, sports field construction and soil and water conservation. Turfgrass breeders all over the world have been committed to the cultivation of new varieties of Zoysia japonica for many years, but most of them adopt traditional breeding methods. Due to the complexity of plant stress resistance itself and the complexity of the linkage relationship of its quantitative trait loci It is very difficul...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/89C12N15/82C12N15/113C12N9/02A01H5/00A01H6/46
CPCC12N15/895C12N15/8218C07K14/415C12N15/113C12N9/22
Inventor 高娅楠许立新张娜李丽菁张智韦
Owner BEIJING FORESTRY UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com