Molecular marking method for identifying variety of Suzhi No.1 hybrid zoysia

A molecular marker and variety identification technology, applied in the field of plant genetic markers and variety molecular identification, can solve the problems that developers and users are difficult to distinguish the authenticity of varieties and the influence of new varieties development and utilization, and achieve high repeatability and reliable identification Effect

Active Publication Date: 2015-07-15
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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Problems solved by technology

[0004] 'Suzhi 1' hybrid Zoysia is a typical new vegetative propagation Zoysia species. Its external shape is easily affected by the natural ecological conditions of growth and the level of lawn management, which makes it difficult for developers and users to identify the species. The authenticity of the new varieties will have an impact on the further development and utilization of new varieties

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  • Molecular marking method for identifying variety of Suzhi No.1 hybrid zoysia
  • Molecular marking method for identifying variety of Suzhi No.1 hybrid zoysia
  • Molecular marking method for identifying variety of Suzhi No.1 hybrid zoysia

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Embodiment Construction

[0029] Utilize SRAP molecular marker technology to distinguish ' Su Zhi No. 1 ' hybridization Zoysia among the present invention and take as follows:

[0030] (1) Extraction of DNA and acquisition of identification markers

[0031] A new broad-spectrum plant genomic DNA rapid extraction kit (HF213) was used to extract DNA from the leaves of Zoysias hybrida 'Suzhi 1'.

[0032] Use 48 pairs of SRAP primers to amplify the extracted DNA by PCR. SRAP-PCR uses a 10 μL reaction system, which includes: 50ng template DNA, 10×PCR buffer, Mg 2+ 1.5mmol·L -1 , dNTPs200μmol·L -1, primer 0.2 μmol L -1 , Taq DNA polymerase 0.5U. The SRAP-PCR amplification program was: 94°C pre-denaturation for 4 min; 94°C denaturation for 1 min, 37°C annealing for 1 min, 72°C extension for 10 sec, 5 cycles; 94°C denaturation for 1 min, 50°C annealing for 1 min, 72°C extension for 10 sec, 35 cycles Cycle; extend at 72°C for 7 min after cycle, and store at 4°C. The amplification reaction was carried out ...

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Abstract

The invention relates to a method for molecularly identifying the genetic markers and the variety of a plant, and specifically discloses a group of SRAP (Sequence-Related Amplified Polymorphism) marking primers and an identification method for identifying Suzhi No.1 hybrid zoysia. The group of primers is used for PCR (Polymerase Chain Reaction) to obtain the amplified fragments of each pair of primers; the obtained fragments are compared with genome fragments disclosed by the invention; consequently, whether the zoysia variety is the Suzhi No.1 hybrid zoysia can be identified accurately.

Description

1. Technical field [0001] The invention relates to a plant genetic marker and a method for molecular identification of varieties, in particular to a method for molecular identification of 'Suzhi No. 1' hybrid Zoysia species based on genome SRAP analysis. 2. Technical background [0002] Zoysia (Zoysia Willd.) is a perennial warm-season turfgrass that is widely distributed in the coastal areas of the west and north coasts of the Pacific Ocean, and is also distributed in warm regions such as Africa and Australia. my country is the country with the widest distribution and largest reserves of zoysia in the world. However, from the perspective of the cultivation of new varieties, my country is far behind the United States, Japan and South Korea. The United States does not produce Zoysia grass, but when Americans discovered that Zoysia grass was an excellent lawn grass, they introduced Zoysia grass from abroad in large quantities. Texas A&M University (Texas A&M University) More...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6895C12Q2600/156
Inventor 郭海林刘建秀郭爱桂陈静波宗俊勤
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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