Application of plant sodium-calcium exchanger gene (AtNCL)

A sodium-calcium exchange and protein technology, which is applied in the field of genetic engineering, can solve the problem of no coding gene for cloning sodium-calcium exchange active protein, etc., and achieve the effect of improving salt tolerance

Inactive Publication Date: 2011-01-26
HEBEI NORMAL UNIV
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  • Abstract
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Problems solved by technology

However, no gene encoding a protein with such sodium-calcium exchange activity has been cloned in plants

Method used

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  • Application of plant sodium-calcium exchanger gene (AtNCL)
  • Application of plant sodium-calcium exchanger gene (AtNCL)
  • Application of plant sodium-calcium exchanger gene (AtNCL)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Gene acquisition

[0024] A small amount of RNA was extracted from leaves of wild-type Col-0. The glassware used in the experiment was dry-baked at 180°C for 7-10 hours, and the water, plastic products, and solutions were all treated with DEPC and then sterilized by high pressure. Follow the steps in the instructions of the RNARose kit of Shanghai Huashun Bioengineering Co., Ltd. Grind about 100mg of tissue with liquid nitrogen, put it in a centrifuge tube, add 1ml RNARose to shake and mix well, let stand at room temperature for 5-10min; Mix well in the centrifuge tube and let stand for 5-10min. After layering, centrifuge at 12000g for 15min at 4°C; carefully transfer the upper aqueous phase to a new centrifuge tube, add an equal volume of isopropanol to mix gently, and place at room temperature for 5-10min , centrifuge at 12000g at 4°C for 10min; remove the supernatant carefully, add 1ml of 75% ethanol to shake for a while, centrifuge at 7500g at 4°C for 5min, remove...

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Abstract

The invention discloses a plant sodium-calcium exchanger gene (AtNCL) and application of the sodium-calcium transport activity of the coding protein thereof in breeding salt-resistant variety of crop. The number of a model plant Arabidopsis thaliana sodium-calcium exchanger gene (AtNCL) in an Arabidopsis thaliana genome database (TAIR) is Atlg53210; the total length of the open reading frame of the gene is 1,758bp, and protein consisting of 585 amino acid residues is coded. Experiments prove that AtNCL participates in the elimination process of calcium signals of plant response to salt stress, and that a new ion transport system exists in the plant. By regulating the expression of the plant sodium-calcium exchanger gene through the crop transgenic technology according to the characteristics of AtNCL, a new crop variety with improved salt resistance can be obtained.

Description

technical field [0001] The invention relates to the field of genetic engineering, in particular to the application of a plant sodium-calcium exchange protein gene (AtNCL) and the sodium-calcium transport activity of its coded protein in breeding salt-tolerant varieties of crops. Background technique [0002] Plants have certain adaptability and resistance to external environmental changes and even stress stimuli. This adaptability is accomplished through the physiological and biochemical mechanisms of individual plants. The elucidation of the mechanism of plant stress resistance not only has important theoretical significance, but also has important application value. The study of plant salt tolerance is one of the hotspots. Breeding salt-alkali-tolerant crops with traditional genetic breeding methods is difficult and takes a long time, and it is difficult to meet the display needs of our country. With the development of molecular biology, molecular breeding began to come ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C07K14/415A01H5/00
Inventor 崔素娟孙大业王鹏李朝炜
Owner HEBEI NORMAL UNIV
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