Method for testing microbial activity of artificial wet land sewage treatment system
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A sewage treatment system and microbial activity technology, applied in biochemical equipment and methods, microbial determination/inspection, electrical digital data processing, etc., to avoid incomplete extraction, simplify operation steps, and ensure health.
Active Publication Date: 2008-08-27
SHANGHAI MUNICIPAL ENG DESIGN INST GRP
View PDF0 Cites 3 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
[0018] The purpose of the present invention is to provide a method for detecting microbial activity in constructed wetlands, which solves the above-mentioned deficiencies in the existing methods for detecting the activity of microbial electron transfer systems in constructed wetland sewage treatment systems, and achieves rapid, accurate and safe detection of the activity of microbial electron transfer systems in constructed wetlands. the goal of
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0061] (1) Take out the plant roots attached to microorganisms from the constructed wetland sewage treatment system, first ultrasonically vibrate them for 10 minutes, then collect the fallen biofilms, and dilute them with sterile water for later use;
[0062] (2) Clean the biological activity test tube with sterile water, dry it at (105±1)°C to constant weight, and record the mass W 1 ;
[0063] (3) Add 1 mL of microbial suspension, 1.5 mL of Tris-HCl buffer solution with a pH of 8.4 and 1 mL of INT solution with a weight concentration of 2 mg / mL in a 10 mL test tube;
[0064] (4) Place the prepared sample in a water bath shaker at (37±1)°C, and shake it in the dark for 2 hours;
[0065] (5) After the cultivation process is over, add 1mL of formaldehyde solution with a volume concentration of 37% to the test tube to terminate the enzyme reaction, then centrifuge the sample at 4000r / min for 5min, and discard the supernatant;
[0066] (6) Add 5 mL of ethanol to the sample, mix...
Embodiment 2
[0093] (1) Take out the wetland substrate with attached microorganisms from the constructed wetland sewage treatment system, first vibrate it ultrasonically for 5 minutes, then collect the fallen biofilm, and dilute it with sterile water for later use;
[0094] (2) Clean the biological activity test tube with sterile water, dry it at (105±1)°C to constant weight, and record the mass W 1 ;
[0095] (3) Add 1 mL of microbial suspension, 2 mL of Tris-HCl buffer solution with a pH of 8.4 and 2 mL of INT solution with a weight concentration of 2 mg / mL in the test tube of 10 mL;
[0096] (4) Place the prepared sample in a water bath shaker at (37±1)°C, and shake it in the dark for 1 hour;
[0097] (5) After the cultivation process is over, add 1mL of formaldehyde solution with a volume concentration of 37% to the test tube to terminate the enzyme reaction, then centrifuge the sample at 4000r / min for 5min, and discard the supernatant;
[0098] (6) Add 5 mL of ethanol to the sample,...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Login to view more
Abstract
The present invention provides a microorganism activity detection method for constructed wetland sewage treatment system, comprising the following basic steps of constructed wetland microorganism sampling, cultivation, extraction, measurement and calculation. The present invention adopts iodine nitro group tetrazolium as an electron acceptor, cancels oxygen removal step on the basis of the strongelectron affinity of INT, adopts 2h as the cultivation time, and uses ethanol as extractant to extract INTF inside microorganism cells. The detection method has the advantages that: the INT is strongin electron affinity and the ability of competing with oxygen for electrons; the present invention can be used to detect the electron transport system activity of aerobic, anaerobic and denitrification nitrogen removal microorganisms in constructed wetland systems, finishes extraction process under the condition of normal temperature, and avoids incomplete extraction, extractant evaporation and other problems which are caused by high temperature; the microorganism dry weight direct determination method is combined with the detection process of microorganism electron transport activity, thereby avoiding errors caused by different matrixes and plant rhizosphere biomass as well as uneven sampling.
Description
technical field [0001] The invention relates to a method for detecting microbial activity, in particular to a method for detecting microbial activity in a sewage treatment system. Background technique [0002] The metabolic activity of microorganisms in the constructed wetland sewage treatment system is the key to maintaining the long-term purification effect of the constructed wetland, and microorganisms play an important role in the process of organic matter degradation and denitrification. [0003] Microbial activity is an important index to characterize the strength of microbial metabolism in constructed wetland system. This index can not only reflect the ability of microorganisms to remove pollutants in constructed wetland sewage treatment system from the perspective of molecular biology, but also measure the biological activity of pollutants from the level of biological activity. Degradation rate and evaluation and prediction of operating effect of constructed wetland ...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to view more 
Login to view more