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Method for identifying animal-based components in meat and meat products by utilizing SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis) method

A technology of animal origin and meat products, which is applied in the field of food hygiene inspection and can solve problems such as the difficulty in identifying animal origin ingredients

Inactive Publication Date: 2011-10-12
QINGDAO AGRI UNIV +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to solve the problem of difficult identification of animal-derived components in food safety and sanitation inspection, and to provide a method for identifying pig, cattle, sheep, chicken, and fish-derived ingredients in meat and meat products using SDS-polyacrylamide gel electrophoresis technology. ingredient method

Method used

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  • Method for identifying animal-based components in meat and meat products by utilizing SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis) method
  • Method for identifying animal-based components in meat and meat products by utilizing SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis) method
  • Method for identifying animal-based components in meat and meat products by utilizing SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis) method

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Embodiment 1

[0029] (1) Collection of samples: Randomly collect samples of pigs, cattle, sheep, chickens, and carps in the market, totaling 5 samples.

[0030] (2) Sample preparation: Weigh the skinless muscle tissue, about 2g each (2cm×1cm×1cm), 7 copies in total, one of which is kept at room temperature, and the remaining 6 are placed at 50°C, 60°C, and 70°C respectively , 80°C, 90°C, 100°C in a constant temperature water bath for 3 minutes, immediately take it out and put it in a mortar, add 20ml of 0.5M Tris-HCl buffer solution pre-cooled at 4°C, grind and homogenate for 10 minutes until the solution is thin, Transfer 1ml of the processed sample, centrifuge at 8000r / min for 15min, absorb 100μL of centrifuged supernatant, which is the soluble protein, and use this sample as the test sample.

[0031] (3) SDS-polyacrylamide gel electrophoresis:

[0032] Glue production: the concentration of separating gel is 12%, the concentration of stacking gel is 5%, and the thickness is 1mm.

[0033...

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Abstract

The invention relates to a method for identifying the animal-based components in meat and meat products by utilizing an SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis) method, and belongs to the field of food sanitation inspection. The method provided by the invention is utilized to identify the pig, cattle, sheep, chicken and fish-based components in the meat products; the SDS-PAGE is carried out on the soluble proteins which are extracted from the processed sample by carrying out different temperature thermal treatments on fresh animal muscle tissues; and the pig, cattle, sheep, chicken and fish-based components in the meat products are judged according to an electrophoresis pattern. The method provided by the invention has the characteristics of strong specificity and sensitivity, and visual and reliable result judgment, and is simple to operate and has the extremely important significances on preventing animal disease pathogens such as foot-and-mouth disease, avian influenza and the like from spreading into China, preventing illegal retailers from concealing commodity components and guaranteeing the health of people.

Description

1. Technical field [0001] The invention relates to a method for identifying animal-derived components in meat and meat products by using SDS-polyacrylamide gel electrophoresis, and using SDS-polyacrylamide gel electrophoresis to identify pigs, cattle, sheep, Chicken and fish-derived ingredients belong to the field of food hygiene inspection and are of great significance in food hygiene and safety inspection. 2. Background technology [0002] Meat products have high nutritional value, contain high-quality protein and a variety of essential amino acids, and are important foods in the human diet. With the continuous change of people's dietary structure, the demand for meat is increasing, and there are many counterfeit mutton or beef in the market. It became "mutton roll" and "beef roll". Due to the discovery and prevalence of diseases such as mad cow disease, foot-and-mouth disease, and bird flu, the animal-derived ingredients in food are strictly restricted, and many people ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/561G01N33/12
Inventor 孙京新黄明周光宏徐幸莲罗欣董佩谕
Owner QINGDAO AGRI UNIV
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