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107results about How to "Prevent incoming" patented technology

Method for identifying source constituent in meat product by micro-satellite labeling technique

The invention relates to a method which uses microsatellite marking technology for identifying the animal origin ingredients in meat products, belonging to the biological high-tech field. The use of the microsatellite marking technology for identifying the origin ingredients of cattle, sheep, pigs, dogs, chickens and ducks in the meat products comprises the extraction of genomic DNA in meat products, the conducting of PCR reaction, the PCR product detection and primer screening, and the identification of samples. The method selects microsatellite marks with specific property among the types and universal property in the type, designs specific primers, establishes PCR detection methods, and identifies the origin ingredients of cattle, sheep, pigs, dogs, chickens and ducks in the meat products according to the size and the availability of amplification fragment. The invention has the advantages of rapidity, simplicity, accuracy, strong specificity and high sensitiveness. The invention is suitable for being promoted and used for detecting multitudinous samples. The invention also has great significance for preventing the mad cow disease from being spread into China and the illegal businessmen from concealing the commodity elements, and ensuring national security and the health of people.
Owner:NANJING AGRICULTURAL UNIVERSITY

Monoclonal antibody for African swine fever virus gene II type strain as well as preparation method and application thereof

The invention discloses a monoclonal antibody for an African swine fever virus gene II type strain as well as a preparation method and application thereof. The preparation method of the monoclonal antibody for the African swine fever virus gene II type strain comprises the following steps: immunizing a mouse by adopting a purified p54 immune protein, fusing spleen cells of the immunized mouse with myeloma cells to prepare hybridoma cells, screening with three screening antigens so as to obtain the hybridoma cells which can stably secrete a monoclonal antibody, and then preparing the monoclonal antibody by adopting an in vivo or in vitro method, wherein the three screening antigens comprise a prokaryotically expressed p54 recombinant protein, an eukaryotically expressed p54 recombinant protein and an artificially synthesized polypeptide shown in Seq ID No.1. The preparation method of the monoclonal antibody for the African swine fever virus gene II type strain has the advantages that the three screening antigens are adopted for screening the hybridoma cells, and especially a special artificially synthesized polypeptide for the gene II type strain is adopted as the screening antigen, so that the prepared monoclonal antibody can guarantee the detection rate of the gene II type strain, the misdetection rate is reduced, and the quarantine working quality and efficiency are improved.
Owner:SHENZHEN AUDAQUE DATA TECH

Agricultural sunlight greenhouse

The invention relates to an agricultural sunlight greenhouse. The agricultural sunlight greenhouse comprises an outer shed formed by at least three front arch tubes identical in shape, rear arch tubes, center pillars, a top pull rod, transverse pull rods and an agricultural film, wherein each rear arch tube is of a structure which is similar to an L shape rotated clockwise for 180 degrees and is formed by an inclined top and a column, and the top pull rod is located on a junction of the front arch tubes and the ends of the inclined tops of the rear arch tubes. An inner shed is further arranged in the outer shed, support points of the center pillars are located on the junctions of front arch tubes of the inner shed and the inclined tops of the rear arch tubes, the front arch tubes of the inner shed are positioned through the transverse pull rods, inner gables are formed by gable columns and the transverse pull rods at two ends of the inner shed, the agricultural film is covered on the front arch tubes of the outer shed and outer gables, an agricultural film and a thermal insulation quilt are covered on the front arch tubes of the inner shed, rolling and unrolling of the thermal insulation quilt and the agricultural film are respectively independently controlled through a roller blind mechanism and a film rolling mechanism, and the agricultural films and thermal insulation plates are covered on the inner gables and the rear arch tubes. Buffer rooms are arranged between the inner gables and the outer gables of the same end, and the inner gables and the outer gables are respectively provided with inner shed doors and outer shed doors.
Owner:JIANGSU ACAD OF AGRI SCI +1

Degenerate reverse transcription-polymerase chain reaction (RT-PCR) detection reagent and kit for hantavirus group

The invention discloses a degenerate reverse transcription-polymerase chain reaction (RT-PCR) detection reagent and a kit for hantavirus group. The detection reagent comprises a pair of degenerate heterozygous oligonucleotide primers, and the sequences of the degenerate heterozygous oligonucleotide primers are respectively G1: GCAACAGCAACATGGTTTcartaytayac and G2: CTTCTTCATTCATATTTCCATGCarnccyttytc; the non-merger consensus sequence of the 5' ends in the primers plays a role in stabilizing the combination of a 3' merger core area and a template under the condition that the degeneracy of the primers is not increased, so that the specificity of the degenerate PCR reaction is improved, various viruses in hantavirus can be amplified and detected, the homologous unknown viruses of the extendedgenes can also be detected and the amplified target fragments can be sequenced by the detection reagent; and the kit are relatively high in sensitivity and good in hantavirus group specificity, can be used for detecting domestic popular Hantaan and Seoul viruses, and can also be used for detecting other oversea epidemic strains. The degenerate RT-PCR detection reagent and the kit for the hantavirus group are high in sensitivity, good in hantavirus specificity and universal.
Owner:中华人民共和国大榭出入境检验检疫局

Insulated mounting method for top-surface two-end rolling prevention supports of A-type independent liquid cargo tank

The invention provides an insulated mounting method for top-surface two-end rolling prevention supports of an A-type independent liquid cargo tank. The top-surface two-end rolling prevention supports comprise rib plates, mounting panels, stow-wood grooves and stow-woods. The insulated mounting mainly comprises: using foaming materials to protect each top-surface two-end rolling prevention support, and preventing heat loss caused by heat transfer between metals. Main steps of the method comprise: surface cleaning for the liquid cargo tank and the top-surface two-end rolling prevention supports, spraying of an anticorrosive coating, spraying of the foaming material, perfusion of the foaming material through die cavities, and spraying of a protective coating. Through the steps, the problem of inconvenience for insulated mounting of the top-surface two-end rolling prevention supports can be effectively solved, and because the method is directed at a structure characteristic of the A-type independent liquid cargo tank and spraying of the foaming material and perfusion of the foaming material are adopted at the same time, the insulated effect can be effectively ensured, the tightness of insulated mounting can be guaranteed, and heat outside the liquid cargo tank is prevented from transferred into the liquid cargo tank.
Owner:JIANGNAN SHIPYARD GRP CO LTD

Infectivity scrapie resistance gene rapid typing detection reagent, preparation method and application thereof

The invention discloses an infectivity scrapie resistance gene rapid typing detection reagent, and a preparation method and application thereof, which is characterized in that three sets of idiosyncratic primers and Taqman probes are designed and synthesized; and 136-position, 154-position, 171-position codons of sheep prion protein encoding gene PRNP which respectively determine scrapie resistance / sensibility are detected for judging whether sheep to be detected contain infectivity scrapie resistance gene. The invention establishes a rapid, simple and convenient gene typing method with strongspecificity and high sensitivity with the help of a real-time fluorescence quantitative PCR detection system and a subsequent end-point reading plate model; the detection time is only a plurality ofhours; and with the help of the method, a whole set of thorough early warning and monitoring system is established for preventing scrapie, can be applied to a conventional laboratory diagnosis method,is used as a test item for introducing live sheep by entry-exit inspection and quarantine departments so as to put an end to the introduction of the scrapie sensitivity varies.
Owner:ANIMAL & PLANT & FOOD INSPECTION CENT OF TIANJIN ENTRY EXIT INSPECTION & QUARANTINE BUREAU

Inserting and pulling type low-temperature container

The invention discloses an inserting and pulling type low-temperature container. The inserting and pulling type low-temperature container comprises a refrigerator and a low-temperature container body.A first-stage cold cavity is placed in a first-stage cold screen of the low-temperature container body, an elastic round sleeve and a first-stage corrugated pipe are arranged on the top walls of thelow-temperature container body and the first-stage cold cavity correspondingly, the middle of the elastic round sleeve is fixedly provided with a first lower conduction block connected with the top wall of the first-stage cold screen, a second upper conduction block and a second lower conduction block are arranged on the bottoms of the elastic round sleeve and the first-stage corrugated pipe correspondingly in a seal manner, a first upper conduction block is arranged on a first-stage cold head of the refrigerator in a sleeving manner, a second-stage cold head of the refrigerator is used for driving the second upper conduction block and the second lower conduction block to be tightly attached, the refrigerator is mounted on the top wall of the low-temperature container body through an elastic supporting device, and the upper end of the elastic round sleeve is fixed to the elastic supporting device. When the elastic supporting device is loosened, the elastic supporting device is matchedwith the elastic round sleeve to enable the refrigerator to pop out of the low-temperature container body, and complete separation of the refrigerator and the low-temperature container body is achieved.
Owner:SOUTHWEST JIAOTONG UNIV

Overvoltage protection device of chopper, chopper and electric locomotive

The invention provides an overvoltage protection device of a chopper, the chopper and an electric locomotive. The overvoltage protection device comprises a voltage detection unit, a first voltage comparison unit, a first time acquisition unit, an electric energy absorption unit and a fuse, wherein the voltage detection unit is arranged in an output end circuit of a voltage-decreasing chopper, the first voltage comparison unit is connected with the voltage detecting unit, the first time acquisition unit is connected with the first voltage comparison unit, the electric energy absorption unit is arranged behind an output end voltage detection unit of the voltage-decreasing chopper and is connected with the first time acquisition unit, and the fuse is connected in series with an input end circuit of the chopper. With the overvoltage protection device adopted, the output overvoltage of the chopper can be prevented from damaging components connected in later-stage circuits of the chopper, and therefore, the switching frequency of the chopper can be reduced under the premise that corresponding equipment is protected, and thus, the service life of the chopper can be prolonged, and the operational stability of a system using the chopper can be improved.
Owner:DATONG ELECTRIC LOCOMOTIVE OF NCR

Integrated replaceable hydrogen storage tank special for hydrogen energy vehicle

The invention discloses an integrated replaceable hydrogen storage tank special for a hydrogen energy vehicle, and relates to the technical field of soil restoration and irrigating. The integrated replaceable hydrogen storage tank includes an exhausting outlet and a set connector, a rubber ring is mounted on the outer side of the exhausting outlet, a clamping buckle is arranged on the inner side of the exhausting outlet, a shell is arranged on one side of the exhausting outlet and is further includes a steel body layer, a thermal insulating layer and an anti-corrosion layer, and the shell is connected to the outer side of the steel body layer. According to the integrated replaceable hydrogen storage tank special for the hydrogen energy vehicle, a fixing block, a clamping block, a spring, atop plate, the set connector and a hydrogen tank are arranged, the clamping block is fixed by users through the fixing block, elasticity of the spring reduces damage to internal parts of the device caused by external vibration transmitted into the device during impacting, the service life of the device is enhanced, the hydrogen tank can be inflated and exhausted by the users through the set connector, using is convenient for the users, the hydrogen tanks with the different sizes are designed according to the different driving range to meet air consumption of different vehicle models, and several capacity specifications can be designed.
Owner:WUHAN GROVE HYDROGEN AUTOMOBILE CO LTD

Insulated mounting method for bottom-surface middle rolling prevention supports of A-type independent liquid cargo tank

The invention provides an insulated mounting method for bottom-surface middle rolling prevention supports of an A-type independent liquid cargo tank. The bottom-surface middle rolling prevention supports comprise rib plates, mounting panels, stow-wood grooves and stow-woods. The insulated mounting mainly comprises: using foaming materials to protect each bottom-surface middle rolling prevention support, and preventing heat loss caused by heat transfer between metals. Main steps of the method comprise: surface cleaning for the liquid cargo tank and the bottom-surface middle rolling prevention supports, spraying of an anticorrosive coating, spraying of the foaming material, perfusion of the foaming material through die cavities, and spraying of a protective coating. Through the steps, the problem of inconvenience for insulated mounting of the bottom-surface middle rolling prevention supports can be effectively solved, and because the method is directed at a structure characteristic of the A-type independent liquid cargo tank and spraying of the foaming material and perfusion of the foaming material are adopted at the same time, the insulated effect can be effectively ensured, the tightness of insulated mounting can be guaranteed, and heat outside the liquid cargo tank is prevented from transferred into the liquid cargo tank.
Owner:JIANGNAN SHIPYARD GRP CO LTD

Cast-in-situ steel mesh frame sandwich heat-insulating shear wall and construction method thereof

ActiveCN107165310AEfficient deliveryMeet temperature deformation requirementsWallsFloorsRebarUpper floor
The invention relates to a cast-in-situ steel mesh frame sandwich heat-insulating shear wall and a construction method thereof. The abdominal wire of a steel mesh frame sandwich heat-insulating plate used by the shear wall is a straight wire, a horizontal elastic support is arranged every other floor, each horizontal elastic support comprises a plurality of vertical additional bars which are arranged horizontally at intervals and a plurality of U-shaped anchor bars which are arranged horizontally, the U-shaped anchor bars are all inserted on a heat-insulating cushion block, and are divided into two layers which are vertical align, and each layer is horizontally arranged at intervals. the heat-insulating cushion block extends and is placed between the upper and the lower steel mesh frame sandwich heat-insulating plates, the arm part and the U shape bottom part of each U-shaped anchor bar are solidified in an inner and outer wythes correspondingly, and the upper and the lower ends of each additional bar are solidified in outer wythes of an upper floor and a lower floor. In the construction process of the horizontal elastic support, the heat-insulating cushion block and the U-shaped anchor rods are mounted firstly, and then the steel mesh frame sandwich heat-insulating plates are mounted in an aligned mode to make an outer side steel mesh be connected with the upper portion of the vertical additional bar embedded in the lower floor. The cast-in-situ steel mesh frame sandwich heat insulating shear wall can solve the problems of cracking and separation of outer wythes, and is particularly suitable for high buildings.
Owner:ARCHITECTURAL DESIGN & RES INST OF TSINGHUA UNIV +1

Method for detecting multiple trace targets based on long probe

The invention discloses a method for detecting multiple targets based on a long probe. The method is characterized by comprising the steps of: 1) obtaining targets to be detected and the nucleotide sequences of internal references 18S rRNA (Ribosomal Ribose Nucleic Acid) thereof, and designing identification probes for two oligonucleotides closely connected together in the conserved region of each target to be detected and the internal reference 18S rRNA thereof; 2) designing universal primers and selecting a filler long sequence, connecting the end 3' of the universal sequence Tag2 with the end 5' of the upstream identification probe to form an upstream LDR (Ligase Detection Reaction) probe, and connecting the filler long sequence with the end 5' of the universal sequence Tag1 and the end 3' of the downstream identification probe to form a downstream LDR long probe; 3) carrying out the ligase detection reaction between the upstream LDR probe and the downstream LDR long probe, and a target sample to be detected; 4) amplifying the connected product by using the upstream and downstream universal primers, obtaining stripe information through agarose nucleic acid electrophoresis, and thus obtaining the infection condition of the mixed targets.
Owner:ZHEJIANG FORESTRY UNIVERSITY

Alkali-resistant glass fiber mesh cloth

The invention discloses alkali-resistant glass fiber mesh cloth. The mesh cloth is formed by weaving longitude lines and latitude lines, wherein all the longitude lines are arranged longitudinally atequal distance, all the latitude lines are arranged horizontally at equal distance, and each of the longitude lines is woven with all the latitude lines; all the longitude lines and all the latitude lines are glass fiber yarns, each of the glass fiber yarns comprises a plurality of monofilaments, and the diameter of each of the monofilaments is 7-10 [mu]m; the thickness of the alkali-resistant glass fiber mesh cloth is 0.2-0.7 mm; and the outer surface of the alkali-resistant glass fiber mesh cloth is coated with a binding agent layer, and the outer surface of the binding agent layer is coatedwith an alkali-resistant layer. The alkali-resistant glass fiber mesh cloth is strong in high temperature resistance, high in tensile strength and low in thermal conductivity coefficient; in addition, when the alkali-resistant glass fiber mesh cloth is compounded with resin to form an anti-ablation material, the anti-ablation property and the mechanical washing resistance of the anti-ablation material can be effectively improved, and high temperature on the surface of the anti-ablation material can be prevented from transmitting into the anti-ablation material.
Owner:合肥神舟建筑集团有限公司

Primary air system pipeline device convenient to mount

The invention discloses a primary air system pipeline device convenient to mount. The device comprises an exhaust assembly and an air inlet assembly; the exhaust assembly comprises an exhaust pipeline, a first-stage exhaust fan, a noise reduction air guide cylinder A and an exhaust rough obstruction net; the air inlet assembly comprises an air inlet pipeline, a first-stage exhaust fan, a noise reduction air guide cylinder B and an air inlet fine obstruction net; according to the beneficial effects, a passing opening is formed in the building wall, the first-stage exhaust fan, the noise reduction air guide cylinder A and the exhaust rough obstruction net are assembled to the exhaust pipeline, the first-stage exhaust fan, the noise reduction air guide cylinder B and the air inlet fine obstruction net are assembled to the air inlet pipeline, the exhaust pipeline is inserted into the building wall passing opening, the air inlet pipeline is inserted into the building wall passing opening, the device is rapid and convenient to mount, the noise reduction air guide cylinder A and the noise reduction air guide cylinder B have a certain rain sheltering effect, the noise reduction effect andthe air guide effect, flying birds can be effectively prevented from inhabiting, objects like fallen leaves outside the building wall can be effectively obstructed from floating in, and the device ishigh in practicability and good in use effect.
Owner:珠海华盛辉新风净化设备有限公司
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