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Sub-terahertz nano biosensor for detecting single or few cells

A biosensor, a small number of cell technology, applied in the direction of measuring devices, scientific instruments, individual particle analysis, etc., can solve the problems of inability to identify the signal-to-noise ratio of single cells, the inability to detect single or a small number of cells with terahertz light sources, etc., to reduce The effect of original sample volume, improvement of detection efficiency and quality, and elimination of bacterial culture

Active Publication Date: 2015-04-22
THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] In view of this, the purpose of the present invention is to provide a sub-terahertz nano-biosensor for single or a small number of cell detection, to solve the problems in the prior art that single or a small number of cells cannot be detected by a terahertz light source and single cells cannot be identified. For the problem of signal-to-noise ratio, the following technical solutions are provided:

Method used

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  • Sub-terahertz nano biosensor for detecting single or few cells
  • Sub-terahertz nano biosensor for detecting single or few cells
  • Sub-terahertz nano biosensor for detecting single or few cells

Examples

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Embodiment 1

[0029] Such as Figure 1 to Figure 3 As shown, this embodiment includes a microfluidic unit, a slot nano-antenna 4, and the microfluidic unit includes at least one microfluidic body 1 in a tubular structure, a positive pressure generating device 2 arranged at the cell inlet end of the microfluidic body 1, a set The negative pressure generating device 3 at the cell outlet of the microfluidic body, the positive pressure generating device generates positive pressure, and the negative pressure generating device generates negative pressure. Under the combined action of positive and negative pressure, the cells of the cell solution are pushed into the microfluidic body 1, To realize detection, the slot nano-antenna 4 is provided with a long and narrow slot 5 that runs through the antenna body, and the microfluidic body 1 passes through the long and narrow slot 5 one by one. enlarge.

[0030] In this embodiment, by setting the microfluidic unit and using the positive pressure and ne...

Embodiment 2

[0038] Such as Figure 4 As shown, the difference between embodiment 2 and embodiment 1 is that the microfluidic body 1 is set as a Y-shaped structure, that is, the microfluidic body is combined into a unified channel before the negative pressure generating device 3 is connected, so that multiple microfluidic bodies 1 The centralized processing of the pipes forms a negative pressure structure, and the cell solution is more likely to enter the microfluidic body 1, and the effect is better. Other structures are identical with embodiment 1, different Ao states here.

Embodiment 3

[0040] Such as Figure 5 For Example 3, the difference from Example 1 is that the microfluidic body 1 is set in a U-shaped structure, which can increase the length of the microfluidic body, and it is easier to disperse a certain number of cells to be tested, which is conducive to the entry of the cell solution Microfluidic body 1, the effect is better. Other structures are the same as in Embodiment 1, and will not be described here again.

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Abstract

The invention discloses a sub-terahertz nano biosensor for detecting single or few cells, belonging to the technical field of sensors. The sub-terahertz nano biosensor comprises a terahertz sample detection cell which consists of a microfluid unit and slot nano antennas, wherein the microfluid unit comprises at least one microflow body, a positive pressure generating unit and a negative pressure generating unit; the microflow bodies are of a tubular structure, the positive pressure generating unit is arranged at cell inlet ends of the microflow bodies, and the negative pressure generating unit is arranged at cell outlet ends of the microflow bodies; the slot nano antennas are provided with slots penetrating through antenna bodies; the microflow bodies penetrate through the slots in a one-to-one correspondence manner. By virtue of the terahertz sample detection cell consisting of the microfluid unit and the slot nano antennas, only single or few to-be-detected cells can pass through, the signal of the single cell is amplified and captured, and the cells of different characters are distinguished and identified, so that bacteria of different characters can be really, qualitatively and quantitatively detected by directly utilizing a sub-terahertz sensing technique.

Description

technical field [0001] The invention belongs to the technical field of sensors, and in particular relates to a sub-terahertz nanobiological sensor for single or small amount of cell detection. Background technique [0002] At present, the bacterial culture and identification established by Pasteur and Koch in the 19th century is still the mainstream technology and method of clinical microbiology testing. Due to the long period of bacterial culture, bacterial species identification and drug susceptibility testing often take about a week. In addition, there are many problems such as differences in culture substrates, too long culture time (for example, Mycobacterium tuberculosis takes nearly one month for culture), and difficulty in culturing and isolation (for example, Haemophilus influenzae). In the past 20 years, in order to overcome the above drawbacks, a series of molecular biology techniques and methods have emerged, mainly including real-time fluorescent PCR, digital P...

Claims

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Application Information

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IPC IPC(8): G01N15/10
Inventor 黄庆府伟灵杨翔李永川
Owner THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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