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Polydopamine quantum dot-based fluorescence enhanced detection method of sulfur dioxide derivatives

A polydopamine quantum and fluorescence-enhanced technology, applied in the field of biosensing, can solve the problems of high cytotoxicity, complex synthesis process, and less SO2 derivatives, and achieve good sensitivity and simple synthesis process

Active Publication Date: 2018-09-28
NANJING UNIV OF TECH
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Problems solved by technology

The above detection methods generally have the disadvantages of low detection limit, high cytotoxicity, unfriendly solvent and complicated synthesis process.
At present, the detection of SO in the environment and cells based on quantum dots (QDs) 2 There are few reports on derivatives

Method used

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  • Polydopamine quantum dot-based fluorescence enhanced detection method of sulfur dioxide derivatives
  • Polydopamine quantum dot-based fluorescence enhanced detection method of sulfur dioxide derivatives
  • Polydopamine quantum dot-based fluorescence enhanced detection method of sulfur dioxide derivatives

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Effect test

Embodiment 1

[0048] Embodiment 1: At room temperature, take 0.9482g of dopamine hydrochloride and deionized water to configure 10mL mixed solution in a 20mL glass bottle, make the concentration of dopamine hydrochloride in the mixed solution be 0.5M and place it in a dark room for 1 month, the color of the solution changes from colorless into a tan color, indicating the formation of polydopamine quantum dots. The prepared polydopamine quantum dots are measured by UV-Vis spectrophotometer and fluorescence spectrophotometer to draw absorption spectrum curve and fluorescence spectrum curve, such as figure 1 and figure 2 .

Embodiment 2

[0049] Embodiment 2: At room temperature, take 0.9482g of dopamine hydrochloride and deionized water to configure 10mL mixed solution in a 20mL glass bottle, make the concentration of dopamine hydrochloride in the mixed solution be 0.5M and place it in a dark room for 3 months, the color of the solution changes from colorless into a tan color, indicating the formation of polydopamine quantum dots. The prepared polydopamine quantum dots are measured by UV-Vis spectrophotometer and fluorescence spectrophotometer to draw absorption spectrum curve and fluorescence spectrum curve, such as image 3 and Figure 4 .

Embodiment 3

[0050] Embodiment 3: At room temperature, take 0.9482g of dopamine hydrochloride and deionized water to configure 10mL mixed solution in a 20mL glass bottle, make the concentration of dopamine hydrochloride in the mixed solution be 0.5M and place it in a dark room for 6 months, the color of the solution changes from colorless into a tan color, indicating the formation of polydopamine quantum dots. The prepared polydopamine quantum dots are measured by UV-Vis spectrophotometer and fluorescence spectrophotometer to draw absorption spectrum curve and fluorescence spectrum curve, such as Figure 5 and Image 6 .

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Abstract

The invention relates to a polydopamine quantum dot-based fluorescence enhanced detection method of sulfur dioxide derivatives and belongs to the technical field of nano-biosensors. The method comprises synthesizing polydopamine quantum dots (PDADs) emitting blue fluorescence at the room temperature through dopamine (DA) as a sole precursor, and adding sulfur dioxide derivatives into a buffer solution containing PDADs so that PDADs fluorescence is enhanced and the high sensitivity and selective detection of sulfur dioxide derivatives are realized. The fluorescent nanoprobe realizes the detection of sulfur dioxide derivatives in a complex system (comprising human serum and urine) and the fluorescence imaging of intracellular sulfur dioxide derivatives. The polydopamine quantum dot fluorescent probe has the advantages of simple synthesis, low biotoxicity, high sensitivity to sulfur dioxide derivative detection, good chemical stability and biocompatibility.

Description

technical field [0001] The invention relates to a preparation method of a method for detecting sulfur dioxide derivatives based on polydopamine quantum dots (Polydopamine quantum dots) fluorescence-enhanced detection, and belongs to the technical field of biosensing. Background technique [0002] Sulfur dioxide (SO 2 ) is an important harmful pollutant in the air, and a large number of epidemiological studies have confirmed that excessive SO in the human body 2 It will have a great toxic effect on cells and tissues, not only induce a large number of respiratory diseases in the human body, but also closely related to cardiovascular diseases, cancer and many neurological diseases, such as lung cancer, asthma attack, stroke, allergic reaction, migraine, Ischemic heart disease, myocardial ischemia and brain cancer. Sulfur dioxide easily hydrates to form SO 2 Derivatives: Sulfite ion (SO 3 2- ) and bisulfite ion (HSO 3 - ). Therefore, SO 2 The concentration of SO can be ...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6428G01N21/6458
Inventor 刘金华张承武慈乔乔邹昌鹏李林于海东
Owner NANJING UNIV OF TECH
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