Electrochemical aptamer sensor for quantitatively detecting ochratoxin A and application thereof

An aptamer sensor, ochratoxin technology, applied in the direction of material electrochemical variables, scientific instruments, instruments, etc., can solve problems such as complex situations

Inactive Publication Date: 2020-05-08
ANHUI SCI & TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the complexity of the actual sample, higher requirements are placed on the stability and sensitivity of the electrochemical detection method.

Method used

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  • Electrochemical aptamer sensor for quantitatively detecting ochratoxin A and application thereof
  • Electrochemical aptamer sensor for quantitatively detecting ochratoxin A and application thereof
  • Electrochemical aptamer sensor for quantitatively detecting ochratoxin A and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Construction of an electrochemical aptasensor for quantitative detection of ochratoxin A

[0036] In the electrochemical aptamer sensor for quantitative detection of ochratoxin A of the present invention, the counter electrode is a platinum wire electrode, the reference electrode is a saturated calomel electrode, and the working electrode is sequentially modified reduced graphene oxide (RGO) on the surface of the gold sheet electrode. , molybdenum disulfide (MoS 2 ) and gold nanoparticles (AuNPs), capture probe 1, capture probe 2 and ochratoxin A aptamer. The construction method of the working electrode is described in detail below.

[0037] 1. Three-dimensional ordered porous RGO-MoS 2 -Construction of AuNPs electrode

[0038] Before use, the gold sheet was ultrasonically cleaned with acetone, ethanol, and pure water for 10 min, and then dried with nitrogen to obtain a clean gold electrode for future use.

[0039] Construction of 3DOM RGO-MoS 2 - The spe...

Embodiment 2

[0051] Example 2 Using an electrochemical aptasensor to detect OTA

[0052] The electrochemical aptasensor prepared in Example 1 was used to detect different concentrations of OTA solutions (1fg / mL-0.1μg / mL) to investigate the linear detection range and detection limit of the electrochemical aptasensor for OTA.

[0053] Electrolyte: 0.01M, pH 7.4 PBS buffer solution

[0054] Add the OTA solution with a concentration in the range of 1fg / mL-0.1μg / mL dropwise to the surface of the working electrode, react at 37°C for 40min, wash the surface of the working electrode with PBS, and the S1 / AuNPs-Fe obtained in Example 1 3 o 4 @C and S2 / AuNPs-Fe 3 o 4@C each took 10 μL dropwise onto the electrode surface, incubated at 37°C for 2 h, and then washed the electrode surface with PBS. After the above treatment, the working electrode, platinum wire electrode (counter electrode) and saturated calomel electrode (reference electrode) were placed in the electrolyte, and differential pulse vo...

Embodiment 3

[0055] The specificity of embodiment 3 electrochemical aptasensors

[0056] OTA, aflatoxin B1 (AFB1), aflatoxin B2 (AFB2), aflatoxin M1 (AFM1) and zearalenone (ZEN) solutions with a concentration of 0.01ng / mL were prepared using Example 1 respectively The electrochemical aptasensor was tested to investigate the specificity of the electrochemical aptasensor prepared in Example 1.

[0057] Electrolyte is with embodiment 2. Add the mycotoxin dropwise to the surface of the working electrode in the electrochemical aptamer sensor prepared in Example 1, react at 37°C for 40min, wash the surface of the working electrode with PBS, and the S1 / AuNPs-Fe obtained in Example 1 3 o 4 @C and S2 / AuNPs-Fe 3 o 4 @C each took 10 μL dropwise onto the electrode surface, incubated at 37°C for 2 h, and then washed the electrode surface with PBS. After the above treatment, the working electrode, platinum wire electrode (counter electrode) and saturated calomel electrode (reference electrode) were...

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Abstract

The invention provides an electrochemical aptamer sensor for quantitatively detecting ochratoxin A and application thereof, and relates to the field of nano biosensors and electrochemical detection. The electrochemical aptamer sensor is a three-electrode system sensor, the working electrode is obtained by sequentially modifying the surface of a gold sheet electrode with a composite film of reducedgraphene oxide, molybdenum disulfide and nanogold, a capture probe 1, a capture probe 2 and an ochratoxin A aptamer, and every two of the capture probe 1, the capture probe 2 and the ochratoxin A aptamer are complementarily paired to form a Y-shaped structure. The electrochemical aptamer sensor disclosed by the invention is used for quantitatively detecting ochratoxin A and has the advantages ofsimplicity in operation, good stability, high sensitivity, strong specificity, good reproducibility and the like.

Description

technical field [0001] The invention relates to the fields of nano-biosensors and electrochemical detection, in particular to an electrochemical aptamer sensor for quantitative detection of ochratoxin A and its application. [0002] technical background [0003] Ochratoxins are toxic secondary metabolites produced by fungi of the genus Ochraus and Penicillium, which include seven structurally similar compounds. Among them, the most toxic and most closely related to human health belongs to ochratoxin A. Ochratoxin A is often detected in food, including wheat, corn, coffee, spices, beer, grapes and animal meat products, which will pose a great threat to human health. Because ochratoxin A cannot be completely removed in food, in order to reduce the adverse effects of ochratoxin A on human health, countries around the world have set strict limits on the content of ochratoxin A detected in food. The European Union's limit standards for ochratoxin A in unprocessed grains, dried f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/30G01N27/48
CPCG01N27/3277G01N27/3278G01N27/308G01N27/48
Inventor 彭钢杜传来张献领丁志刚仇丽妹魏欣雨赵峰
Owner ANHUI SCI & TECH UNIV
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