High sensitivity nano biosensor production method

A biosensor and high-sensitivity technology, which is applied in the field of high-sensitivity nano-biosensor production, can solve the problems of low detection sensitivity and achieve the effect of improving detection sensitivity and enhancing strength
CN101144809AInactive Publication Date: 2008-03-19INST OF OPTICS & ELECTRONICS - CHINESE ACAD OF SCI
0 Cites 23 Cited by

Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
INST OF OPTICS & ELECTRONICS - CHINESE ACAD OF SCI
Publication Date
2008-03-19
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
Patent Text Reader

Abstract

The present invention provides a producing method of the nanometer biosensor with high sensitivity. The procedures are as follows: firstly, selecting the fundus material and completing the producing of the metal nanometer array; secondly, soaking the fundus in the prepared biology activated chemical reagent solution to ensure the metal surface possess an activated group corresponding to the biology molecule, and to ensure the combination of the antigen and the silver granules array to be more easy, the biology activated chemical reagent is the octane mercaptan 1-OT and the eleven alcoholic acid 11-MUA with the hydrogen thiocyanato, the solvent is ethanol; thirdly, taking out the soaked substrate, cleaning off the leftover on the surface of the substrate and blow the substrate dry; fourthly, selecting the antigen corresponding to the detected molecule and the zero-distance coupling reagent; fifthly, mixing the antigen solution and the zero-distance coupling reagent solution, and then drip the mixed solution on the surface of the substrate for coupling reaction, and the reaction period is more than 3 hours; sixthly, rinsing the substrate and removing the leftover on the surface of the substrate and blow the substrate dry, and then the producing of the nanometer sensor is completed. The present invention improves greatly the sensitivity of the sensor.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to a method for manufacturing biomolecules of an LSPR nanometer sensor, in particular to a method for manufacturing a high-sensitivity nanometer biosensor. Background technique

[0002] LSPR (localized surface plasmon resonance) biosensor is favored by researchers for its convenience, high sensitivity, wide range of applications, real-time monitoring, etc., and is at the forefront of sensor research. It is considered the most It is a kind of potential biosensor, and the use of this new research method is of great significance to the basic research of life science, medical diagnosis and treatment.

[0003] For the research on LSPR biosensors, Northwestern University in the United States has done a lot of representative work. It has done a considerable part of the preliminary experimental research work on the use of nanoparticles and structures for biomolecular markers and detection, using the truncated hexagonal distribution The t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More