Preparation method and application of two-dimensional nano rare earth borate laccase sensor

A two-dimensional nano, borate technology, applied in instruments, scientific instruments, measuring devices, etc., can solve problems such as desorption activity, loss, laccase leakage, etc., and achieve simple methods, easy large-scale production, and high sensitivity. Effect

Inactive Publication Date: 2014-01-08
TONGJI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in the process of laccase immobilization, due to the laccase itself, carrier, reaction reagent, process conditions and other related factors, there are problems such as laccase leakage, desorption and activity loss.

Method used

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  • Preparation method and application of two-dimensional nano rare earth borate laccase sensor
  • Preparation method and application of two-dimensional nano rare earth borate laccase sensor
  • Preparation method and application of two-dimensional nano rare earth borate laccase sensor

Examples

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Embodiment 1

[0029] first, Rare earth borate SmBO 3 Preparation of nanosheets:

[0030] In this experiment, a 20 mL sealed hydrothermal reaction kettle lined with polytetrafluoroethylene was used to prepare samarium borate micro-nano materials by solid-state hydrothermal method under medium temperature conditions. The detailed reaction process is described as follows: Add 209.2 mg Sm sequentially to a clean and dry PTFE liner 2 o 3 (0.6 mmol), 44.5 mg H 3 BO 3 (0.72 mmol, 10% excess) and 14 ml H 2O; then put the liner into an ultrasonic cleaner to disperse for 15 min; take out and wipe off the moisture outside the liner, put it into a reaction kettle and seal it; put the closed reaction kettle into a temperature-controlled constant temperature drying oven for 200 ℃ and kept for 36 hours; after the reaction was completed, it was naturally cooled to room temperature, and then separated and washed with a centrifugal separator. After washing 4 times with deionized water and 4 times w...

Embodiment 2

[0038] first, Rare earth borate GdBO 3 Preparation of nanosheets:

[0039] In this experiment, a 20 mL sealed hydrothermal reaction kettle lined with polytetrafluoroethylene was used to prepare samarium borate micro-nano materials by solid-state hydrothermal method under medium temperature conditions. The detailed reaction process is described as follows: 217.5 mg Gd was sequentially added to a clean and dry PTFE liner 2 o 3 (0.6 mmol), 44.5 mg H 3 BO 3 (0.72 mmol, 10% excess) and 14 ml H 2 O; then put the liner into an ultrasonic cleaner to disperse for 15 min; take out and wipe off the moisture outside the liner, put it into a reaction kettle and seal it; put the closed reaction kettle into a temperature-controlled constant temperature drying oven for 200 ℃ and kept for 36 hours; after the reaction was completed, it was naturally cooled to room temperature, and then separated and washed with a centrifugal separator. After washing 4 times with deionized water and 4 ...

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Abstract

The invention belongs to the technical field of preparation and application of nano/micrometer scale materials, and particularly relates to a preparation method and application of a two-dimensional nano rare earth borate laccase sensor. The preparation method specifically comprises the steps of firstly, synthesizing a rare earth borate nano material by a solid-state hydrothermal process; and then immobilizing laccase in a lamellar structure of the rare earth borate nano material to prepare the two-dimensional nano rare earth borate laccase sensor. The electrochemical property of the two-dimensional nano rare earth borate laccase sensor in a phenol solution is researched, and the two-dimensional nano rare earth borate laccase sensor is applied to monitoring and processing of phenols pollutants in a water body. The laccase is immobilized by the laminar borate nano material, and the preparation method is simple and efficient; a method for determining hydroquinone and pyrocatechol by the laccase/rare earth borate nano biosensor is high in sensitivity, simple and easy to operate, and high in application potential.

Description

technical field [0001] The invention relates to a preparation method of a two-dimensional nanometer rare earth borate laccase sensor. It belongs to the technical field of nano / microscale material preparation and application. Background technique [0002] In recent decades, the research on the preparation and properties of rare earth borates has received extensive attention. The reasons for this phenomenon are mainly attributed to: 1) its interesting microscopic morphology; 2) its special properties. It is well known that the diversification of the microscopic morphology of substances is not only related to different preparation routes, but also related to the diversity of crystal structures. The main reason for the diversity of rare earth borate crystal structures is the variability of the structural units and spatial positions of borate anions. The general chemical formula of rare earth borate is LnBO 3 , where B atoms can coordinate with three O atoms to form planar and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
Inventor 胡萍周心琳吴庆生
Owner TONGJI UNIV
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