Sub-THz nano-biosensor for quickly frame-detecting bacteria and detection method thereof

A biosensor and sub-terahertz technology, applied in the field of sensors, can solve problems such as the inability to identify single-cell spectral signals and the inability to detect single-cell terahertz light sources, so as to solve the problems of sensitivity and specificity, reduce the volume of original sample liquid, Effects without marking

Active Publication Date: 2015-04-08
THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] In view of this, the purpose of the present invention is to provide a sub-terahertz nano-biosensor and its detection method for rapid frame detection of bacteria, so as to solve the problem that single cells cannot be detected by a terahertz light source and single cells cannot be identified in the prior art. For the problem of spectral signals, the following technical solutions are provided:

Method used

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  • Sub-THz nano-biosensor for quickly frame-detecting bacteria and detection method thereof
  • Sub-THz nano-biosensor for quickly frame-detecting bacteria and detection method thereof
  • Sub-THz nano-biosensor for quickly frame-detecting bacteria and detection method thereof

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Embodiment 1

[0042] Such as Figure 1 to Figure 3 As shown, one of the sub-terahertz nano-biosensors used for rapid frame detection of bacteria in the present invention includes a microfluidic unit and a slot nano-antenna 4, and the microfluidic unit includes at least one microfluidic body 1 in a tubular structure, arranged on The positive pressure generating device 2 at the cell inlet end of the microfluidic body 1, and the negative pressure generating device 3 arranged at the cell outlet end of the microfluidic body, the positive pressure generating device generates positive pressure, and the negative pressure generating device generates negative pressure. Next, the cells of the cell solution are pushed into the microfluidic body 1 to realize the detection. The slotted nano-antenna 4 is provided with a long and narrow groove 5 that runs through the antenna body, and the microfluidic body 1 passes through the long and narrow groove 5 one by one. When the cell to be tested passes through t...

Embodiment 2

[0051] Such as Figure 4 As shown, it is an embodiment 2 of the sub-terahertz nano-biosensor for rapid frame detection of bacteria, which is one of the present inventions. The difference from embodiment 1 is that the microfluidic body 1 is set as a Y-shaped structure, that is, the microfluidic body 1 is arranged in a Y-shaped structure. The negative pressure generating device 3 is merged into a unified channel before being connected, which is convenient for centralized processing of multiple microfluidic body 1 pipelines to form a negative pressure structure, and the cell solution is easier to enter the microfluidic body 1, and the effect is better. Other structures are identical with embodiment 1, different Ao states here.

Embodiment 3

[0053] Such as Figure 5 For Example 3, the difference from Example 1 is that the microfluidic body 1 is set in a U-shaped structure, which can increase the length of the microfluidic body, and it is easier to disperse a certain number of cells to be tested, which is conducive to the entry of the cell solution Microfluidic body 1, the effect is better. Other structures are the same as in Embodiment 1, and will not be described here again.

[0054] Two, the second of the present invention: a detection method using the sub-terahertz nano-biosensor for rapid frame detection of bacteria, its operation steps:

[0055] 1) Fully dilute the sample solution;

[0056] 2) pump the sample dilution prepared in step 1) into the microfluidic unit with a micropump;

[0057] 3) The positive and negative pressure generating devices in the microfluidic unit are activated, and the sample dilution is sent into the microfluidic body;

[0058] 4) Confirm whether the cells in the sample dilution ...

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Abstract

The invention discloses a sub-THz nano-biosensor for quickly frame-detecting bacteria and a detection method thereof, and belongs to the technical field of sensors. The sensor includes a microfluid unit and a sample detection pool consisting of slot nanotube antennas, wherein the microfluid unit includes at least one microfluid body which is with a tubular structure, a positive pressure generating device arranged at the cell inlet end of the microfluid body, and a negative pressure generating device arranged at the cell outlet end of the microfluid body. The invention also discloses a detection method of the sensor, wherein bacterium sample liquid is fully diluted and passes through the microfluid sample detection pool to be subjected to THz spectrum detection. According to the sub-THz nano-biosensor for quickly frame-detecting bacteria and the detection method thereof, enough negative pressure is generated in the microfluid, so that cells to be detected only can pass through the microfluid body with small diameter singly or in small number, so that detection specificity is effectively improved; in addition, the signal of single cell is amplified by the slot nanotube antennas, so that the sensitivity problem of single cell detection is thoroughly solved, and thereby the bacterium cells with different characters can be distinguished and recognized.

Description

technical field [0001] The invention belongs to the technical field of sensors, and in particular relates to a sub-terahertz nano-biological sensor for rapid frame detection of bacteria and a detection method thereof. Background technique [0002] At present, the bacterial culture and identification established by Pasteur and Koch in the 19th century is still the mainstream technology and method of clinical microbiology testing. Due to the long period of bacterial culture, bacterial species identification and drug susceptibility testing often take about a week. In addition, there are many problems such as differences in culture substrates, too long culture time (for example, Mycobacterium tuberculosis takes nearly one month for culture), and difficulty in culturing and isolation (for example, Haemophilus influenzae). In the past 20 years, in order to overcome the above drawbacks, a series of molecular biology techniques and methods have emerged, mainly including real-time f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/3586
Inventor 黄庆府伟灵杨翔李永川
Owner THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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