Composition for inhibiting streptococcus mutans and application of composition

A technology of Streptococcus mutans and a composition, which is applied in the directions of drug combinations, antibacterial drugs, medical preparations containing active ingredients, etc., can solve the problems of limited inhibitory effect, etc. The effect of preventing dental caries

Active Publication Date: 2020-08-28
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Biofilm bacteria are highly resistant to antibiotics and host immune defense mechanisms, and some existing anti-caries methods, such as the conventional antibacterial drug chlorhexidine, have very limited inhibitory effects on oral biofilms

Method used

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  • Composition for inhibiting streptococcus mutans and application of composition
  • Composition for inhibiting streptococcus mutans and application of composition
  • Composition for inhibiting streptococcus mutans and application of composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1: the synthesis of ferric oxide nanozyme

[0027] The hydrothermal synthesis method prepares iron ferric oxide nanozyme, and synthetic method is as follows:

[0028] Mix 10 mL of ethylene glycol with 30 mL of polyethylene glycol, add 0.4 g of anhydrous ferric chloride, and stir with a magnetic stirrer to fully dissolve the anhydrous ferric chloride. Add 3.6g NaAC·3H 2 O continued to stir and mix, reacted the reaction solution at 200°C for 12h, washed with ethanol and water to remove impurities. The transmission electron microscope image of ferric oxide nanozyme prepared by this method is as follows: figure 1 As shown, the results indicated that spherical nanoparticles with a diameter of about 40 nm were prepared.

Embodiment 2

[0029] Embodiment 2: the influence of the combination of Lactobacillus plantarum, lactate oxidase and ferric oxide nano-enzyme on oral biofilm (43h)

[0030] 1. Cultivation method of mixed biofilm

[0031] Pick Streptococcus mutans and Lactobacillus plantarum colonies from the plate, and culture them in LMW+1% glucose medium and MRS medium, respectively, in a medium containing 5% CO 2 Activation overnight at 37°C in an incubator. Transfer the next day and culture to OD in the incubator 600 reach 1.

[0032] The saliva was collected on an empty stomach and added to the same volume of phosphate buffered saline, then PMSF (1 mM) was added to mix, and the bacteria were filtered. Soak the hydroxyapatite discs or human tooth slices used in the experiment in the obtained sterile saliva, incubate in a 37°C incubator for 1 hour, and then wash them with sterile water.

[0033] Brain Heart Infusion Broth (BHI) supplemented with 1% glucose was used as the medium for cultivating mixed ...

Embodiment 3

[0047] Embodiment 3: Under the observation of electron microscope, the influence of the combination method involved in the present invention on the production of oral biofilm (43h)

[0048] 1. The impact of the combination method involved in the present invention on the generation of oral biofilm (43h) under the biological scanning electron microscope

[0049] Four groups were set up in the experiment: 1.S.m group 2.mix group 3.mix+LOD group 4.mix+LOD+MNPs group

[0050] Streptococcus mutans and Lactobacillus plantarum were activated overnight, transferred the next day, and cultivated to OD at 37°C in a 5% carbon dioxide incubator 600 to 1. The hydroxyapatite slices were soaked in sterile saliva and transferred to a 37°C oven for 1 hour.

[0051] The total volume of each well in the 24-well plate is 2.8mL, add Streptococcus mutans and Lactobacillus plantarum bacteria solution according to the experimental settings, and control the initial number of bacteria to 10 5 CFU / mL a...

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Abstract

The invention discloses a composition for inhibiting streptococcus mutans and an application of the composition in preparation of medicines for inhibiting the streptococcus mutans. The composition comprises lactobacillus plantarum, lactate oxidase and ferroferric oxide nano-enzyme. Lactic acid continuously secreted by the lactobacillus plantarum is continuously converted into hydrogen peroxide under the linkage catalytic action of the lactate oxidase and the ferroferric oxide nano-enzyme, and hydroxyl radicals better than the hydrogen peroxide in bactericidal activity are further generated, sothat the effects of sterilization and inhibition of biofilm formation are achieved. The composition exhibits a mild and sustained bactericidal effect. The lactobacillus plantarum-lactate oxidase-ferroferric oxide nano-enzyme composition provided by the invention can generate a relatively good inhibition effect on main pathogenic bacteria, namely the streptococcus mutans, of dental caries in the oral cavity and a biofilm formed by the streptococcus mutans, and can become a new means for preventing and treating the dental caries.

Description

technical field [0001] The invention belongs to the technical field of streptococcus mutans inhibitors, in particular to a composition for inhibiting streptococcus mutans and its application. Background technique [0002] Biofilm is a bacterial aggregate membrane formed by some polysaccharide matrix secreted by bacteria to wrap the bacterial community. Many human diseases are caused by biofilm, one of which is dental caries. Studies have shown that the main pathogenic bacteria of dental caries is Streptococcus mutans (S.mutans), which can easily adhere to the surface of our teeth, use the sugars in the oral cavity to produce acid and form a dense biofilm. These biofilms tightly attached to the tooth surface are difficult to remove. It provides a barrier for the bacteria in the film, which greatly increases the difficulty of sterilization; the acidic substances produced by the bacteria will demineralize the teeth. With the loss of calcium, phosphorus and other substances, St...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/44A61P1/02A61P31/04A61K35/747A61K33/26
CPCA61K35/747A61K38/44A61K33/26A61P1/02A61P31/04A61K2300/00
Inventor 许卓斌程璐高利增王辉辉张唯王砚秋丁黎明
Owner YANGZHOU UNIV
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