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Neutral red blended silicon dioxide nano biological biological sensor and its preparing method

A biosensor and silicon dioxide technology, applied in the direction of instruments, scientific instruments, and electrochemical variables of materials, can solve the problems of high enzyme consumption, high sensor cost, poor stability, etc., and achieve improved sensitivity and stability, good biological Compatibility and biocompatibility, effect of improving stability

Inactive Publication Date: 2005-03-02
EAST CHINA NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The introduction of the second electronic mediator, carbon nanotubes, improves the response sensitivity of the sensor and overcomes the defect of poor stability caused by the loss of ferricyanide
However, since the reaction layers are sequentially stacked in the cavity with capillary pores, the amount of enzyme used is relatively high, which increases the cost of the sensor.

Method used

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  • Neutral red blended silicon dioxide nano biological biological sensor and its preparing method
  • Neutral red blended silicon dioxide nano biological biological sensor and its preparing method
  • Neutral red blended silicon dioxide nano biological biological sensor and its preparing method

Examples

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Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Preparation method of the glucose sensor of the present invention for detecting glucose content.

[0032] First use the inverse microemulsion method, mix 7.5mL cyclohexane, 1.8mL n-hexanol and 1.77mL polyethylene glycol octylphenyl ether (OP) and stir for 30min, the surfactant OP can be replaced by TritonX-100, etc. The molar ratio of water to surfactant (W=10) was maintained. Then slowly add 400 μL 1×10 -2 mol L -1 The neutral red aqueous solution was uniformly stirred and reacted at room temperature for 30 minutes to form a stable water-in-oil system. Then slowly add 60-100 μL tetraethyl orthosilicate (TEOS) and 30-60 μL ammonia water (NH 3 ·H 2 O), at room temperature, continue to fully stir the reaction for 24h. The molar ratio of water to TEOS (W=10) was maintained. In the process of core-shell nanoparticles synthesized by water-in-oil microemulsion, under the catalysis of ammonia water, TEOS is gradually hydrolyzed and polycondensed to form a silic...

Embodiment 2

[0039] Example 2 The preparation method of the sensor for detecting lactic acid.

[0040] In the preparation of NR-SiO 2 For nanoparticles, change 1×10 -2 mol L -1 The amount of the neutral red aqueous solution is 300 μ L, and other preparation and treatment process are the same as embodiment 1. Characterized by TEM, the NR-SiO at this time 2 The particle size is 20±4nm (see Figure 5 ).

[0041] Bioactive enzymes and BSA and glutaraldehyde cross-linking method, except that 10 mg BSA and 0.4 mg LOD were dissolved in 100 μL of 0.1 mol L -1 (pH 6.9) in the phosphate buffered solution that is formulated as enzyme solution is different from embodiment 1, all the other preparation steps are identical with embodiment 1. The modification process is also the same as in Example 1.

[0042] After testing, the NR-SiO 2 The current response value of the nano-modified biosensor to lactic acid was 2.3 times that of the sensor prepared without nano-modification under the same conditi...

Embodiment 3

[0043] Example 3 Preparation of L-glutamic acid sensor

[0044] In the preparation of NR-SiO 2 For nanoparticles, the stirring reaction time was changed to 12h, and other preparation and treatment processes were the same as in Example 1. Characterized by TEM, the NR-SiO at this time 2 The particle size is 40±5nm.

[0045] Bioactive enzymes and BSA and glutaraldehyde cross-linking process, except that 10mg BSA and 0.2mg L-GOD were dissolved in 100μL 0.1mol L -1 (pH 6.9) in the phosphate buffer solution, is mixed with enzyme solution, is different from embodiment 1, and all the other processes are all the same as embodiment 1. After testing, the NR-SiO 2 The current response value of the nano-modified bioarray sensor to glutamate was 8.3 times that of the sensor prepared without nano-modification under the same conditions. After testing, the linear range of the sensor is 5.0×10 -7 ~1.0×10 -2 mol L -1 , the lowest detection limit of 2.0×10 -7 mol L -1 , and the correlat...

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Abstract

The invention relates to a nanometre biosensor and its preparing method. The working electrode of the sensor consists of modifier nuclear capsid NR-SiO2 nanometer particle on the carbon electrode, dextrose oxydizing enzyme and lactate oxidase, L-glutematic oxidase, xanthic oxidase, etc. Thin layer detection cell is a hollow polytetrafluoroethylene film. The counter electrode consists of stainless steel electrode and a liquid inlet hole and outlet hole. The working electrodes, the thin layer detection cell and the counter electrode are set to a integration by screw. The preparation of the sensor is: first, modifying nuclear capsid nanometer particle onto the carbon electrode, after crosslink biological active enzyme and BSA with glutaraldehyde, modifying them on the nanometre NR-SIO2 layer, airing it dry and finish.it. The invention has the advantages of low cost, simple technique, and it can be used to detect various solutions containing dextrose with high sensitivity, stability, wide linear range and low detection line, with little demand of the sample quantity.

Description

technical field [0001] The invention relates to a novel nano-modified biosensor and a preparation method thereof, belonging to the technical fields of biosensor technology and nano-biological detection. Background technique [0002] Biosensors can quickly, accurately and conveniently measure the content of glucose and lactic acid in blood, food and fermentation industries, and have attracted the attention of people in the industry. However, currently existing biosensors generally have the defects of low sensitivity, poor anti-interference, low stability and high manufacturing cost. For example, the Chinese patent application "Blood Lactic Acid Electrochemical Sensor" (application number 03128815.4) is composed of a substrate provided with a blood sample measurement working area, a working electrode and a reference electrode sequentially covered on the substrate. The substrate (inside the blood sample measurement working area) is also covered with an...

Claims

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Application Information

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IPC IPC(8): G01N27/30G01N27/327G01N27/333
Inventor 金利通张芬芬万巧王晓丽李陈鑫朱自强鲜跃仲张文
Owner EAST CHINA NORMAL UNIV
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