Preparation method of three-dimensional graphene lactic acid sensor

Abstract

The invention relates to a preparation method of a three-dimensional graphene lactic acid sensor. The preparation method comprises the following steps: promoting growth of a three-dimensional graphene nano wall by virtue of a plasma-enhanced chemical vapor deposition method by taking a copper foil as a substrate; drifting a three-dimensional graphene block on the surface of an Fe(NO3)3 solution so as to etch the copper foil, cleaning the copper foil by virtue of ultra-pure water and transferring the copper foil to a polyethylene terephthalate (PET) base material; printing three electrodes on the surface of the three-dimensional graphene / PET by virtue of a screen printing technology, and immobilizing lactase oxidase on the surface of the three-dimensional graphene by virtue of a chitosan-glutaraldehyde cross-linking method, so as to conduct functionalizing treatment on the electrodes; and finally, conduct sensitivity, stability, real-time performance and specificity lactic acid detection on the electrodes with the assistance of an electrochemical workstation, so that the required three-dimensional graphene lactic acid sensor is obtained. According to the preparation method disclosed by the invention, the three-dimensional graphene lactic acid sensor is prepared in a mode of being economic and environment-friendly, simple and efficient and controllable in quality, and due to the graphene nano wall which has the advantages of being rich in three-dimensional conductive network structure, huge in specific surface area and the like, the sensitivity and the real-time performance of lactic acid detection can be effectively improved; and the sensor is applicable to the fields of medical care and health.

Description

technical field [0001] The invention relates to a preparation method of a three-dimensional graphene lactic acid sensor, which belongs to the technical field of biochemical sensors, in particular to a preparation method of a wearable three-dimensional graphene lactic acid sensor. Background technique [0002] Lactic acid, as a product of anaerobic glycolysis of glucose, will accumulate in large amounts when the human body is hypoxic and over-fatigued. Its content in body fluids and tissues can effectively reflect whether local tissues of the human body are hypoxic and whether they exercise excessively. It can be seen that the real-time monitoring of human lactic acid content is particularly important. However, the general lactic acid detector on the market is an invasive sensor, which collects the capillary blood of the subject’s fingertips through needle pricking. Repeated collection is not only harmful, but also cannot achieve real-time monitoring, especially for various ...

AI Technical Summary

Problems solved by technology

[0004] The application publication number is CN105116028A, which discloses a graphene-modified lactic acid biosensor and its preparation method. The lactic acid biosensor is a miniaturized lactic acid sensor that can measure the lactic acid content in human serum, but the material still belongs to The wearable lactic acid sensor based on two-dimensional carbon particles / carbon fibers still has limitations in electrical conductivity and biocompatibility, and the stretchability of the material itself is not strong

At the same time, the patent uses oxidized or carboxylated graphene. Due to its structural defects, the material itself has a low electron transmission speed, which affects the efficiency of the sensor.

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