Preparation method of lactic acid fluorescent nano biological probe based on enzyme catalytic reaction of cobalt ion coordination type boron quantum dots

A technology for catalytic reactions and biological probes, applied in nanotechnology, chemical instruments and methods, nanotechnology, etc. for materials and surface science

Active Publication Date: 2021-08-24
QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no research on the transition metal cobalt ion (Co 2+ ) doped liquid phase exfoliation to prepare cobalt-coordinated boron quantum dots (Co-BQDs) and its fluorescent nanobiological probes for the detection of lactic acid in domestic and foreign literature and patent reports

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  • Preparation method of lactic acid fluorescent nano biological probe based on enzyme catalytic reaction of cobalt ion coordination type boron quantum dots

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Embodiment 1

[0017] The preparation method and detection principle of a cobalt ion-coordinated boron quantum dot-based lactic acid fluorescent nanobiological probe based on an enzyme-catalyzed reaction are as follows: figure 1 As shown, the specific preparation steps are as follows:

[0018] Measure 25 milliliters of acetone, add 50 milligrams of boron powder, and add 1 milliliter of freshly prepared cobalt nitrate hexahydrate aqueous solution with a concentration of 0.1 mol / liter to it dropwise under stirring, and form a homogeneous mixed solution-A after thorough stirring. The mixed solution-A was placed in an ultrasonic cell pulverizer, ultrasonicated for 1 minute, followed by cooling for 10 minutes, and the ultrasonic treatment and cooling were repeated 5 times to prepare a cobalt ion-coordinated boron nanosheet Co@BNSs dispersion. Under stirring, add 1 ml of an aqueous solution dissolved with 5 mg of glutathione GSH dropwise to the above dispersion to form a homogeneous mixture-B, whi...

Embodiment 2

[0021] The preparation method and detection principle of a cobalt ion-coordinated boron quantum dot-based lactic acid fluorescent nanobiological probe based on an enzyme-catalyzed reaction are as follows: figure 1 As shown, the specific preparation steps are as follows:

[0022]Measure 35 milliliters of acetone, add 70 milligrams of boron powder, add 3 milliliters of freshly prepared cobalt nitrate hexahydrate aqueous solution with a concentration of 0.4 mol / liter to it under stirring, and form a homogeneous mixed solution-A after thorough stirring. The mixed solution-A was placed in an ultrasonic cell pulverizer, subjected to ultrasonic treatment for 3 minutes, followed by cooling for 15 minutes, and repeated operations of ultrasonic treatment and cooling for 7 times to prepare a cobalt ion-coordinated boron nanosheet Co@BNSs dispersion. Under stirring, 3 ml of an aqueous solution in which 7 mg of glutathione GSH was dissolved was added dropwise to the above dispersion to for...

Embodiment 3

[0025] The preparation method and detection principle of a cobalt ion-coordinated boron quantum dot-based lactic acid fluorescent nanobiological probe based on an enzyme-catalyzed reaction are as follows: figure 1 As shown, the specific preparation steps are as follows:

[0026] Measure 40 milliliters of acetone, add 80 milligrams of boron powder, add 5 milliliters of freshly prepared 0.8 mol / liter cobalt nitrate hexahydrate aqueous solution dropwise under stirring, and form a homogeneous mixed solution-A after thorough stirring. The mixed liquid-A was placed in an ultrasonic cell pulverizer, ultrasonicated for 5 minutes, followed by cooling for 20 minutes, and the ultrasonic treatment and cooling were repeated 10 times to prepare a cobalt ion-coordinated boron nanosheet Co@BNSs dispersion. Under stirring, add 4 ml of an aqueous solution in which 8 mg of glutathione GSH has been dissolved dropwise to the above dispersion to form a homogeneous mixture-B, which is placed in 100 ...

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Abstract

The effect of the invention is to disclose a preparation method of a lactic acid fluorescent nano biological probe based on the enzyme catalytic reaction of the cobalt ion coordination type boron quantum dots. The preparation method comprises the following steps: by taking boron powder as a precursor, in the presence of cobalt ions, preparing cobalt ion coordinated boron nanosheets by adopting an ultrasonic-assisted liquid phase stripping method, adding glutathione (GSH), and carrying out solvent heat treatment on a mixed solution, so as to prepare the novel GSH surface modified cobalt ion coordinated boron quantum dots Co-coated BQDs. In the presence of lactate oxidase LOx, lactic acid LA is catalytically oxidized by LOx enzyme to generate pyruvic acid and hydrogen peroxide H2O2, and fluorescence of Co-BQDs is quenched under the oxidation effect of H2O2. By fitting the linear relationship between the fluorescence emission peak intensity of the Co-BQDs and the LA concentration in the corresponding mixed system, the fluorescent nano biological probe based on the Co-BQDs and LOx enzyme catalytic reaction is constructed, and is used for high-specificity quantitative detection of lactic acid in biological fluid.

Description

technical field [0001] The invention belongs to the technical field of preparation of metal ion-coordinated boron quantum dots and lactic acid fluorescent nano-biological probes, and specifically relates to a method for preparing cobalt ion-coordinated boron quantum dots-based lactic acid fluorescent nano-biological probes based on enzyme-catalyzed reactions. The prepared probe can be used for highly specific quantitative detection of lactic acid in biological fluids. Background technique [0002] There are only three electrons outside the boron nucleus, presenting a serious electron deficiency state, the boron element sp 2 Valence electrons are easy to hybridize, have a large coordination number, and have a short covalent radius, making it easy to form strong and directional bonds with other elements. Theoretical calculation studies show that the structure of two-dimensional boronene is unstable when exposed to air, and boron (B) is easy to react with oxygen (O 2 ) reacts...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/02C09K11/63B82Y20/00B82Y30/00B82Y40/00G01N21/64
CPCC09K11/025C09K11/63B82Y20/00B82Y30/00B82Y40/00G01N21/6428G01N2021/6432G01N2021/6417Y02A50/30
Inventor 金辉孙泽君杨梦桂日军
Owner QINGDAO UNIV
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