Method for preparing acetyl phosphate on basis of biological enzymes

A technology of acetyl phosphate and biological enzymes, which is applied in the field of preparation of high-energy phosphoric acid compounds, can solve the problems of environmental pollution, low conversion rate of raw materials, high production cost, etc., and achieve the effects of low residual rate, high product purity and low cost

Active Publication Date: 2015-01-07
HUNAN FLAG BIOTECHNOLOGY CO LTD
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Problems solved by technology

[0004] Aiming at a series of problems such as low raw material conversion rate, high production cost, and environmental pollution in the technology of preparing acetyl phosphate by chemical method in the prior art, the purpose of the present inve...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1. Pour 0.5mol lactic acid into a glass beaker, then pour 0.5mol of commercially available 85% phosphoric acid, dilute to 1000mL with water, stir well, then add magnesium chloride and Tris-HCl reagents in order to make their final concentrations reach 10mM respectively and 110mM, adjust the pH to 5.5-6.0 with sodium hydroxide.

[0027] 2. Add 3000 U of immobilized lactate oxidase and 5000 U of immobilized pyruvate oxidase, maintain the temperature in the water bath at 25°C, and continue the reaction for 3.5 hours, during which the pH is maintained at 5.5-6.0 with 1M sodium hydroxide.

[0028] 3. Sieve filtration to separate the immobilized enzyme and the reaction solution.

[0029] 4. Put 40 g of lithium carbonate into the separated reaction solution, stir at room temperature for 1 hour to obtain a precipitate, filter and dry to obtain 75.54 g of the product dilithium acetylphosphate, with a purity of 99.8%. The conversion rate of lactic acid was 99.4%, and the convers...

Embodiment 2

[0031] 1. Pour 1.5mol lactic acid into a glass beaker, then add 1.5mol sodium phosphate, dilute to 1000mL with water, stir well, then add magnesium chloride and Tris-HCl reagents in order to make their final concentrations reach 25mM and 100mM respectively, use Sodium hydroxide is used to adjust the pH to 6.0-6.5.

[0032] 2. Add 3500 U of immobilized lactate oxidase and 7000 U of immobilized pyruvate oxidase, maintain the temperature in the water bath at 30°C, and continue the reaction for 3.0 hours, during which the pH is maintained at 6.0-6.5 with 1M sodium hydroxide.

[0033] 3. Sieve filtration to separate the immobilized enzyme and the reaction solution.

[0034] 4. Put 120 g of lithium carbonate into the separated reaction solution, stir at room temperature for 1.5 hours to obtain a precipitate, filter and dry to obtain 226.40 g of the product dilithium acetylphosphate, with a purity of 99.8%. The conversion rate of lactic acid was 99.2%, and the conversion rate of pho...

Embodiment 3

[0036] 1. Pour 0.4mol lactic acid into a glass beaker, then pour 0.4mol of commercially available 85% phosphoric acid, dilute to 1000mL with water, stir well, then add magnesium chloride and Tris-HCl reagents in order to make their final concentrations reach 5mM respectively and 95mM, adjust the pH to 4.8-5.3 with sodium hydroxide.

[0037] 2. Add 1800 U of immobilized lactate oxidase and 3500 U of immobilized pyruvate oxidase, maintain the temperature in the water bath at 20°C, and continue the reaction for 1 hour, during which time the pH is maintained at 4.8-5.3 with 1M sodium hydroxide.

[0038] 3. Sieve filtration to separate the immobilized enzyme and the reaction solution.

[0039] 4. Put 30 g of lithium carbonate into the separated reaction solution, stir at room temperature for 2 hours to obtain a precipitate, filter and dry to obtain 60.08 g of the product dilithium acetylphosphate, with a purity of 94.3%. The conversion rate of lactic acid was 95.2%, and the conver...

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Abstract

The invention discloses a method for preparing acetyl phosphate on the basis of biological enzymes. The method comprises the following steps: carrying out enzyme catalysis reaction on lactic acid and/or lactate and phosphoric acid and/or phosphate and/or biphosphate by using an immobilized oxidase lactate-immobilized pyruvate oxidase composite enzyme as a catalyst; and after the reaction finishes, filtering and precipitating to obtain the high-purity acetyl phosphate. The method is simple to operate, has the advantages of mild reaction conditions, high raw material conversion rate, low cost and environmental protection, and satisfies the industrial production.

Description

technical field [0001] The invention relates to a method for preparing acetyl phosphate based on biological enzymes, belonging to the field of preparation of high-energy phosphoric acid compounds. Background technique [0002] Acetyl phosphate is a high-energy phosphate compound. Its high-energy phosphate bond can regenerate the expensive cofactor ATP in situ under the action of enzymes, so it has a wide range of application values ​​in the biocatalysis industry. For example, in the enzymatic synthesis of glutathione and phosphocreatine, only a small amount of ATP needs to be added to the reaction system to make the enzymatic synthesis continue, avoiding the synthesis reaction to obtain products purely at the cost of consuming expensive ATP, Thereby greatly reducing the production cost of the product. [0003] At present, the methods for preparing acetyl phosphate are all chemical methods. Generally, acid anhydride and phosphoric acid or phosphate are used as raw materials...

Claims

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Application Information

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IPC IPC(8): C12P9/00
Inventor 许岗吴峰曾红宇
Owner HUNAN FLAG BIOTECHNOLOGY CO LTD
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