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Method for detecting lactic acid by visual electrochemical luminescence sensor based on bipolar electrode array-micro-fluidic chip

A microfluidic chip and bipolar electrode technology, applied in the field of biosensors, can solve the problems of limited clinical detection application, short service life, poor anti-interference, etc., and achieve the effect of promoting electron transfer, reducing detection cost, and low cost.

Inactive Publication Date: 2017-02-22
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of enzymes can ensure the selectivity of the sensor, but the immobilization method of the enzyme and its activity are still a problem that needs to be further studied
In addition, the introduction of intermediaries can greatly improve the performance of the sensor, but it also introduces complexity and limitations
At present, biosensors for the detection of lactic acid concentration in human serum have been reported, but due to the shortcomings of poor anti-interference, narrow linear range, and short service life, they are limited in clinical detection applications.

Method used

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  • Method for detecting lactic acid by visual electrochemical luminescence sensor based on bipolar electrode array-micro-fluidic chip
  • Method for detecting lactic acid by visual electrochemical luminescence sensor based on bipolar electrode array-micro-fluidic chip
  • Method for detecting lactic acid by visual electrochemical luminescence sensor based on bipolar electrode array-micro-fluidic chip

Examples

Experimental program
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Effect test

Embodiment 1

[0054] Example 1 Preparation method of a visualized electrochemiluminescence sensor based on a bipolar electrode array-microfluidic chip (preparation of a visualized lactic acid sensor based on a bipolar electrode array)

[0055] like figure 1 , figure 2 , image 3 As shown, a method for detecting lactic acid based on a bipolar electrode array-microfluidic chip visual electrochemiluminescence sensor, comprising steps:

[0056] 1) Preparation of coverslips

[0057] Mix the PDMS monomer and the curing agent at a mass ratio of 8:1-12:1 and stir evenly to obtain the PDMS mother liquor. Place the PDMS mother liquor at 4°C for 5-20 minutes, then degas for 20-40 minutes; pour it after degassing Balance on the silanized PDMS positive mold for 5-15 minutes, then cover with plastic wrap, heat and cure at 70-80°C for 1-2 hours; after curing, peel the PDMS from the silanized PDMS positive mold and cut into 3.0cm* A 1.2cm cover slip with a channel groove; use a 14G flat needle to proc...

Embodiment 2

[0069] Example 2 A visual electrochemiluminescence sensor based on a bipolar electrode array-microfluidic chip is used for the detection of lactic acid. A method for detecting lactic acid using a visual electrochemiluminescence sensor based on a bipolar electrode array-microfluidic chip is used to detect lactic acid on both sides of the cover slip. A platinum wire is respectively placed on the hole at the end as the driving electrode; the driving electrode provides a driving voltage of 8V through a DC stabilized power supply; 20 microliters of buffer solution containing the lactic acid solution to be tested and luminol are injected into the groove of the channel, wherein , the concentration of PBS is 0.01mol / L, the pH is 7.4, the concentration of lactic acid is 0.02mM-5mM, and the concentration of luminol is 1.3mmol / L; the lactate oxidase modified on the surface of the bipolar electrode catalyzes the reaction between dissolved oxygen and lactic acid to generate h 2 o 2 , and ...

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Abstract

The invention discloses a method for detecting lactic acid by a visual electrochemical luminescence sensor based on a bipolar electrode array-micro-fluidic chip, and belongs to the technical field of biosensors. Based on lactate oxidase / carbon nano tube / chitosan modified electrode, bipolar electrode and micro-fluidic chip technology, the lactic acid can be visually detected by electrochemical luminescence imaging. As a bipolar electrode is a conductive material capable of performing electrochemical reaction at the tail end without external electric contact, bulk array analysis is conveniently performed, mass production is facilitated, and detection cost is reduced. The electrochemical luminescence imaging technology has the advantages of high sensitivity, simple light path and the like and is used for visually detecting the lactic acid, the method is simple and convenient, and results are visual.

Description

technical field [0001] The invention relates to the technical field of biosensors, in particular to a method for detecting lactic acid with a visual electrochemiluminescence sensor based on a bipolar electrode array-microfluidic chip. Background technique [0002] Lactic acid is an organic base that is a precursor of acetolactate and a constituent of lecithin, also present in sphingomyelin. Lactic acid plays an important role in adjusting fat metabolism in animals, controlling cholesterol accumulation, preventing fatty liver, ensuring normal life activities of somatic cells, promoting cartilage development and normal operation of nervous system, etc. Its detection has important biological significance . [0003] Since lactic acid has no ultraviolet absorption and electrochemical activity, the catalyzed reaction using enzymes has become an important method for the determination of lactic acid. Most lactate sensors use peroxidase, with the help of electronic mediators or nan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00G01N21/76G01N27/26G01N27/30
CPCB01L3/502707B01L2200/10B01L2300/0645B01L2300/0663G01N21/76G01N27/26G01N27/30
Inventor 许林茹齐炼文肖依吕志敏
Owner CHINA PHARM UNIV
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