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Electrode and test paper for detecting creatinine by electrochemical method and preparation method of electrode and test paper

An electrochemical and creatinine technology, applied to test paper, can solve problems such as complex process, high sensor cost, creatine interference, etc., to achieve the effect of eliminating interference, improving anti-interference ability, and facilitating clinical testing or home monitoring

Pending Publication Date: 2021-09-07
PEKING UNIV FIRST HOSPITAL +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it has been found in practice that the blood filtration device set in the prior art not only makes the sensor cost high and the process complicated, but also cannot effectively avoid the interference of creatine in the blood on the detection of creatinine.

Method used

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  • Electrode and test paper for detecting creatinine by electrochemical method and preparation method of electrode and test paper
  • Electrode and test paper for detecting creatinine by electrochemical method and preparation method of electrode and test paper
  • Electrode and test paper for detecting creatinine by electrochemical method and preparation method of electrode and test paper

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] An enzyme electrode structure that can be used for electrochemical detection of creatinine, comprising an electrode material base layer; the outer surface of the electrode material base layer is covered with an electron mediator enhancement layer; the outer surface of the electron mediator enhancement layer is fixed with a An enzyme composition that reacts with creatinine to generate hydrogen peroxide. The base layer of the electrode material is composed of carbon paste doped with Prussian blue; the electron mediator enhancement layer is a Prussian blue layer modified on the base layer of the electrode material by electrochemical deposition; the enzyme composition is composed of creatinine Enzyme, creatinase and sarcosine oxidase are composed in a ratio of 5:2:1.25.

[0071]The method for preparing the enzyme electrode is as follows:

[0072] 1) Brush the carbon paste slurry doped with Prussian blue on the PET base plate to form strips with a thickness of 0.2 mm and dr...

Embodiment 2

[0079] A test paper capable of detecting creatinine by an electrochemical method, comprising a rectangular PET bottom plate, the surface of the PET bottom plate is provided with an electrode group and a circuit; the electrode group is composed of a long strip-shaped working electrode, a long strip shaped counter electrode and a strip-shaped reference electrode, the structure of the working electrode is the same as the electrode described in Example 1; the material of the counter electrode is made of carbon paste doped with Prussian blue; the described The reference electrode is a silver-silver chloride electrode.

Embodiment 3

[0081] Prepare a biosensor that can detect the creatinine level of the sample by electrochemical method, the process is as follows figure 1 shown, including:

[0082] (1) Select a rectangular PET material with a size of 25mm×30mm as the insulating base plate, and preset the respective positions of the working electrode, the counter electrode and the reference electrode on the insulating base plate;

[0083] (2) Make electrodes according to preset positions on the insulating base plate described in (1), that is, brush the carbon paste doped with Prussian blue to make the working electrode and the counter electrode, and brush with Ag / AgCl ink to obtain the reference electrode at the same time. electrode. The length, width and thickness of each electrode are 20mm, 2mm and 0.2mm respectively, such as figure 1 As shown in (2), the middle is the working electrode, the left is the counter electrode, and the right is the reference electrode; the insulating bottom plate coated with t...

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Abstract

The invention provides an electrode for detecting creatinine by an electrochemical method. The electrode comprises an electrode material base layer, the outer surface of the electrode material base layer is covered with an electron mediator enhancement layer; an enzyme composition capable of reacting with creatinine to generate hydrogen peroxide is fixed on the outer surface of the electron mediator enhancement layer; the electrode material base layer is composed of carbon paste or graphite containing Prussian blue; the enzyme composition is prepared from creatininase, creatinase and sarcosine oxidase according to the ratio of (3.75-6.25) to (1.25-2) to (0.75-1.25). The invention also provides test paper and a biosensor based on the electrode. The invention also provides a preparation method for preparing the electrode and the test paper, and a method for detecting the creatinine level by using the biosensor. The test paper provided by the invention can be used in various portable and rapid small electrochemical detection devices, and can realize rapid and convenient clinical detection or home monitoring of blood and urine creatinine.

Description

technical field [0001] The invention relates to a material for analyzing and testing clinical samples, in particular to an electrode for electrochemically detecting creatinine, a test paper, and a preparation method thereof. Background technique [0002] Currently, the number of people suffering from kidney disease, especially chronic kidney disease (CKD), is increasing all over the world. In 2017, the Global Burden of Disease, Trauma, and Risk Factors (GBD) study estimated the global prevalence of CKD to be 9.1%. Between 1990 and 2017, the CKD mortality rate across all age groups increased by 41.5% globally. Along with high morbidity and mortality, CKD imposes a huge burden on the global economy. Therefore, it is very necessary to monitor renal function regularly to prevent renal function damage from developing to an irreversible level. [0003] Creatinine is the most commonly used renal function monitoring indicator in clinical work. Creatinine (2-amino-1-methyl-5H-imi...

Claims

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Application Information

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IPC IPC(8): G01N27/327G01N27/30G01N27/26
CPCG01N27/3275G01N27/307G01N27/26
Inventor 崔悦杨莉吕继成
Owner PEKING UNIV FIRST HOSPITAL
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