Creatinine content determination method and creatinine diagnosis kit

A diagnostic kit and measurement method technology, applied in the field of medical testing and measurement, can solve problems such as slowness, inconvenient testing, and reduced accuracy of reagent testing, and achieve accurate and high-precision test results

Inactive Publication Date: 2006-05-10
王尔中
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

One of its disadvantages is that while the system generates the reduced nicotinamide coenzyme required for the reaction, it also offsets some of the enzymes such as alanine aminotransferase, aspartate aminotransferase, urea, ammonia, creatinine, and carbon dioxide. activity, causing the accuracy of the reagent test to be greatly reduced
The second disadvantage is that the reagent must be reacted for a period of time before the formal test in order to produce enough reduced nicotinamide coenzyme, which results in slow results and brings great inconvenience to the test

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0097] Embodiment one (single dose)

[0098] The creatinine diagnostic kit of the present embodiment comprises:

[0099] Buffer 80mmol / l

[0100]Coenzyme 1mmol / l

[0101] Creatinase 8000U / l

[0102] Creatinase 8000U / l

[0103] Sarcosine oxidase 6000U / l

[0104] Aldehyde dehydrogenase 8000U / l

[0105] Peroxidase 10000U / l

[0106] Stabilizer 50% (total volume)

[0107] 4-amino antiarsenic 2mmol / l

[0108] Carbolic acid 10mmol / l

[0109] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, test main wavelength 495-505nm, test sub-wavelength above 600nm, the volume ratio of the creatinine sample to the reagent to be tested is 1 / 25, and the reaction direction is positive reaction.

[0110] After the sample and reagents are added and allowed to mix, the following reactions occur:

[0111] Creatinine + water Creatinase creatine

[0112] Creatine + water creatinase Sarcosine + urea

[0113] Sarcosine + oxygen + water Sarcosine oxid...

Embodiment 2

[0118] Embodiment two (two doses)

[0119] The creatinine diagnostic reagent of the present embodiment has:

[0120] Reagent I

[0121] Buffer 100mmol / l

[0122] Coenzyme 3mmol / l

[0123] Creatinase 14000U / l

[0124] Sarcosine oxidase 8000U / l

[0125] Aldehyde dehydrogenase 14000U / l

[0126] Peroxidase 15000U / l

[0127] Stabilizer 50% (total volume)

[0128] 4-amino antiarsenic 1mmol / l

[0129] Reagent II

[0130] Buffer 100mmol / l

[0131] Creatinase 14000U / l

[0132] Stabilizer 50% (total volume)

[0133] 2,4,6-tribromo-3-hydroxy-benzenesulfonic acid 1mmol / l

[0134] When measuring creatinine content, the temperature is controlled at 30°C, the reaction time is 15 minutes, the main wavelength of the test is 546nm, the secondary wavelength of the test is above 630nm, the volume ratio of the creatinine sample to the reagent is 1 / 25, and the reaction direction is positive reaction.

[0135] The specific measurement steps are:

[0136] Creatinine + water Creatinase ...

Embodiment 3

[0143] Embodiment three (three doses)

[0144] The creatinine diagnostic reagent of the present embodiment is three doses, has:

[0145] Reagent I

[0146] Buffer 120mmol / l

[0147] Coenzyme 5mmol / l

[0148] Stabilizer 20mmol / l

[0149] 3-methyl-2-benzothiazolone hydrazone 2mmol / l

[0150] Reagent II

[0151] Buffer 120mmol / l

[0152] Creatinase 20000U / l

[0153] Sarcosine oxidase 10000U / l

[0154] Aldehyde dehydrogenase 20000U / l

[0155] Peroxidase 20000U / l

[0156] Stabilizer 50% (total volume)

[0157] Reagent III

[0158] Buffer 120mmol / l

[0159] Creatinase 20000U / l

[0160] Stabilizer 50% (total volume)

[0161] Dimethylaniline 2mmol / l

[0162] Set on the automatic biochemical analyzer: temperature 25°C, reaction time 20 minutes, test main wavelength 578nm, test sub-wavelength above 700nm, the volume ratio of the creatinine sample to be tested is 1 / 25, and the reaction direction is positive reaction.

[0163] The specific measurement steps are:

[0164] ...

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PUM

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Abstract

The invention relates to a method for determining the activity of creatinine, and also the reagent kit for creatinine diagnosis. The reagent kit comprises cushioning solution, coenzyme, creatinine enzyme, creatinase, creatine oxidase, peroxydase, aldehyde dehydrogenase, deacidized chromogen combination, and stabilizer. By mixing sample and reagent of a predetermiend volumetric ratio, generating coupling reaction between them, subjecting the final reactant to biochemiscal analyser, the main wavelength absorbancy variance ratio (speed) can be detected, and the creatinine content can thus be measured. The method of the invention can be used to obtain the needed measurement result purely through biochemical analytic instruments, and advantages of the method include higher sensibility, better accuracy, less susceptibility to contamination of internal or external materials, and easy application.

Description

technical field [0001] The invention relates to a method for measuring creatinine content, and at the same time, the invention also relates to a creatinine diagnostic kit for realizing the method, which belongs to the technical field of medical examination and measurement. Background technique [0002] Determination of creatinine mainly includes chemical determination method (Jaffe method), enzymatic method, high performance liquid chromatography and capillary electrophoresis. [0003] Chemical determination method - low cost, easy to operate, is currently one of the most commonly used methods for the determination of creatinine in China. The creatinine in the specimen interacts with picrate to form a yellow-red complex of picric acid and creatinine. The disadvantage of this method is that the specificity is not high, because vitamin C, acetone, acetoacetic acid, methyldopa and high concentration of glucose, protein and some antibiotics such as penicillin G, cefoxitin, cefa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/32
Inventor 王尔中
Owner 王尔中
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