48 results about "Aldehyde dehydrogenase (NAD+)" patented technology

A non-naturally occurring microbial organism has at least one exogenous nucleic acid encoding a MEK pathway enzyme expressed in a sufficient amount to produce MEK. The MEK pathway includes an enzyme selected from an acetoacetyl-CoA dehydrogenase (bifunctional), an acetoacetyl-CoA aldehyde dehydrogenase, a 3-oxobutyraldehyde reductase, a 3-oxobutanol dehydratase, an MEK oxidoreductase, a 3-oxobutyraldehyde aminotransferase, a 4-aminobutan-2-one deaminase, a 2-amino-4-ketopentanoate (AKP) thiolase, an AKP aminotransferase, a 2,4-dioxopentanoate decarboxylase, an AKP deaminase, an acetylacrylate decarboxylase, an AKP decarboxylase, a glutamate dehydrogenase, a 3-oxobutyraldehyde oxidoreductase (aminating) and an AKP oxidoreductase (aminating). A 2-butanol pathway further includes an MEK reductase. A method for producing MEK or 2-butanol includes culturing these organisms under conditions and for a sufficient period of time to produce MEK or 2-butanol.

The invention discloses a genetically engineered bacterium for expressing a human derived acetaldehyde dehydrogenase gene and application thereof, and belongs to the technical field of gene engineering and enzyme. The ALDH2 gene efficient expression component Trp1L-URA3-TPIp-ALDH2-TPIt-Trp1R is integrated into the genome of Saccharomyces cerevisiae W303-1A, the stability of gene expression is improved compared with free plasmid expression, a constitutive promoter is used, no induction agent is added during fermentation, and the product is relatively safe. When fermentation is performed for 96hours, the specific activity of producing acetaldehyde dehydrogenase reaches 0.97 U / mg. The specific activity of the producing acetaldehyde dehydrogenase reaches 6.256 U / mg in ethanol with the concentration of 250 mg / 100 mL, and the specific activity of the producing aldehyde dehydrogenase in a buffer with pH of 3 reaches 0.301 U / mg. The Saccharomyces cerevisiae is selected as a host strain, on the one hand, the activity of the enzyme is kept, on the other hand, product safety is improved, and a new approach and a theoretical basis are provided for the research and development of antialcoholicdrugs.

This invention relates to the bioproduction of substituted or unsubstituted phenylacetaldehyde, 2-phenylethanol, phenylacetic acid or phenylethylamine by subjecting a starting material comprising glucose, L-phenylalanine, substituted L-phenylalanine, styrene or substituted styrene to a plurality of enzyme catalyzed chemical transformations in a one-pot reaction system, using recombinant microbialcells overexpressing the enzymes. To produce phenylacetaldehyde from styrene, the cells are modified to overexpress styrene monooxygenase (SMO) and styrene oxide isomerase (SOI). To produce phenylacetic acid from styrene, SMO, SOI and aldehyde dehydrogenase are overexpressed. Alternatively, to produce 2-phenylethanol, SMO, SOI and aldehyde reductase or alcohol dehydrogenase are overexpressed, while to produce phenylethylamine, SMO, SOI and transaminase are overexpressed.

The invention discloses a recombinant strain for producing a 3-hydracrylic acid homopolymer and / or a 3-hydracrylic acid copolymer and an application thereof. The construction method of the recombinant strain comprises the following steps: leading 1,3-Propanediol dehydrogenase coded genes, aldehyde dehydrogenase coded genes, 3-hydracrylic acid coenzyme A ligase coded genes and PHA (Polyhydroxyalkanoates) polymerase coded genes into a starting strain to obtain the recombinant strain. The experiments in the invention prove that the engineering bacteria can efficiently express 3-hydracrylic acid coenzyme A ligase coded genes and PHA polymerase coded genes and enable the 3-hydracrylic acid to be finally polymerized into 3-hydracrylic acid homopolymer (P(3HP)) from the 3-hydracrylic acid coenzyme A. Minitype fermentation tank experiments show that the engineering bacteria provided by the invention can have a maximum P (3HP) output of 8.9g / L after being fermented in a 6L fermentation tank and the P (3HP) can account for a maximum 91.5% of cell dry weight. In addition, the recombinant strain provided by the invention has the advantages of simple production process, low costs and broad application prospects.

A microorganism capable of producing acrylic acid, comprising a genetic modification that increases activity of CoA acylating aldehyde dehydrogenase (ALDH) catalyzing conversion of 3-hydroxypropionaldehyde (3-HPA) to 3-hydroxy propionyl-CoA (3-HP-CoA) and a genetic modification that increases activity of 3-HP-CoA dehydratase catalyzing conversion of 3-HP-CoA to acrylyl-CoA in the microorganism in comparison with a cell that is not genetically engineered; as well as a method of producing the microorganism, and a method of producing acrylic acid using the same.

The invention relates to a method for determining the activity of creatinine, and also the reagent kit for creatinine diagnosis. The reagent kit comprises cushioning solution, coenzyme, creatinine enzyme, creatinase, creatine oxidase, peroxydase, aldehyde dehydrogenase, deacidized chromogen combination, and stabilizer. By mixing sample and reagent of a predetermiend volumetric ratio, generating coupling reaction between them, subjecting the final reactant to biochemiscal analyser, the main wavelength absorbancy variance ratio (speed) can be detected, and the creatinine content can thus be measured. The method of the invention can be used to obtain the needed measurement result purely through biochemical analytic instruments, and advantages of the method include higher sensibility, better accuracy, less susceptibility to contamination of internal or external materials, and easy application.

The invention provides a construction method of a synthetic strain for generating fengycin by transforming xylose through regulating and controlling a Dahms pathway. The construction method comprises the following steps of: connecting a promoter gene P43, xylose dehydrogenase xylB, xylC, a xylosic acid lactonase gene yjhG and a 2-ketone-3-deoxyxylosic acid dehydratase gene yjhH in series to prepare a Dahms xylose pathway module into a shuttle vector pHY300PLK; meanwhile, starting from metabolite glycolaldehyde of the Dahms pathway, connecting an aldehyde dehydrogenase gene aldA, malic acid synthetase aceB, malic acid dehydrogenase mdh and the promoter P43 in series to prepare an glycolaldehyde back-complement TCA regulating and controlling module into a shuttle vector pHP13; co-expressing the two shuttle vectors in a chassis strain to construct a fengycin high-quality strain BSU02 which is synthesized by co-expressing and transforming the xylose through the Dahms module and the glycolaldehyde back-complement TCA regulating and controlling module.

The invention belongs to the field of medical detection, and in particular relates to a new application of acetaldehyde dehydrogenase 2 gene. The invention discloses the use of the acetaldehyde dehydrogenase 2 gene as a detection index for gastric cancer molecular typing, and also discloses the use of anthracyclines in the preparation of acetaldehyde dehydrogenase 2 gene mutation or deletion type gastric cancer treatment drugs. For the first time, it was found that the deletion or mutation of the ALDH2 gene caused gastric cancer cells to show different sensitivities to tumor chemotherapy drugs, and the in vivo experiments were verified, providing a new detection index for the molecular typing of gastric cancer. The application of the aldehyde dehydrogenase 2 gene mutation or deletion type gastric cancer treatment drug provides a new option for individualized treatment of gastric cancer.

This disclosure concerns nucleic acid molecules and methods of use thereof for control of pathogens through RNA interference-mediated inhibition of target coding and transcribed non-coding sequences in pathogens. The disclosure also concerns methods for applying dsRNA through formulations and / or transgenic plants that express nucleic acid molecules useful for the control of pathogens, and the plant cells and plants obtained thereby.

The invention discloses recombinant bacteria using xylose to produce glycollic acid and a building method and application of the recombinant bacteria and belongs to the technical field of genetic engineering. The xylose dehydrogenase gene, xylonic acid lactonase gene, xylonic acid dehydratase gene, 3-deoxygenation-D-glycerin ketopentose acid aldolase gene and glycolic aldehyde dehydrogenase gene in the recombinant bacteria using the xylose to produce the glycollic acid are overexpressed. Meanwhile, the invention further provides a preparation method of the recombinant bacteria and a method using the recombinant bacteria to produce the glycollic acid. By the recombinant bacteria, the biological synthesizing path using D-xylose as the carbon source to form the glycollic acid through glycolic aldehyde conversion is achieved for the first time.

Methods are provided for obtaining a sample of β cells from an isolated donor pancreas or isolated pancreatic islets and analyzing the sample using flow cytometry to determine the percentage of β cells in the sample that express detectable levels of ALDH1 A3. If the percentage of ALDH1 A3-expressing β cells in the sample is about 3% or lower, then it is possible to determine that the pancreas or islets are healthy enough for implantation into a subject, and implanting the pancreas or islets. If the percentage of ALDH1 A3-expressing cells is above about 5%, then it is determined that the pancreas or islets are not suitable for implantation into the subject and discarding the pancreas or islets. Isolated non-insulin-producing or low-insulin-producing pancreatic beta cells are also provided.

The invention provides recombinant bacteria capable of synthesizing 3-hydracrylic acid by using glucan as well as a construction method and application of the recombinant bacteria, and belongs to the field of gene engineering and fermentation engineering. Klebsiella pneumoniae is host bacteria for expressing a gene lgk capable of encoding levodextran kinase and a gene aldH capable of encoding aldehyde dehydrogenase. The construction method comprises the following steps: obtaining a recombinant carrier puC18-lgk-aldH, converting the recombinant carrier puC18-lgk-aldH into a competent cell of the host Klebsiella pneumoniae, and obtaining the recombinant bacteria. By use of the levodextran kinase gene lgk and the aldehyde dehydrogenase gene aldH which are optimized through a codon, in combination with the whole scheme, the substrate use rate is increased, and the yield is increased.

The invention relates to the fields of genetic engineering technology and biocatalysis, and discloses aldehyde dehydrogenase and its gene, construction of recombinant bacteria and its application in furan carboxylic acid synthesis. The present invention screens out five aldehyde dehydrogenases from Comamonas testosteroni SC1588 through gene mining technology, and its amino acid sequences are SEQ ID.1, SEQ ID.2, SEQ ID.3, and SEQ ID .4 or shown in SEQ ID.5. The five aldehyde dehydrogenases of the present invention can efficiently catalyze the selective oxidation of bio-based furan to synthesize the corresponding furan carboxylic acid, the yield of the target product is as high as about 90%, and the highest yield can even reach 100%; the space-time yield reaches 1.9g / L h. The invention has simple production process, mild production conditions and high production efficiency.

The invention belongs to the technical field of medicinal chemistry, and particularly relates to an amide aldehyde dehydrogenase agonist as well as a synthesis method and application thereof. The amide compounds provided by the invention show relatively high agonistic activity to ALDH2, so that the compounds have the potential of preparing medicines for treating diseases related to ALDH2 activity; compared with a positive control, the amide compound has the advantages that the activity and the water solubility are obviously improved, and the druggability is better. Therefore, the compound provided by the invention has a good development prospect.

The invention discloses a recombinant Escherichia coli which utilizes L-lactic acid to synthesize S-1,2-propanediol and a construction method thereof. The method comprises the following steps: 1) replacing the lldD gene, the adheE gene and the ackA-pta gene in Escherichia coli BW25113-△poxB with the encoding gene of 3-hydroxypropionate dehydrogenase and the coenzyme A-dependent succinate half The coding gene of aldehyde dehydrogenase and the coding gene of propionyl-CoA transferase, the obtained recombinant strain is marked as BWPDO1; 2) knock out the ldhA gene and dld gene of said BWPDO1, and obtain the recombinant strain BWPDO2; 3) introduce the The coding gene of pyruvate decarboxylase and the coding gene of NAD-dependent acetaldehyde coenzyme A dehydrogenase were introduced into BWPDO2 to obtain recombinant bacteria BWPDO3, which was the recombinant Escherichia coli. Experiments have proved that the recombinant Escherichia coli obtained in the present invention can use L-lactic acid to transform into S-1,2-propanediol, and use 200mM sodium lactate as a substrate to produce 3.4mM S-1,2-propanediol when cultured at 37°C for 24 hours. The invention lays the foundation for the work of improving the yield and conversion rate of biosynthetic S-1,2-propanediol.

The invention discloses a combined expression vector for expressing trans-chrysanthemic acid and a construction method and application of the combined expression vector, the combined expression vector is an expression cassette with a plant expression vector as a skeleton and containing an MCPI promoter driving TcCDS gene and an expression cassette containing an MCPI promoter driving fusion gene ADH2 / ALDH1, the TcCDS gene is a gene for coding inulin diphosphate synthase, and the fusion gene ADH2 / ALDH1 is a gene for coding trans-chrysanthemic acid. The fusion gene ADH2 / ALDH1 is a fusion gene formed by a gene TcADH2 for coding alcohol dehydrogenase 2 and a gene TcALDH1 for coding aldehyde dehydrogenase 1 through a DNA (Deoxyribose Nucleic Acid) linker, and the MCPI promoter is a glandular hair specific metal carboxypeptidase inhibitor promoter. According to the invention, a trans-chrysanthemic acid biosynthesis pathway is reconstructed in the tomato type VI glandular hair, a large amount of trans-chrysanthemic acid is generated, and the result shows that the tomato type VI glandular hair is an efficient platform for biosynthesis of trans-chrysanthemic acid through metabolic engineering.