Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Creatinine content determination method and creatinine diagnosis kit

A diagnostic kit and measurement method technology, which is applied in the field of medical testing and measurement, can solve the problems of slowness, inconvenient testing, and reduced accuracy of reagent testing, and achieve the effect of good accuracy and accurate test results

Inactive Publication Date: 2006-05-10
王尔中
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

One of its disadvantages is that while the system generates the reduced nicotinamide coenzyme required for the reaction, it also offsets some of the enzymes such as alanine aminotransferase, aspartate aminotransferase, urea, ammonia, creatinine, and carbon dioxide. activity, causing the accuracy of the reagent test to be greatly reduced
The second disadvantage is that the reagent must be reacted for a period of time before the formal test in order to produce enough reduced nicotinamide coenzyme, which results in slow results and brings great inconvenience to the test

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0108] Embodiment one (single agent)

[0109] The creatinine diagnostic kit of the present embodiment comprises:

[0110] Buffer 80mmol / l

[0111] Pyruvate 3mmol / l

[0112] Phosphoenolpyruvate 2mmol / l

[0113] Creatininase 5000U / l

[0114] Creatinase 5000U / l

[0115] Urease 5000U / l

[0116] Phosphoenolpyruvate Carboxylase 1000U / l

[0117] Pyruvate oxidase 5000U / l

[0118] Peroxidase 8000U / l

[0119] Stabilizer 50% (total volume)

[0120] 4-amino antiarsenic 2mmol / l

[0121] Carbolic acid 10mmol / l

[0122] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, test main wavelength 495-505nm, test sub-wavelength above 600nm, the volume ratio of the creatinine sample to the reagent to be tested is 1 / 25, and the reaction direction is positive reaction.

[0123] After the sample and reagents are added and allowed to mix, the following reactions occur:

[0124] Creatinine + water Creatinase Creatine

[0125] Creatine + water creatina...

Embodiment 2

[0133] Embodiment two (two doses)

[0134] The creatinine diagnostic reagent of the present embodiment has:

[0135] Reagent I

[0136] Buffer 100mmol / l

[0137] Pyruvate 5mmol / l

[0138] Phosphoenolpyruvate 6mmol / l

[0139] Creatinase 7500U / l

[0140] Urease 7500U / l

[0141] Phosphoenolpyruvate Carboxylase 4000U / l

[0142] Pyruvate oxidase 7500U / l

[0143] Peroxidase 14000U / l

[0144] Stabilizer 50% (total volume)

[0145] 4-amino antiarsenic 1mmol / l

[0146] Reagent II

[0147] Buffer 100mmol / l

[0148] Creatinase 7500U / l

[0149] Stabilizer 50% (total volume)

[0150] 2,4,6-tribromo-3-hydroxy-benzenesulfonic acid 1mmol / l

[0151] When measuring creatinine content, the temperature is controlled at 30°C, the reaction time is 15 minutes, the main wavelength of the test is 546nm, and the secondary wavelength of the test is 630nm. The volume ratio of the creatinine sample to the reagent is 1 / 25, and the reaction direction is positive reaction.

[0152] The specifi...

Embodiment 3

[0162] Embodiment three (three doses)

[0163] The creatinine diagnostic reagent of the present embodiment is three doses, has:

[0164] Reagent I

[0165] Buffer 120mmol / l

[0166] Pyruvate 7mmol / l

[0167] Phosphoenolpyruvate 10mmol / l

[0168] Stabilizer 20mmol / l

[0169] 3-methyl-2-benzothiazolone hydrazone 2mmol / l

[0170] Reagent II

[0171] Buffer 120mmol / l

[0172] Creatinase 10000U / l

[0173] Urease 10000U / l

[0174] Phosphoenolpyruvate Carboxylase 7000U / l

[0175] Pyruvate oxidase 10000U / l

[0176] Peroxidase 20000U / l

[0177] Stabilizer 50% (total volume)

[0178] Reagent III

[0179] Buffer 120mmol / l

[0180] Creatinase 10000U / l

[0181] Stabilizer 50% (total volume)

[0182] Dimethylaniline 2mmol / l

[0183] Set on the automatic biochemical analyzer: temperature 25°C, reaction time 20 minutes, test main wavelength 578nm, test sub-wavelength above 700nm, the volume ratio of the creatinine sample to the reagent to be tested is 1 / 25, and the reaction dir...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for determining the activity of creatinine, and also the reagent kit for creatinine diagnosis. The reagent kit comprises cushioning solution, pyroracemic acid, phosphoenolpyruvate phosphatase, creatinine enzyme, creatinase, urease, phosphoenolpyruvate carboxylase, pyruvic oxidase, peroxydase, stabilizer, and deacidized chromogen combination. By mixing sample and reagent of a predetermiend volumetric ratio, generating coupling reaction between them, subjecting the final reactant to biochemiscal analyser, the main wavelength absorbancy variance ratio (speed) can be detected, and the creatinine content can thus be measured. The method of the invention can be used to obtain the needed measurement result purely through biochemical analytic instruments, and advantages of the method include higher sensibility, better accuracy, less susceptibility to contamination of internal or external materials, and easy application.

Description

technical field [0001] The invention relates to a method for measuring creatinine content, and at the same time, the invention also relates to a creatinine diagnostic kit for realizing the method, which belongs to the technical field of medical examination and measurement. Background technique [0002] Determination of creatinine mainly includes chemical determination method (Jaffe method), enzymatic method, high performance liquid chromatography and capillary electrophoresis. [0003] Chemical determination method - low cost, easy to operate, is currently one of the most commonly used methods for the determination of creatinine in China. The creatinine in the specimen interacts with picrate to form a yellow-red complex of picric acid and creatinine. The disadvantage of this method is that the specificity is not high, because vitamin C, acetone, acetoacetic acid, methyldopa and high concentration of glucose, protein and some antibiotics such as penicillin G, cefoxitin, cefa...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/28
Inventor 王尔中
Owner 王尔中
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com