Creatinine detection reagent capable of resisting interference of medicines such as calcium dobesilate

An anti-calcium dobesilate and detection reagent technology, which is applied in the direction of material analysis by observing the impact on chemical indicators, and analysis by making materials undergo chemical reactions, can solve the negative deviation and interference of creatinine determination kits; China's rural medicine, creatinine detection interference and other issues, to achieve the effect of strong anti-drug interference, high accuracy, and guaranteed accuracy

Active Publication Date: 2020-10-13
WUHAN HANHAI NEW ENZYMES BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, there are also many literature reports that calcium 2,5-hydroxybenzenesulfonate has serious negative interference on the detection of creatinine by enzymatic method, for example, clinical research on the influence of calcium dobesilate on the detection of serum creatinine by oxidase method; contemporary medicine; Song Jingfang, Pan Benyou, Wu Xianding et al.; 2019,25(09):175-177 studied the interference of calcium dobesilate to the enzymatic detection of creatinine, and found that calcium dobesilate would cause the creatinine assay kit (sarcosine oxidase Negative deviation; Calcium dobesilate interferes with the detection of creatinine by sarcosine oxidase method and picric acid method; Chinese Rural Medicine; Dai Yingjie, Zhou Yong, Chen Wenju, Li Zhaoyun; DOI: 10.19542 / j.cnki.1006- 5180.000833 studied the effect of calcium dobesilate on the detection of creatinine by sarcosine oxidase method and picric acid method, and found that calcium dobesilate has a large negative interference on the detection of creatinine by sarcosine oxidase method
In addition, the researchers found that there are many drugs that interfere with creatinine detection, for example, phensulfame

Method used

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  • Creatinine detection reagent capable of resisting interference of medicines such as calcium dobesilate

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The reagents provided in this embodiment include a first reagent and a second reagent, wherein the first reagent includes 10 g / L of 3-(N-morpholino)propanesulfonic acid, 0.6 g / L of TOOS, and 8 mmol / L of piperidine oxygen free radicals. L, gentamicin 0.1g / L, sarcosine oxidase 15KU / L, creatinase 40KU / L, ascorbate oxidase 5KU / L and catalase 300KU / L, and the pH of the first reagent is 7.6 ;

[0022] The second reagent includes 3-(N-morpholino)propanesulfonic acid 10g / L, 4-AAP 0.2g / L, creatinase 400KU / L, peroxidase 5KU / L, preservative 0.5g / L and sub Potassium ferricyanide is 0.3 g / L, and the pH of the second reagent is 7.6.

[0023] The first reagent and the second reagent adopt liquid two-reagent colorimetric method to test the content of creatinine.

Embodiment 2

[0025] The reagent provided in this embodiment includes a first reagent and a second reagent, wherein the first reagent includes 10 g / L of 3-(N-morpholino)propanesulfonic acid, 0.6 g / L of TOOS, and 5 mmol / L of piperidine oxygen free radical. L, gentamicin 0.1g / L, sarcosine oxidase 15KU / L, creatinase 40KU / L, ascorbate oxidase 5KU / L and catalase 300KU / L, and the pH of the first reagent is 7.6 ;

[0026] The second reagent includes 3-(N-morpholino)propanesulfonic acid 10g / L, 4-AAP 0.2g / L, creatinase 400KU / L, peroxidase 5KU / L, preservative 0.5g / L and sub Potassium ferricyanide is 0.2 g / L, and the pH of the second reagent is 7.6.

[0027] The first reagent and the second reagent adopt liquid two-reagent colorimetric method to test the content of creatinine.

Embodiment 3

[0029] The reagent provided in this embodiment includes a first reagent and a second reagent, wherein the first reagent includes 3-(N-morpholino)propanesulfonic acid 10g / L, TOOS 0.6g / L, gentamicin 0.1g / L L, sarcosine oxidase 15KU / L, creatinase 40KU / L, ascorbate oxidase 5KU / L and catalase 300KU / L, and the pH of the first reagent is 7.6;

[0030] The second reagent includes 3-(N-morpholino)propanesulfonic acid 10g / L, 4-AAP 0.2g / L, piperidinyl oxygen free radical 2mmol / L, creatinase 400KU / L, peroxidase 5KU / L , preservative 0.5g / L, potassium ferrocyanide 0.2g / L, and the pH of the second reagent is 7.6.

[0031] The first reagent and the second reagent adopt liquid two-reagent colorimetric method to test the content of creatinine.

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Abstract

The invention discloses a creatinine detection reagent capable of resisting interference of medicines such as calcium dobesilate. The creatinine detection reagent comprises a first reagent and a second reagent, wherein the first reagent comprises 3-(N-morpholinyl)propanesulfonic acid, TOOS, gentamicin, sarcosine oxidase, creatinase, ascorbic acid oxidase and catalase, and the second reagent comprises 3-(N-morpholinyl)propanesulfonic acid, 4-AAP, creatininase amidohydrolase, peroxidase, preservative and potassium ferrocyanide; the first reagent and/or the second reagent further comprise/comprises piperidine oxygen radicals. According to the creatinine detection reagent provided by the invention, the interference effect of medicines such as calcium dobesilate can be reduced, the accuracy ofcreatinine detection in clinical detection is ensured, and a creatinine detection method with a wide linear range, strong medicine interference resistance and high accuracy is realized.

Description

technical field [0001] The invention relates to the technical field of detection reagents, in particular to a creatinine detection reagent resistant to drug interference such as calcium dobesilate. Background technique [0002] The creatinine level in the blood reflects the filtration function of the glomerulus and is one of the important indicators to measure the renal function. However, clinically, it is often found that the test results of serum creatinine do not match the clinical manifestations of patients. With the continuous development of science and technology, researchers have found that the error in the test results of serum creatinine is due to the interference of drugs. [0003] Among them, calcium 2,5-hydroxybenzenesulfonate, as a vascular protective drug, can reduce vascular resistance by adjusting the physiological function of the microvascular wall, and is mainly used in patients with diabetes, nephropathy, chronic renal insufficiency, etc., and the usage is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 蔡锦刚崔鹏飞王晨熊佩
Owner WUHAN HANHAI NEW ENZYMES BIOLOGICAL TECH CO LTD
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