Method for improving heat stability of creatinase

A thermal stability, creatinase technology, applied in the fields of genetic engineering and enzyme engineering, can solve the problems of poor stability, large amount of creatinase, no chemical bond formation, etc., and achieves convenient application, improved thermal stability, and thermal stability. Sex-enhancing effect
CN106399283AActive Publication Date: 2017-02-15JIANGNAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
JIANGNAN UNIV
Publication Date
2017-02-15

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Abstract

The invention discloses a method for improving the heat stability of creatinase, and belongs to the fields of genetic engineering and enzyme engineering. According to the method, a mutant is obtained by carrying out amino-acid substitution and mutation on the basis of the creatinase from a Pseudomonas putida source. By comparing the mutant H125C / L130C provided by the invention with wild-type creatinase, the Tm value is increased by 8.2 DEG C, and the heat stability is obviously improved. Through the method provided by the invention, the heat stability of the creatinase is effectively improved.
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Description

technical field

[0001] The invention relates to a method for improving the thermal stability of creatinase, belonging to the fields of genetic engineering and enzyme engineering. Background technique

[0002] Creatinase (creatinase; E 3.5.3.3; CRE; classification name creatine aminohydrolase, creatinaseamidinohydrolase) belongs to hydrolase, catalyzes the hydrolysis of creatine to produce urea and sarcosine. Creatine hydrolase is the key enzyme in the creatine content determination method by sarcosine oxidase method.

[0003] As one of the indicators for clinical renal function monitoring, creatinine has always attracted much attention. With the development of science, its determination method has evolved from the original alkaline picric acid method (Jeffe's reaction) to the enzymatic method, which can be said to be a huge progress, because the latter can overcome some inherent shortcomings of the former and is widely used . The three tool enzymes involved in creatine de...

Claims

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