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Detection kit for measuring creatinine through enzyme method and using method thereof

A technology for detection kits and kits, applied in measuring devices, color/spectral characteristic measurement, and analysis through chemical reactions of materials, etc., can solve problems such as interference of colorimetric detection methods, interference of anti-oxidative reaction systems, etc. , to achieve good solubilization effect, avoid metal ion interference, and high stability

Active Publication Date: 2019-07-23
DALIAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Bilirubin not only interferes with the color contrast detection method due to its color characteristics, but also interferes with the reaction system indicated by the Tinder reaction because of its antioxidant properties

Method used

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  • Detection kit for measuring creatinine through enzyme method and using method thereof
  • Detection kit for measuring creatinine through enzyme method and using method thereof
  • Detection kit for measuring creatinine through enzyme method and using method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Preparation of sarcosine oxidase

[0053] Step 1. Obtain the target sequence

[0054]Using Bacillus CGMCC No.17306 as the starting strain, the whole genome was detected and compared with the existing sarcosine oxidase gene to obtain the original sarcosine oxidase gene, such as Figure 4 As shown, the gene sequence coding for sarcosine oxidase derived from microorganisms is modified and fully synthesized, as shown in SEQ No.1, and the amino acid sequence encoded by sarcosine oxidase is shown in SEQ No.2.

[0055] The preservation information of the starting strain is: a marine bacterium LYH18 strain, Bacillus sp., which has been preserved in the General Microbiology Center of the China Committee for the Collection of Microbial Cultures on March 6, 2019, address: Beijing Institute of Microbiology, Chinese Academy of Sciences, No. 1, Courtyard 1, Beichen West Road, Chaoyang District, City, Zip Code: 100101, and its biological deposit number is CGMCC No.17306.

[0056] St...

Embodiment 2

[0077] A detection kit for the enzymatic determination of creatinine in this embodiment, the specific raw materials and proportioning are as follows:

[0078] The reagent group H1 comprises:

[0079]

[0080] The reagent group H2 comprises:

[0081]

Embodiment 3

[0083] A detection kit for the enzymatic determination of creatinine in this embodiment, the specific raw materials and proportioning are as follows:

[0084] The reagent group H1 comprises:

[0085]

[0086] The reagent group H2 comprises:

[0087]

[0088]

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Abstract

The invention relates to the field of biochemical detection, in particular to a detection kit for measuring creatinine through an enzyme method and a using method thereof. The kit comprises various reagents of sarcosine oxidase, creatinase, catalase, a buffer solution and the like prepared by using a genetically engineered bacterium GLYH18 prepared by a LYH18 bacterial strain with the preservationnumber of CGMCC No.17306, the sample is mixed with the reagents, and then, the final reactant is placed under a biochemical analyzer to detect the change condition of absorbance so as to measure thecontent of creatinine. The preparation method of the sarcosine oxidase in the kit is simple and low in cost, the content of creatinine in the sample can be quickly and accurately measured by the kit with the enzyme added, the kit can be kept stable at room temperature, which facilitates practical application and better facilitates industrial production.

Description

technical field [0001] The invention relates to the field of biochemical detection, in particular to a detection kit for enzymatic determination of creatinine and a use method thereof. Background technique [0002] The etiology of kidney disease is complex. Since the pathogenesis of most "primary" glomerular diseases is not fully understood, the current treatment of glomerular diseases is mostly empirical treatment, and there is a lack of etiological treatment. In the field of glomerular disease treatment, prospective, randomized controlled clinical treatment trials are very limited. Nevertheless, looking back over the past three decades, some landmark treatments have been used clinically at each stage, and have improved the treatment level of kidney diseases to a certain extent. [0003] The main clinical manifestations of kidney disease are proteinuria, hematuria, edema, hypertension, renal insufficiency, etc. Over the years, clinicians have conducted comprehensive analy...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N21/31
CPCG01N21/31G01N21/78G01N2021/775
Inventor 迟乃玉刘洋张庆芳于爽希伦
Owner DALIAN UNIV
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