Water soluble compositions incorporating enzymes, and method of making same

An enzyme composition, water-soluble technology, applied in the direction of detergent composition, organic cleaning composition, chemical instruments and methods, etc., can solve the problems of low enzyme activity and reduced storage time

Active Publication Date: 2014-11-26
MONOSOL LLC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Enzyme activity in previously known membranes tends to be very low and decreases with storage time

Method used

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  • Water soluble compositions incorporating enzymes, and method of making same
  • Water soluble compositions incorporating enzymes, and method of making same
  • Water soluble compositions incorporating enzymes, and method of making same

Examples

Experimental program
Comparison scheme
Effect test

example

[0136] Colorimetric measurement of protease activity

[0137] The protease used is known to catalyze N,N-dimethylcasein (DMC). By hydrolysis of peptide bonds, carboxylic acids and primary amines are produced. The resulting amine reacts with trinitrobenzenesulfonic acid (TNBS) under basic conditions to form a colored complex. Colored complexes are detected at 405nm. The reaction between primary amines and TNB is assumed to be faster than the cleavage of DMC. Therefore, the reaction in the presence of excess substrate can be assumed to be a function of enzyme concentration only.

[0138] Protease activity was determined relative to enzyme standards. A standard is a sample of the raw material protease composition used to prepare the membrane composition according to the examples herein. Results are reported in convenient units of enzyme activity such as catal (Eur. J. Biochemistry, Vol. 97, 319-320, 1979). Using enzymes of known activity, prepare a set of standards by seri...

example 1

[0146] Using the ingredients identified in the table below and in the instructions, a set of water soluble films was prepared in the amounts indicated. Amounts are expressed in phr unless otherwise stated.

[0147]

[0148] Each membrane also includes about 35phr plasticizer, 390phr water and small amounts of one or more processing aids which are not considered to interfere with enzyme recovery, such as antiblocking agents, surfactants, caustic soda, membrane Forming agents, anti-foaming agents, anti-yellowing agents, etc., in amounts totaling less than about 2.5 phr. A water soluble film according to Formulation 1 was prepared as follows. In a beaker, the processing aid and water were agitated for 10 minutes. A water-soluble resin is added to the mixture, and the mixture is heated. The solution was boiled for 30 minutes. The solution was degassed overnight in an oven at 65 °C. The enzyme, plasticizer and additional water are mixed into the solution at a temperature no...

example 2

[0152] Using the ingredients identified in the table below and in the instructions, a set of water soluble films was prepared in the amounts indicated. Amounts are expressed in phr unless otherwise stated.

[0153]

[0154] Each membrane also includes approximately 35phr plasticizer, 400phr water and small amounts of one or more processing aids which are not considered to interfere with enzyme recovery, such as antiblocking agents, surfactants, caustic soda, membrane Forming agents, defoamers, anti-yellowing agents, etc., in amounts totaling less than about 6 phr. Follow the operation of Example 1. The percent recovery of enzyme activity in water-soluble films based on vinyl alcohol / methyl acrylate copolymers was determined as follows. The water soluble membrane without enzyme substrate, Membrane 3, demonstrated 41% recovery of protease activity. The water soluble membrane containing the protease substrate, membrane 4, demonstrated 84% recovery of protease activity. In ...

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PUM

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Abstract

Disclosed herein are water soluble compositions, such as films, including a mixture of a first water-soluble resin, an enzyme, and an enzyme stabilizer which comprises a functional substrate for the enzyme, methods of making such compositions, and methods of using such compositions, e.g. to make packets containing functional ingredients. The enzymes can include proteases and mixtures of proteases with other enzymes, and the compositions provide good retention of enzyme function following film processing and storage.

Description

technical field [0001] The present disclosure generally relates to water soluble films. More specifically, the present disclosure relates to water soluble films comprising a mixture of a first water soluble resin, an enzyme, and an enzyme stabilizer comprising a functional substrate for the enzyme. Background technique [0002] Water soluble films are well known in the art. Water soluble films have many applications, such as packaging materials, where the film itself can constitute a water soluble pack. These compositions have the advantage of being easy to dose, handle, transport and store. [0003] Water soluble packets are ideal for single dose applications where the packet is encapsulated into laundry detergent, bleach or other laundry additives, fabric care, dishwashing, hard surface cleaning, beauty care, skin care, other personal care or food products combination. Often enclosed in water soluble packets are active agents that can become unstable in the presence of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11D17/04
CPCC11D3/222C11D17/042C11D3/38627C11D3/2093C11D3/3761C11D3/378C11D3/38645C11D3/38C11D3/38636C11D3/225C11D3/38609C11D3/386B29D7/01C08J5/18C08K5/0016C08L29/04C08L31/04C08L33/04C08L101/14
Inventor 大卫·M·李詹妮弗·L·希姆希
Owner MONOSOL LLC
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